Data/hora: 11/06/2024 13:46:41

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 22/05/2007
Data da última atualização: | 03/09/2015
Autoria: | SILVA, C. M. M. de S.; MELO, I. S. de; OLIVEIRA, P. R.
Afiliação: | Celia Maria Maganhotto de Souza Silva, Embrapa Meio Ambiente; ITAMAR SOARES DE MELO, CNPMA; Pablo Roberto de Oliveira.
Título: | Ligninolytic enzyme production by Ganoderma spp.
Ano de publicação: | 2005
Fonte/Imprenta: | Enzyme and Microbial Technology, Amsterdam, v. 37, p. 324-329, 2005.
Idioma: | Inglês
Conteúdo: | The objective of this work was to quantify the activities of lignin peroxidase, manganese peroxidase and laccase produced by the white-rot basidiomicete Ganoderma spp. Initially, four strains were cultured in the presence of the Remazol Brilliant Blue (RBBR) to evaluation with regard to their ligninolytic potential by decolorization of the dye. Two strains that showed the best ligninolytic potential GASI3.4, and CCB209 were cultured in liquid culture medium, added or not of wheat bran (4.5g) and/or propanil (2.5 ug mL-1) to determine the enzymatic activities. The cultures were incubated for 7 days at 30ºC, and after they were filtered and utilized for determination of the enzymatic activities of lignin peroxidase, manganese peroxidase and laccases. Two Ganoderma sp. strains showed differences in enzymatic activity depending on culture condition, in spite of having similar protein secretion patterns and biomass yields. In general, the presence of wheat bran induced a high production of the enzymes, while the presence of herbicide inhibited the activities of LiP and MnP only in the strain GASI3.4.
Palavras-Chave: | Basidiomiceto; Enzimas lignolíticas; Farelo de trigo; Linhagens.
Thesagro: | Atividade enzimática; Produção; Propanil.
Categoria do assunto: | W Química e Física
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (6862-1) - UPC - AP - DD -  -  - 2005AP_019 - DIS