Data/hora: 08/06/2024 06:45:26

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 27/06/2013
Data da última atualização: | 19/05/2023
Autoria: | SILVA, A. dos S.; OLIVEIRA, E. J. de; HADDAD, F.; JESUS, O. N. de; OLIVEIRA, S. A. S. de; COSTA, M. A. P. de C.
Afiliação: | ALINE DOS SANTOS SILVA, UFRB; EDER JORGE DE OLIVEIRA, CNPMF; FERNANDO HADDAD, CNPMF; ONILDO NUNES DE JESUS, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA, UFRB.
Título: | Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers.
Ano de publicação: | 2013
Fonte/Imprenta: | Scientia Agricola, v.70, n.2, p.108-115, March/April 2013.
ISSN: | 0103-9016
Idioma: | Português
Conteúdo: | Fusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder & H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of discrimination of the AFLP primer combinations, with the presence/absence of up to 26 specific fragments per isolate. Thus, specific fingerprinting was obtained for 10 of the 15 isolates analyzed.
Palavras-Chave: | Passion fruit.
Thesagro: | Fusarium; Maracujá; Marcador Molecular.
Thesaurus Nal: | Genetic markers.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
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