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| Registros recuperados : 113 | |
| 21. |  | FREITAS, M. S.; NASCIMENTO, I. R. do; SÁ, C. O. de; SILVA, A. V. C. da. Efeito da qualidade do leite de cabra na rentabilidade da fabricação de queijo coalho Nossa Senhora da Glória, SE. Revista do Instituto de Laticínios Cândido Tostes, Juiz de Fora, v. 61, n. 351, p. 331-334, jul./ago., 2006.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 26. | | GRANGEIRO, L. C.; SOARES, J. M.; REIS, C. dos S.; DI GALVANINI, F.; SILVA, A. V. C. da. Armazenamento refrigerado de uva, cultivar Superior Seedless produzida no Vale do São Francisco. In: CONGRESSO BRASILEIRO DE FRUTICULTURA., 17., 2002, Belém, PA. Anais... Belém, PA: SBF, 2002. 1 CD-ROM.| Biblioteca(s): Embrapa Semiárido. |
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| 27. | | BARBIERI, R. L.; SILVA, A. V. C. da; MARCHI, M. M.; SOSINSKI JUNIOR, E. E.; SILVA JUNIOR, J. F. da. Ações de conservação in situ de fruteiras nativas. In: SILVA JUNIOR, J. F. da; SOUZA, F. V. D.; PADUA, J. G. (ed.). A arca de Noé das frutas nativas brasileiras. Brasília, DF: Embrapa, 2021. cap. 20, p. 198-205.| Biblioteca(s): Embrapa Clima Temperado; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
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| 29. | | SANTOS, G. M.; SILVA, A. V. C. da; OLIVEIRA, S. N.; YAGUIU, P.; CARNELOSSI, M. A. G.; MUNIZ, E. N. Injúria pelo frio em abóbora minimamente processada In: CONGRESSO BRASILEIRO DE OLERICULTURA, 47.; SIMPÓSIO BRASILEIRO SOBRE CUCURBITÁCIEAS, 4., 2007, Porto Seguro. Resgatando e valorizando as hortaliças subutilizadas. Porto Seguro: Associação Brasileira de Horticultura, 2007.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 33. | | CARVALHO, A. M. de; SOUZA, J. L.; SOUZA, S. F. de; MUNIZ, E. N.; SILVA, A. V. C. da. Estabelecimento e gestão do banco de germoplasma da Gliricidia sepium (BAG-Gliricidia) na Embrapa Tabuleiros Costeiros. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA TABULEIROS COSTEIROS, 12., 2023, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros, 2023. Editores Técnicos: Viviane Talamini, Ana da Silva Ledo.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 35. | | CARDOSO, E. C.; TEIXEIRA NETO, J. F.; VEIGA, J. B. da; FALESI, I. C.; SILVA, A. V. C. Contribution to the study of calcium and phosphorus on buffaloes in Marajo Island, Brazil. In: WORLD BUFFALO CONGRESS, 4., 1994, São Paulo. Proceedings... [S.l.: s.n., 1994?]. v. 2, p. 215-217.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 36. | | SILVA, A. V. C.; SANTOS, J. M.; MÔRO, F. V.; ARAÚJO NETO, J. C.; NÓBREGA, A. M. F. Caracterização dos grãos de polén da embaúba (Cecropia pachystachya, Trec) através da microscopia eletrônica de varredura. In: CONGRESSO E EXPOSIÇÃO INTERNACIONAL SOBRE FLORESTAS, 6., 2000, Porto Seguro. Resumos técnicos. Rio de Janeiro: Instituto Ambiental Biosfera, 2000. p.53.| Biblioteca(s): Embrapa Florestas. |
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| 39. | | GRILLI, G. V. G.; BRAZ, L. T.; LEMOS, E. G. M.; SILVA, A. V. C. da; DALPIAN, T. Avaliação de metodologia de extração de DNA genômico em tomateiros para análise genética com marcadores RAPD. Horticultura Brasileira, Brasília, v. 20, n. 2, p. 377, jul. 2002. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 40. | | FEITOSA, R. B.; SILVA, A. V. C. da; ALMEIDA, C. S.; SANTOS, A. R. F. dos; RANGEL, M. S. A. Caracterização molecular de genótipos de moringa. FEITOSA, R. B.; SILVA, A. V. C. da; ALMEIDA, C. S.; SANTOS, A. R. F. dos; RANGEL, M. S. A. Caracterização molecular de genótipos de moringa. In: SILVA, G. F. da; BERGAMASCO, R.; SERAFINI, M. R.; SANT?ANNA, M. C. S. (Org.). Potencialidades da Moringa: Oleifera Lam. São Cristóvão: Editora UFS, 2012. p. 27-40. il.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| Registros recuperados : 113 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
19/12/2023 |
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Data da última atualização: |
29/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
SANTOS, K. S.; COSTA, C.; BESSA, M. J.; TEIXEIRA, J. P.; SILVA, A. V. C. da; PADILHA, F. F.; DARIVA, C.; OLIVEIRA, M. B. P. P. |
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Afiliação: |
KLEBSON SILVA SANTOS, ITP; CARLA COSTA, Portuguese National Institute of Health; MARIA JOÃO BESSA, Universidade do Porto; JOAO PAULO TEIXEIRA, UNIVERSIDADE DO PORTO; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FRANCINE FERREIRA PADILHA, ITP; CLÁUDIO DARIVA, ITP; MARIA BEATRIZ PINTO PRIOR OLIVEIRA, UNIVERSIDADE DO PORTO. |
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Título: |
Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Exploration of Foods and Foodomics, v. 1, p. 130-142, 2023. |
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DOI: |
https://doi.org/10.37349/eff.2023.00011 |
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Idioma: |
Inglês |
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Conteúdo: |
Aim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. MenosAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negati... Mostrar Tudo |
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Thesagro: |
Conservação; Nim; Planta Medicinal. |
|
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02468naa a2200253 a 4500
001 2160010
005 2023-12-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.37349/eff.2023.00011$2DOI
100 1 $aSANTOS, K. S.
245 $aAzadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage.$h[electronic resource]
260 $c2023
520 $aAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells.
650 $aConservação
650 $aNim
650 $aPlanta Medicinal
700 1 $aCOSTA, C.
700 1 $aBESSA, M. J.
700 1 $aTEIXEIRA, J. P.
700 1 $aSILVA, A. V. C. da
700 1 $aPADILHA, F. F.
700 1 $aDARIVA, C.
700 1 $aOLIVEIRA, M. B. P. P.
773 $tExploration of Foods and Foodomics$gv. 1, p. 130-142, 2023.
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