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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
21/06/2018 |
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Data da última atualização: |
25/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, C. P. de; LIMA, R. M. B. de; NEVES, E. J. M.; GASPAROTTO, L.; DUNISCH, O. |
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Afiliação: |
CELSO PAULO DE AZEVEDO, CPAA; ROBERVAL MONTEIRO BEZERRA DE LIMA, CPAA; LUADIR GASPAROTTO, CPAA. |
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Título: |
Development of tree height and diameter of eight selected tree species under plantation conditions in Central Amazonia. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Mitteilungen der Bundesforschungsanstalt fur Forst und Holzwirtschaft, Hamburg, n. 193, p. 17-28, fev. 1999. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Amazon; Growth. |
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Thesagro: |
Árvore; Crescimento. |
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Thesaurus Nal: |
Amazonia; trees. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178959/1/Doc.1-Celso.pdf
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Marc: |
LEADER 00731naa a2200229 a 4500
001 2092689
005 2018-06-25
008 1999 bl --- 0-- u #d
100 1 $aAZEVEDO, C. P. de
245 $aDevelopment of tree height and diameter of eight selected tree species under plantation conditions in Central Amazonia.$h[electronic resource]
260 $c1999
650 $aAmazonia
650 $atrees
650 $aÁrvore
650 $aCrescimento
653 $aAmazon
653 $aGrowth
700 1 $aLIMA, R. M. B. de
700 1 $aNEVES, E. J. M.
700 1 $aGASPAROTTO, L.
700 1 $aDUNISCH, O.
773 $tMitteilungen der Bundesforschungsanstalt fur Forst und Holzwirtschaft, Hamburg$gn. 193, p. 17-28, fev. 1999.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/11/2022 |
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Data da última atualização: |
04/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MASCARIN, G. M.; SILVA, A. V. R. da; SILVA, T. P. da; KOBORI, N. N.; MORANDI, M. A. B.; BETTIOL, W. |
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Afiliação: |
GABRIEL MOURA MASCARIN, CNPMA; ANA VITÓRIA REINA DA SILVA; THIAGO PEREIRA DA SILVA; NILCE NAOMI KOBORI; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; WAGNER BETTIOL, CNPMA. |
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Título: |
Clonostachys rosea: production by submerged culture and bioactivity against Sclerotinia sclerotiorum and Bemisia tabaci. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Microbiology, v. 13, article 851000, 2022. |
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ISSN: |
1664-302 |
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DOI: |
https://doi.org/10.3389/fmicb.2022.851000 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Among the prospective biocontrol agents, the saprophytic filamentous fungus Clonostachys rosea is an excellent necrotrophic mycoparasite of numerous plant pathogenic fungi. However, its commercial development has been hampered by mass production difficulties during solid-state fermentation. Conversely, the submerged liquid fermentation shortens the cultivation time while increasing yields of fungal propagules. However, this method has been overlooked for C. rosea. In this work, we investigated the impact of liquid pre-culture inoculum on the spore production by the two-stage fermentation process using rice grains in comparison to the traditional solid-state fermentation. In parallel, we studied the submerged cultivation of C. rosea by manipulating carbon-to-nitrogen (C:N) ratio and nitrogen source, with the further optimization of spore production in a benchtop bioreactor. Additional bioassays included assessing the bioactivity of water-dispersible microgranules (that contained a submerged conidia) against the whitefly (Bemisia tabaci biotype B) and Sclerotinia sclerotiorum (causal agent of the white mold). Our results showed a maximum concentration of 1.1 × 109 conidia/g-dry-matter after 7 days of cultivation by two-stage fermentation process. The liquid fermentation yielded 1.4 × 109 submerged conidia/ml after 7 days using a medium with a 50:1 C:N ratio, and it also induced the production of microsclerotia (MS) up to 1.35 × 104/ml within 6 days with 10:1 C:N ratio; both media were supplemented with dextrose monohydrate and soybean meal. The fermentation batches carried out in a benchtop bioreactor with medium 50:1 C:N ratio and amended with soybean meal rendered a production peak on the fourth day, corresponding to 1.11 × 109 conidia/ml and 4.35 × 108 colony forming units (CFU)/ml. Following air-drying, the conidia production from air-dried microgranules of C. rosea biomass was estimated at 3.4 × 1010 conidia/g of formulated product upon re-hydration for 7 days. Both submerged conidia and MS of C. rosea inhibited 100% germination of S. sclerotiorum sclerotia by direct parasitism. The air-dried submerged conidia exhibited a suppressive activity on sclerotia (88% mycoparasitism) and early whitefly nymphs (76.2% mortality) that rendered LC50 values of 3.2 × 104 CFU/g soil and 1.5 × 107 CFU/ml, respectively. Therefore, the submerged liquid culture of C. rosea may offer a feasible and cost-effective method for its large-scale production, alleviating critical constraints to their commercial use while providing an additional tool for management of B. tabaci and S. sclerotiorum. MenosAbstract: Among the prospective biocontrol agents, the saprophytic filamentous fungus Clonostachys rosea is an excellent necrotrophic mycoparasite of numerous plant pathogenic fungi. However, its commercial development has been hampered by mass production difficulties during solid-state fermentation. Conversely, the submerged liquid fermentation shortens the cultivation time while increasing yields of fungal propagules. However, this method has been overlooked for C. rosea. In this work, we investigated the impact of liquid pre-culture inoculum on the spore production by the two-stage fermentation process using rice grains in comparison to the traditional solid-state fermentation. In parallel, we studied the submerged cultivation of C. rosea by manipulating carbon-to-nitrogen (C:N) ratio and nitrogen source, with the further optimization of spore production in a benchtop bioreactor. Additional bioassays included assessing the bioactivity of water-dispersible microgranules (that contained a submerged conidia) against the whitefly (Bemisia tabaci biotype B) and Sclerotinia sclerotiorum (causal agent of the white mold). Our results showed a maximum concentration of 1.1 × 109 conidia/g-dry-matter after 7 days of cultivation by two-stage fermentation process. The liquid fermentation yielded 1.4 × 109 submerged conidia/ml after 7 days using a medium with a 50:1 C:N ratio, and it also induced the production of microsclerotia (MS) up to 1.35 × 104/ml... Mostrar Tudo |
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Thesagro: |
Bemisia Tabaci; Controle Biológico; Doença de Planta; Fungo Para Controle Biológico; Mofo Branco; Sclerotinia Sclerotiorum. |
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Thesaurus NAL: |
Biological control agents; Clonostachys rosea; Sclerotinia; Solid state fermentation; Submerged fermentation. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148058/1/Mascarin-Clonostachys-rosea-2022.pdf
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Marc: |
LEADER 03881naa a2200337 a 4500
001 2148058
005 2022-11-04
008 2022 bl uuuu u00u1 u #d
022 $a1664-302
024 7 $ahttps://doi.org/10.3389/fmicb.2022.851000$2DOI
100 1 $aMASCARIN, G. M.
245 $aClonostachys rosea$bproduction by submerged culture and bioactivity against Sclerotinia sclerotiorum and Bemisia tabaci.$h[electronic resource]
260 $c2022
520 $aAbstract: Among the prospective biocontrol agents, the saprophytic filamentous fungus Clonostachys rosea is an excellent necrotrophic mycoparasite of numerous plant pathogenic fungi. However, its commercial development has been hampered by mass production difficulties during solid-state fermentation. Conversely, the submerged liquid fermentation shortens the cultivation time while increasing yields of fungal propagules. However, this method has been overlooked for C. rosea. In this work, we investigated the impact of liquid pre-culture inoculum on the spore production by the two-stage fermentation process using rice grains in comparison to the traditional solid-state fermentation. In parallel, we studied the submerged cultivation of C. rosea by manipulating carbon-to-nitrogen (C:N) ratio and nitrogen source, with the further optimization of spore production in a benchtop bioreactor. Additional bioassays included assessing the bioactivity of water-dispersible microgranules (that contained a submerged conidia) against the whitefly (Bemisia tabaci biotype B) and Sclerotinia sclerotiorum (causal agent of the white mold). Our results showed a maximum concentration of 1.1 × 10<sup>9</sup> conidia/g-dry-matter after 7 days of cultivation by two-stage fermentation process. The liquid fermentation yielded 1.4 × 10<sup>9</sup> submerged conidia/ml after 7 days using a medium with a 50:1 C:N ratio, and it also induced the production of microsclerotia (MS) up to 1.35 × 10<sup>4</sup>/ml within 6 days with 10:1 C:N ratio; both media were supplemented with dextrose monohydrate and soybean meal. The fermentation batches carried out in a benchtop bioreactor with medium 50:1 C:N ratio and amended with soybean meal rendered a production peak on the fourth day, corresponding to 1.11 × 10<sup>9</sup> conidia/ml and 4.35 × 10<sup>8</sup> colony forming units (CFU)/ml. Following air-drying, the conidia production from air-dried microgranules of C. rosea biomass was estimated at 3.4 × 10<sup>10</sup> conidia/g of formulated product upon re-hydration for 7 days. Both submerged conidia and MS of C. rosea inhibited 100% germination of S. sclerotiorum sclerotia by direct parasitism. The air-dried submerged conidia exhibited a suppressive activity on sclerotia (88% mycoparasitism) and early whitefly nymphs (76.2% mortality) that rendered LC<sub>50</sub> values of 3.2 × 10<sup>4</sup> CFU/g soil and 1.5 × 10<sup>7</sup> CFU/ml, respectively. Therefore, the submerged liquid culture of C. rosea may offer a feasible and cost-effective method for its large-scale production, alleviating critical constraints to their commercial use while providing an additional tool for management of B. tabaci and S. sclerotiorum.
650 $aBiological control agents
650 $aClonostachys rosea
650 $aSclerotinia
650 $aSolid state fermentation
650 $aSubmerged fermentation
650 $aBemisia Tabaci
650 $aControle Biológico
650 $aDoença de Planta
650 $aFungo Para Controle Biológico
650 $aMofo Branco
650 $aSclerotinia Sclerotiorum
700 1 $aSILVA, A. V. R. da
700 1 $aSILVA, T. P. da
700 1 $aKOBORI, N. N.
700 1 $aMORANDI, M. A. B.
700 1 $aBETTIOL, W.
773 $tFrontiers in Microbiology$gv. 13, article 851000, 2022.
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Embrapa Meio Ambiente (CNPMA) |
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