Data/hora: 18/06/2024 02:39:40

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 15/10/2010
Data da última atualização: | 30/04/2018
Autoria: | DROSTE, A.; SILVA, A. M. da; SOUZA, I. F. de; WIEBKE-STROHM, B.; BÜCKER-NETO, L.; SAUNER, M. V.; BODANESE-ZANETTINI, M. H.
Afiliação: | Annette Droste, Universidade Feevale; Anelise Machado da Silva, Universidade Federal do Rio Grande do Sul; Iliane Freitas de Souza, Universidade do Vale do Rio dos Sinos; Beatriz Wiebke-Strohm, Universidade Federal do Rio Grande do Sul; Lauro Bücker-Neto, Universidade Federal do Rio Grande do Sul; Márcia Viviane Sauner, Universidade do Vale do Rio dos Sinos; Maria Helena Bodanese-Zanettini, Universidade Federal do Rio Grande do Sul.
Título: | Screening of Brazilian soybean genotypes with high potential for somatic embryogenesis and plant regeneration.
Ano de publicação: | 2010
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 7, p. 715-720, jul. 2010
Idioma: | Inglês
Notas: | Título em português: Seleção de genótipos brasileiros de soja com alto potencial para embriogênese somática e regeneração de plantas.
Conteúdo: | The aim of this work was to identify Brazilian soybean (Glycine max) genotypes with potential to respond to in vitro culture stimuli for primary somatic embryo induction, secondary embryo proliferation and plant regeneration. Differences among eight tested cultivars were observed at each stage. Two cultivars, IAS-5 and BRSMG 68 Vencedora, were selected for the evaluation of the capacity for embryo differentiation and plant regeneration. These cultivars had high embryo induction frequencies, repetitive embryogenic proliferation, and low precocious embryo germination in the initial experiment. The effect of abscisic acid (ABA) and charcoal addition on plant regeneration was investigated. The addition of ABA to proliferation medium and of ABA and activated charcoal to maturation medium increased embryo differentiation rates, which resulted in a higher number of regenerated plants. The BRSMG 68 Vencedora cultivar was found to have a high potential for embryo induction, embryo proliferation and plant regeneration. The potential of this cultivar for somatic embryogenesis was similar to that observed for cultivar IAS-5, which is currently used for soybean transformation in Brazil. BRSMG 68 Vencedora may be a good alternative genotype for soybean genetic engineering via somatic embryogenesis protocols.
Palavras-Chave: | Brazilian cultivars; Cultivar brasileira; Embryogenic potential; Potencial embriogênico; Soybean transformation; Transformação de soja.
Thesagro: | Engenharia genética; Glycine Max.
Thesaurus Nal: | Genetic engineering.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/105835/1/Screening.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (48304-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS