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| 3. |  | LIMA, R. O. de; SILVA, L. M. da; SILVA, A. L. D. da; CAMPOS, T. de. Identificação genética de híbridos interespecíficos de Arachis. In: SEMINÁRIO DA EMBRAPA ACRE DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO, 3., 2020, Rio Branco, AC. Ciência e tecnologia na sociedade digital (edição on-line): anais. Rio Branco, AC: Embrapa Acre, 2021. p. 59-64. Apresentação oral. (Embrapa Acre. Eventos técnicos & científicos, 3). Editores: Virgínia de Souza Álvares, Fabiano Marçal Estanislau.| Biblioteca(s): Embrapa Acre. |
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| 4. | | SILVA, A. L. D. da; OLIVEIRA, J. C. de; CAMPOS, T. de. Identificação molecular de clones de seringueira com marcadores microssatélites. In: SEMINÁRIO DA EMBRAPA ACRE DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO, 2., 2019, Rio Branco, AC. A Contribuição da ciência para a agropecuária no Acre: anais. Rio Branco, AC: Embrapa Acre, 2020. p. 111-116. Banner. (Embrapa Acre. Eventos técnicos & científicos, 2). Editores técnicos: Virgínia de Souza Álvares; Fabiano Marçal Estanislau.| Biblioteca(s): Embrapa Acre. |
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| 5. | | ALMEIDA, R. L. de; MACEDO, F. S.; SOUSA, G. V. de; SILVA, A. L. D. da; DRUMOND, P. M. Outros aspectos regulatórios. In: DRUMOND, P. M.; CARVALHO-ZILSE, G. A.; WITTER, S.; ALVES, R. M. de O.; DRUMMOND, M. S. (ed.). Meliponicultura: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa, 2024. cap. 20. p. 213-222. (Coleção 500 perguntas, 500 respostas).| Biblioteca(s): Embrapa Meio-Norte; Embrapa Unidades Centrais. |
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| 6. | | SILVA, A. L. D. da; ALVES FILHO, E. G.; SILVA, L. M. A. e; TAVARES, O. C. H.; PEREIRA, M. G.; CAMPOS, T. de; SILVA, L. M. da. Near infrared spectroscopy to rapid assess the rubber tree clone and the influence of maturation and disease at the leaves. Microchemical Journal, v. 168, 106478, Sept. 2021.| Biblioteca(s): Embrapa Acre; Embrapa Agroindústria Tropical. |
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| 7. | | OLIVEIRA, J. C. de; SILVA, A. L. D. da; SILVA, C. C. da; SOUZA, A. P. de; FORMIGHIERI, E. F.; CAMPOS, T. de. Caracterização de bibliotecas de RNA-Seq de amendoim forrageiro para o desenvolvimento de novos microssatélites. In: SEMINÁRIO DA EMBRAPA ACRE DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO, 2., 2019, Rio Branco, AC. A Contribuição da ciência para a agropecuária no Acre: anais. Rio Branco, AC: Embrapa Acre, 2020. p. 83-88. Banner. (Embrapa Acre. Eventos técnicos & científicos, 2). Editores técnicos: Virgínia de Souza Álvares; Fabiano Marçal Estanislau.| Biblioteca(s): Embrapa Acre. |
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| 8. | | OLIVEIRA, J. C. de; SILVA, A. L. D. da; SILVA, C. C. da; SOUZA, A. P. de; FORMIGHIERI, E. F.; SILVA, L. M. da; CAMPOS, T. de. Transferibilidade de marcadores microssatélites desenvolvidos a partir de RNA-Seq de amendoim forrageiro. In: SEMINÁRIO DA EMBRAPA ACRE DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO, 3., 2020, Rio Branco, AC. Ciência e tecnologia na sociedade digital (edição on-line): anais. Rio Branco, AC: Embrapa Acre, 2021. p. 113-117. Banner. (Embrapa Acre. Eventos técnicos & científicos, 3). Editores: Virgínia de Souza Álvares, Fabiano Marçal Estanislau.| Biblioteca(s): Embrapa Acre. |
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| 9. | | OLIVEIRA, J. C. de; SILVA, A. L. D. da; SILVA, L. M. da; FORMIGHIERI, E. F.; PETERS, L. P.; ASSIS, G. M. L. de; SILVA, C. C. da; SOUZA, A. P. DE; CAMPOS, T. de. Novel microsatellite markers derived from Arachis pintoi transcriptome sequencing for cross?species transferability and varietal identification. Plant Molecular Biology Reporter, v. 41, n. 4, 2023.| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | OLIVEIRA, J. C. de; SILVA, A. L. D. da; SILVA, L. M. da; FORMIGHIERI, E. F.; PETERS, L. P.; ASSIS, G. M. L. de; SILVA, C. C. da; SOUZA, A. P. de; CAMPOS, T. de. Novel microsatellite markers derived from Arachis pintoi transcriptome sequencing for cross-species transferability and varietal identification. Plant Molecular Biology Reporter, v. 42, p. 183-192, Mar. 2024.| Biblioteca(s): Embrapa Acre. |
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| Registros recuperados : 10 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
19/10/2023 |
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Data da última atualização: |
26/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 3 |
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Autoria: |
OLIVEIRA, J. C. de; SILVA, A. L. D. da; SILVA, L. M. da; FORMIGHIERI, E. F.; PETERS, L. P.; ASSIS, G. M. L. de; SILVA, C. C. da; SOUZA, A. P. DE; CAMPOS, T. de. |
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Afiliação: |
JÔNATAS CHAGAS DE OLIVEIRA, UNIVERSIDADE FEDERAL DO ACRE; ANDRÉ LUCAS DOMINGOS DA SILVA, UNIVERSIDADE FEDERAL DO ACRE; LUCIELIO MANOEL DA SILVA, CPATC; EDUARDO FERNANDES FORMIGHIERI, CNPAE; LEILA PRISCILA PETERS, UNIVERSIDADE FEDERAL DO ACRE; GISELLE MARIANO LESSA DE ASSIS, CPAF-AC; CARLA CRISTINA DA SILVA, UNIVERSIDADE DE CAMPINAS; ANETE PEREIRA DE SOUZA, UNIVERSITY OF CAMPINAS; TATIANA DE CAMPOS, CPAF-AC. |
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Título: |
Novel microsatellite markers derived from Arachis pintoi transcriptome sequencing for cross?species transferability and varietal identification. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 41, n. 4, 2023. |
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DOI: |
https://doi.org/10.1007/s11105-023-01402-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Forage peanut (Arachis pintoi) is an important leguminous forage that has gained popularity due to increased livestock productivity. Furthermore, the species helps with soil fertility and the restoration of degraded areas. However, A. pintoi has a limited number of molecular markers. The objective of this study was to create and characterize gene-derived microsatellite markers as well as to test their transferability to the peanut (Arachis hypogaea) and other six wild Arachis species. A total of 4461 putative simple sequence repeats (SSR) were identified, and PCR primer pairs were designed for 999 SSR regions after filtering out primers with the same annealing site and searching for sequences related to open reading frames (ORFs). The dinucleotide motif was the most common (628; 62.86%). For validation, 186 primer pairs were chosen at random, of which 63 (33.87%) were polymorphic, with an average of 7.37 alleles per locus. Polymorphic information content (PIC = 0.70) and discriminatory power (D = 0.80) were both high on average. The functional annotation discovered 120 sequences that were assigned to 87 gene ontology functional groups divided into three main categories: molecular function (27 sub-categories), cellular components (21 sub-categories), and biological process (39 sub-categories). Thirty-three SSRs were tested for transferability to peanut and six other wild Arachis species, resulting in variable cross-species amplification (63.64 to 100%). Here, we present the first gene-derived SSR for A. pintoi. These new informative microsatellites may be linked to agronomically important genes to be used in genetic studies. MenosForage peanut (Arachis pintoi) is an important leguminous forage that has gained popularity due to increased livestock productivity. Furthermore, the species helps with soil fertility and the restoration of degraded areas. However, A. pintoi has a limited number of molecular markers. The objective of this study was to create and characterize gene-derived microsatellite markers as well as to test their transferability to the peanut (Arachis hypogaea) and other six wild Arachis species. A total of 4461 putative simple sequence repeats (SSR) were identified, and PCR primer pairs were designed for 999 SSR regions after filtering out primers with the same annealing site and searching for sequences related to open reading frames (ORFs). The dinucleotide motif was the most common (628; 62.86%). For validation, 186 primer pairs were chosen at random, of which 63 (33.87%) were polymorphic, with an average of 7.37 alleles per locus. Polymorphic information content (PIC = 0.70) and discriminatory power (D = 0.80) were both high on average. The functional annotation discovered 120 sequences that were assigned to 87 gene ontology functional groups divided into three main categories: molecular function (27 sub-categories), cellular components (21 sub-categories), and biological process (39 sub-categories). Thirty-three SSRs were tested for transferability to peanut and six other wild Arachis species, resulting in variable cross-species amplification (63.64 to 100%). Here, we present the f... Mostrar Tudo |
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Thesaurus NAL: |
Arachis; Forage; Peanuts. |
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Categoria do assunto: |
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Marc: |
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100 1 $aOLIVEIRA, J. C. de
245 $aNovel microsatellite markers derived from Arachis pintoi transcriptome sequencing for cross?species transferability and varietal identification.$h[electronic resource]
260 $c2023
520 $aForage peanut (Arachis pintoi) is an important leguminous forage that has gained popularity due to increased livestock productivity. Furthermore, the species helps with soil fertility and the restoration of degraded areas. However, A. pintoi has a limited number of molecular markers. The objective of this study was to create and characterize gene-derived microsatellite markers as well as to test their transferability to the peanut (Arachis hypogaea) and other six wild Arachis species. A total of 4461 putative simple sequence repeats (SSR) were identified, and PCR primer pairs were designed for 999 SSR regions after filtering out primers with the same annealing site and searching for sequences related to open reading frames (ORFs). The dinucleotide motif was the most common (628; 62.86%). For validation, 186 primer pairs were chosen at random, of which 63 (33.87%) were polymorphic, with an average of 7.37 alleles per locus. Polymorphic information content (PIC = 0.70) and discriminatory power (D = 0.80) were both high on average. The functional annotation discovered 120 sequences that were assigned to 87 gene ontology functional groups divided into three main categories: molecular function (27 sub-categories), cellular components (21 sub-categories), and biological process (39 sub-categories). Thirty-three SSRs were tested for transferability to peanut and six other wild Arachis species, resulting in variable cross-species amplification (63.64 to 100%). Here, we present the first gene-derived SSR for A. pintoi. These new informative microsatellites may be linked to agronomically important genes to be used in genetic studies.
650 $aArachis
650 $aForage
650 $aPeanuts
700 1 $aSILVA, A. L. D. da
700 1 $aSILVA, L. M. da
700 1 $aFORMIGHIERI, E. F.
700 1 $aPETERS, L. P.
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700 1 $aSILVA, C. C. da
700 1 $aSOUZA, A. P. DE
700 1 $aCAMPOS, T. de
773 $tPlant Molecular Biology Reporter$gv. 41, n. 4, 2023.
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