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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
21/02/2019 |
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Data da última atualização: |
13/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. G. da; MARTINS, C. F.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; CARDOSO, T. C.; FRANCO, M. M.; MENDONÇA, A. dos S.; LEME, L. de O.; BORGES, J. R. J.; MALAQUIAS, J. V.; BAO, S. N. |
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Afiliação: |
CAROLINA GONZALES DA SILVA, UNB; CARLOS FREDERICO MARTINS, CPAC; HEIDI CHRISTINA BESSLER CUMPA, CPAC; ALVARO MORAES DA FONSECA NETO, CPAC; TEREZA CRISTINA CARDOSO, UNESP; MAURICIO MACHAIM FRANCO, Cenargen; ANELISE DOS SANTOS MENDONÇA, UNIVERSIDADE DE UBERLÂNDIA; LIGIANE DE OLIVEIRA LEME, Cenargen; JOSÉ RENATO JUNQUEIRA BORGES, UNB; JUACI VITORIA MALAQUIAS, CPAC; SONIA NAIR BAO, UNB. |
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Título: |
Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 54, n. 2, p. 289-299, February 2019. |
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DOI: |
10.1111/rda.13360 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce lower expression of HDAC3 and higher expression of KAT2A in the embryos and consequently improve bovine embryo production. MenosAbstract: The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce l... Mostrar Tudo |
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Palavras-Chave: |
Acetilação de histona; Célula adiposa; Célula amniótica. |
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Thesagro: |
Bovino; Clonagem; DNA; Melhoramento Genético Animal; Metilação. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02722naa a2200349 a 4500
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008 2019 bl uuuu u00u1 u #d
024 7 $a10.1111/rda.13360$2DOI
100 1 $aSILVA, C. G. da
245 $aUse of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells.$h[electronic resource]
260 $c2019
520 $aAbstract: The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce lower expression of HDAC3 and higher expression of KAT2A in the embryos and consequently improve bovine embryo production.
650 $aBovino
650 $aClonagem
650 $aDNA
650 $aMelhoramento Genético Animal
650 $aMetilação
653 $aAcetilação de histona
653 $aCélula adiposa
653 $aCélula amniótica
700 1 $aMARTINS, C. F.
700 1 $aCUMPA, H. C. B.
700 1 $aFONSECA NETO, A. M. da
700 1 $aCARDOSO, T. C.
700 1 $aFRANCO, M. M.
700 1 $aMENDONÇA, A. dos S.
700 1 $aLEME, L. de O.
700 1 $aBORGES, J. R. J.
700 1 $aMALAQUIAS, J. V.
700 1 $aBAO, S. N.
773 $tReproduction in Domestic Animals$gv. 54, n. 2, p. 289-299, February 2019.
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| Registros recuperados : 70 | |
| 61. |  | SILVA, A. K.; MARTINS, A. C. Q.; MORGANTE, C. V.; ARAUJO, A. C. G. de; BERTIOLI, S. C. de M. L.; BERTIOLI, D. J.; GUIMARAES, P. M.; BRASILEIRO, A. C. M. Large scale transcriptome analysis of wild peanut (Arachis duranensis) under gradual water stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 82.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 62. | | BRASILEIRO, A. C. M.; MORGANTE, C. V.; SILVA, A. K.; MARTINS, A. C. Q.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M. Next Generation Sequencing (NGS) for discovery of droughtresponsive genes in wild Arachis. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 63. | | BRASILEIRO, A. C. M.; MORGANTE, C. V.; SILVA, A. K.; MARTINS, A. C. Q.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M. Next Generation Sequencing (NGS) for discovery of droughtresponsive genes in wild Arachis. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 64. | | SILVA, A. K. L.; SEGNINI, A.; MILORI, D. M. B. P.; OLIVEIRA, M. E. de; MARTORANO, L. G.; VASCONCELOS, S. S.; SOUZA, A. M. L. de; RODRIGUES, S. J. S. C.; NUNES, H. G. G. C.; FACHINI, I. A.; CRESTANA, S. Estoque de carbono e cálculo do índice de humidificação por espectroscopia de fluorescência induzida em áreas com palma de óleo: coleta, processamento e análise do solo. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2014. p. 601-604. SIAGRO 2014.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 65. | | SILVA, A. K. L.; SEGNINI, A.; MILORI, D. M. B. P.; OLIVEIRA, M. E. de; MARTORANO, L. G.; VASCONCELOS, S. S.; SOUZA, A. M. L. de; RODRIGUES, S. J. S. C.; NUNES, H. G. G. C.; FACHINI, I. A.; CRESTANA, S. Estoque de carbono e cálculo do índice de humificação por epectroscopia de fluorescência induzida em áreas com palma de óleo: coleta, processamento e análise do solo. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 601-604. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 66. | | MARTINS, A. C. Q.; MORGANTE, C. V.; SILVA, A. K.; GALHARDO, I. C.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; BERTIOLI, D. J.; MILLER, R. N. G.; BRASILEIRO, A. C. M.; GUIMARAES, P. M. Large scale transcriptome analysis of wild peanut (Arachis stenosperma) inoculated with Passalora personata, the causal agent of late leaf spot. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 76.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 67. | | MARTINS, A. C. Q.; MORGANTE, C. V.; SILVA, A. K.; GALHARDO, I. C.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; BERTIOLI, D. J.; MILLER, R. N. G.; BRASILEIRO, A. C. M.; GUIMARAES, P. M. Large scale transcriptome analysis of wild peanut (Arachis stenosperma) inoculated with Passalora personata, the causal agent of late leaf spot. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 76.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 68. | | MATOS, T. S.; SILVA, A. K. O.; QUINTELA, A. L.; PINTO, F. das C.; CANUTO, K. M.; BRAZ-FILHO, R.; FONSECA, M. J. da; LUNA-COSTA, A. M.; PAZ, I. A.; NASCIMENTO, N. R. F. do; SILVEIRA, E. R.; PESSOA, O. D. L. Neuroinhibitory meroterpenoid compounds from Cordia oncocalyx. Fitoterapia, v. 123, p. 65-72, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 69. | | BRASILEIRO, A. C. M.; MORGANTE, C. V.; ARAUJO, A. C. G.; LEAL-BERTIOLI, S. C. M.; SILVA, A. K.; MARTINS, A. C. Q.; VINSON, C. C.; SANTOS, C. M. R.; BONFIM, O.; TOGAWA, R. C.; SARAIVA, M. A. P.; BERTIOLI, D. J.; GUIMARAES, P. M. Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes. Plant Molecular Biology Reporter, v. 33, p. 1876-1892, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 70. | | SANTOS, M. E. C. DOS; MELO, R. R. DE; CORREIA, D.; SOUSA, J. A. de; SANTOS, A. M.; SILVA, A. K. V. DA; PAULA, E. A. DE O.; ALVES, A. R.; SCATOLINO, M. V.; RUSCH, F.; MASCARENHAS, A. R. P.; PIMENTA, A. S.; STANGERLIN, D. M. Variation in the Basic Density of Woods Produced in the Brazilian Semiarid Region Subjected to Different Irrigation Regimes. Forests, 14, 2168, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Florestas. |
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| Registros recuperados : 70 | |
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