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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
17/12/2018 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, I. H. S. da; GOMÉZ, I.; SÁNCHEZ, J.; CASTRO, D. L. M. de; VALICENTE, F. H.; SOBERÓN, M.; POLANCZYK, R. A.; BRAVO, A. |
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Afiliação: |
Igor Henrique Sena da Silva, Universidade Estadual Paulista; Isabel Goméz, Universidad Nacional Autónoma de México; Jorge Sánchez, Universidad Nacional Autónoma de México; Diana L. Martínez de Castro, Universidad Nacional Autónoma de México; FERNANDO HERCOS VALICENTE, CNPMS; Mario Soberón, Universidad Nacional Autónoma de México; Ricardo Antonio Polanczyk, Universidade Estadual Paulista; Alejandra Bravo, Universidad Nacional Autónoma de México. |
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Título: |
Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plos One, San Francisco, v. 13, n. 12, e0207789, 2018. |
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DOI: |
10.1371/journal.pone.0207789 |
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Idioma: |
Inglês |
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Conteúdo: |
Helicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesting that increased proteolytic activity on the toxin could also explain the low Cry1Ac toxicity in late instars. MenosHelicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesti... Mostrar Tudo |
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Thesagro: |
Praga de Planta; Proteína; Toxina. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188667/1/Identification-midgut.pdf
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Marc: |
LEADER 02401naa a2200253 a 4500
001 2101792
005 2019-02-18
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0207789$2DOI
100 1 $aSILVA, I. H. S. da
245 $aIdentification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera$bNoctuidae) that bind to Cry1Ac toxin.$h[electronic resource]
260 $c2018
520 $aHelicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesting that increased proteolytic activity on the toxin could also explain the low Cry1Ac toxicity in late instars.
650 $aPraga de Planta
650 $aProteína
650 $aToxina
700 1 $aGOMÉZ, I.
700 1 $aSÁNCHEZ, J.
700 1 $aCASTRO, D. L. M. de
700 1 $aVALICENTE, F. H.
700 1 $aSOBERÓN, M.
700 1 $aPOLANCZYK, R. A.
700 1 $aBRAVO, A.
773 $tPlos One, San Francisco$gv. 13, n. 12, e0207789, 2018.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
03/05/2005 |
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Data da última atualização: |
16/08/2018 |
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Autoria: |
PRIMAVESI, A. C.; PRIMAVESI, O.; CORRÊA, L. A.; CANTARELLA, H.; SILVA, A. G. da. |
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Afiliação: |
Ana Cândida Primavesi, CPPSE; Odo Primavesi, CPPSE; Luciano Almeida Corrêa, CPPSE; Heitor Cantarella, Instituto Agronômico de Campinas - IAC/Centro de Solos e Recursos Agroambientais; Aliomar Gabriel da Silva, CPPSE. |
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Título: |
Absorção de cátions e ânions pelo capim-coastcross adubado com uréia e nitrato de amônio. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 3, p. 247-253, mar. 2005 |
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Idioma: |
Português |
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Notas: |
Título em inglês: Cations and anions uptake by coastcross grass fertilized with urea and ammonium nitrate. |
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Conteúdo: |
Os processos fisiológicos das plantas são afetados pelo balanço de cátions e ânions absorvidos. O objetivo deste trabalho foi determinar a absorção de cátions e ânions quando plantas de capim-coastcross receberam doses elevadas de nitrogênio. O delineamento experimental foi o de blocos casualizados com quatro repetições, num esquema fatorial 2x5 - duas fontes de N: uréia e nitrato de amônio, e cinco doses de N: 0, 25, 50, 100, 200 kg ha-1 corte-1. As doses de N foram aplicadas após cada corte num total de cinco cortes, durante a época das chuvas. A absorção de cátions e de ânions pelo capim-coastcross aumentou com o acréscimo das doses de N dos dois fertilizantes, sendo maior com o nitrato de amônio. Com doses crescentes de N, verificou-se entre os cátions maior absorção do K+, e do Cl- entre os ânions. Com exceção do N, a absorção do K+ foi superior à dos demais nutrientes, com redução no teor relativo de cálcio. Doses altas de N aplicadas em capim-coastcross, na forma de uréia ou de nitrato de amônio, favorecem a absorção de cátions e de ânions. |
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Palavras-Chave: |
adubos nitrogenados; nutrients absorption. |
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Thesagro: |
Absorção de Nutrientes; Cynodon Dactylon. |
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Thesaurus NAL: |
nitrogen fertilizers. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/30531/1/40n03a08.pdf
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Marc: |
LEADER 01942naa a2200241 a 4500
001 1113877
005 2018-08-16
008 2005 bl uuuu u00u1 u #d
100 1 $aPRIMAVESI, A. C.
245 $aAbsorção de cátions e ânions pelo capim-coastcross adubado com uréia e nitrato de amônio.
260 $c2005
500 $aTítulo em inglês: Cations and anions uptake by coastcross grass fertilized with urea and ammonium nitrate.
520 $aOs processos fisiológicos das plantas são afetados pelo balanço de cátions e ânions absorvidos. O objetivo deste trabalho foi determinar a absorção de cátions e ânions quando plantas de capim-coastcross receberam doses elevadas de nitrogênio. O delineamento experimental foi o de blocos casualizados com quatro repetições, num esquema fatorial 2x5 - duas fontes de N: uréia e nitrato de amônio, e cinco doses de N: 0, 25, 50, 100, 200 kg ha-1 corte-1. As doses de N foram aplicadas após cada corte num total de cinco cortes, durante a época das chuvas. A absorção de cátions e de ânions pelo capim-coastcross aumentou com o acréscimo das doses de N dos dois fertilizantes, sendo maior com o nitrato de amônio. Com doses crescentes de N, verificou-se entre os cátions maior absorção do K+, e do Cl- entre os ânions. Com exceção do N, a absorção do K+ foi superior à dos demais nutrientes, com redução no teor relativo de cálcio. Doses altas de N aplicadas em capim-coastcross, na forma de uréia ou de nitrato de amônio, favorecem a absorção de cátions e de ânions.
650 $anitrogen fertilizers
650 $aAbsorção de Nutrientes
650 $aCynodon Dactylon
653 $aadubos nitrogenados
653 $anutrients absorption
700 1 $aPRIMAVESI, O.
700 1 $aCORRÊA, L. A.
700 1 $aCANTARELLA, H.
700 1 $aSILVA, A. G. da
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 40, n. 3, p. 247-253, mar. 2005
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Embrapa Unidades Centrais (AI-SEDE) |
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