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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
18/03/2022 |
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Data da última atualização: |
18/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, J. C.; SOARES, A. C.; ANGELIM, M. K. S. C.; PROENÇA-MODENA, J. L.; VIEIRA, P. M. M.; MATTOSO, L. H. C.; OLIVEIRA JR, O. N. |
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Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
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Título: |
Diagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Talanta, v. 239, 123076, 2022. |
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Páginas: |
1 - 7 |
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ISSN: |
0039-9140 |
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DOI: |
https://doi.org/10.1016/j.talanta.2021.123076 |
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Idioma: |
Inglês |
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Conteúdo: |
Mass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. MenosMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be ap... Mostrar Tudo |
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Palavras-Chave: |
Impedance spectroscopy; Information visualization; SARS-CoV-2; Spike protein. |
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Categoria do assunto: |
-- |
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Marc: |
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022 $a0039-9140
024 7 $ahttps://doi.org/10.1016/j.talanta.2021.123076$2DOI
100 1 $aSOARES, J. C.
245 $aDiagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein.$h[electronic resource]
260 $c2022
300 $a1 - 7
520 $aMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources.
653 $aImpedance spectroscopy
653 $aInformation visualization
653 $aSARS-CoV-2
653 $aSpike protein
700 1 $aSOARES, A. C.
700 1 $aANGELIM, M. K. S. C.
700 1 $aPROENÇA-MODENA, J. L.
700 1 $aVIEIRA, P. M. M.
700 1 $aMATTOSO, L. H. C.
700 1 $aOLIVEIRA JR, O. N.
773 $tTalanta$gv. 239, 123076, 2022.
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| 1. |  | CARVALHO, H. W. L. de; CARDOSO, M. J.; TABOSA, J. N.; SILVA, A. A. G. e; LIRA, M. A.; GUIMARÃES, P. E. O.; PACHECO, C. A. P.; GAMA, E. E. G. da; ALBUQUERQUE, M. M. de; AMIM, S. M. F.; SOUZA, I. V. B.; BRITO, A. R. de M. B.; RODRIGUES, A. R. dos S.; SOUZA, E. M. de; RIBEIRO, S. S.; OLIVEIRA, V. D. de; RODRIGUES, K. F. Comportamento adaptabilidade e estabilidade de cultivares de milho no nordeste brasileiro no ano agrícola de 2004/2005. Aracaju: Embrapa Tabuleiros Costeiros, 2006. 21 p. (Embrapa Tabuleiros Costeiros. Boletim de Pesquisa e Desenvolvimento, 19).| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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