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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
30/04/2003 |
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Data da última atualização: |
13/03/2017 |
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Autoria: |
FIGUEIREDO, J. F.; PELLEGRIN, A. O.; FOSCOLO, C. B.; MACHADO, R. P.; MIRANDA, K. L.; LAGE, A. P. |
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Afiliação: |
UFMG/EV (Belo Horizonte, MG); EMBRAPA Pantanal (Corumba, MS). |
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Título: |
Evaluation of direct fluorescent antibody test for the diagnosis of Bovine Genital Campylobacteriosis. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Revista Latinoamericana de Microbiologia, v.44, n.3/4, p.118-123, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
The direct fluorescent antibody test (DFAT) for the diagnosis of Bovine Genital Campylo- bacteriosis was assessed for its detectgion limit, observer effect, sensitivity and specificity. In addition, the specificity of the fluorescent conjugate was tested against Campylobacter sp., Arcobacter sp., Helicobacter sp., E. coli and other bacteria from the preputial flora. Ten - fold dilutions of C. fetus subs. venerealis NCTC 10354 in PBS or preputial washing with or without centrifugation were used. All experiments were done in duplicate by three observers. Positive and negative controls were included in each assay. The detection li- mits of DFAT were 104 CFU/ml for PBS and non - centrifuged preputial washings and 102 CFU/ml for centrifuged preputial washing. There was no observer effect. The sensitivity and specificity of DFAT were 92.59% and 88.89%, respectively. The DFAT was observed to be sensitive, specific and the effect of experienced observers was minimal on test per- formance. |
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Palavras-Chave: |
Antibody test; Bovine genital campylobacteriosis; Campilobacteriosis genital bobina; Diagnosis; Direct fluorescent; Imunofluorescencia direta; Lavado prepucial; Preputial washing. |
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Thesagro: |
Campylobacter Fetus; Diagnostico. |
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Categoria do assunto: |
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Marc: |
LEADER 01915naa a2200301 a 4500
001 1810638
005 2017-03-13
008 2002 bl --- 0-- u #d
100 1 $aFIGUEIREDO, J. F.
245 $aEvaluation of direct fluorescent antibody test for the diagnosis of Bovine Genital Campylobacteriosis.
260 $c2002
520 $aThe direct fluorescent antibody test (DFAT) for the diagnosis of Bovine Genital Campylo- bacteriosis was assessed for its detectgion limit, observer effect, sensitivity and specificity. In addition, the specificity of the fluorescent conjugate was tested against Campylobacter sp., Arcobacter sp., Helicobacter sp., E. coli and other bacteria from the preputial flora. Ten - fold dilutions of C. fetus subs. venerealis NCTC 10354 in PBS or preputial washing with or without centrifugation were used. All experiments were done in duplicate by three observers. Positive and negative controls were included in each assay. The detection li- mits of DFAT were 104 CFU/ml for PBS and non - centrifuged preputial washings and 102 CFU/ml for centrifuged preputial washing. There was no observer effect. The sensitivity and specificity of DFAT were 92.59% and 88.89%, respectively. The DFAT was observed to be sensitive, specific and the effect of experienced observers was minimal on test per- formance.
650 $aCampylobacter Fetus
650 $aDiagnostico
653 $aAntibody test
653 $aBovine genital campylobacteriosis
653 $aCampilobacteriosis genital bobina
653 $aDiagnosis
653 $aDirect fluorescent
653 $aImunofluorescencia direta
653 $aLavado prepucial
653 $aPreputial washing
700 1 $aPELLEGRIN, A. O.
700 1 $aFOSCOLO, C. B.
700 1 $aMACHADO, R. P.
700 1 $aMIRANDA, K. L.
700 1 $aLAGE, A. P.
773 $tRevista Latinoamericana de Microbiologia$gv.44, n.3/4, p.118-123, 2002.
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Registro original: |
Embrapa Pantanal (CPAP) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
05/03/2021 |
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Data da última atualização: |
11/11/2022 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
ZAMAN, M.; KLEINEIDAM, K.; BAKKEN, L.; BERENDT, J.; BRACKEN, C.; BUTTERBACH-BAHL, K.; CAI, Z.; CHANG, S. X.; CLOUGH, T.; DAWAR, K.; DING, W. X.; DÖRSCH, P.; MARTINS, M. dos R.; ECKHARDT, C.; FIEDLER, T.; FROSCH, T.; GOOPY, J.; GORRES, C. M.; GUPTA, A.; HENJES, S.; HOFMMAN, M. E. G.; HORN, M. A.; JAHANGIR, M. M. R.; JANSEN-WILLEMS, A.; LENHART, K.; HENG, L.; LEWICKA-SZCZEBAK, D.; LUCIC, G.; MERBOLD, L.; MOHN, J.; MOLSTAD, L.; MOSER, G.; MURPHY, P.; SANZ-COBENA, A.; SIMEK, M.; URQUIAGA, S.; WELL, R.; WRAGE-MÖNNIG, N.; ZAMAN, S.; SHANG, J.; MÜLLER, C. |
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Título: |
Automated laboratory and field techniques to determine greenhouse gas emissions. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: ZAMAN, M.; HENG, L.; Müller, C. (Ed.). Measuring emission of agricultural greenhouse gases and developing mitigation options using nuclear and related techniques: applications of nuclear techniques for GHGs. London: Springer, 2021. Chapter 3. |
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Páginas: |
p. 109-139 |
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ISBN: |
978-3-030-55396-8 |
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DOI: |
https://doi.org/10.1007/978-3-030-55396-8_ |
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Idioma: |
Inglês |
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Conteúdo: |
Methods and techniques are described for automated measurements of greenhouse gases (GHGs) in both the laboratory and the field. Robotic systems are currently available to measure the entire range of gases evolved from soils including dinitrogen (N2). These systems usually work on an exchange of the atmospheric N2 with helium (He) so that N2 fluxes can be determined. Laboratory systems are often used in microbiology to determine kinetic response reactions via the dynamics of all gaseous N species such as nitric oxide (NO), nitrous oxide (N2O), and N2. Latest He incubation techniques also take plants into account, in order to study the effect. of plant?soil interactions on GHGs and N2 production. |
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Thesaurus NAL: |
cattle; climate change; greenhouse gas emissions; urine. |
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Categoria do assunto: |
-- |
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Marc: |
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008 2021 bl uuuu u00u1 u #d
020 $a978-3-030-55396-8
024 7 $ahttps://doi.org/10.1007/978-3-030-55396-8_$2DOI
100 1 $aZAMAN, M.
245 $aAutomated laboratory and field techniques to determine greenhouse gas emissions.$h[electronic resource]
260 $c2021
300 $ap. 109-139
520 $aMethods and techniques are described for automated measurements of greenhouse gases (GHGs) in both the laboratory and the field. Robotic systems are currently available to measure the entire range of gases evolved from soils including dinitrogen (N2). These systems usually work on an exchange of the atmospheric N2 with helium (He) so that N2 fluxes can be determined. Laboratory systems are often used in microbiology to determine kinetic response reactions via the dynamics of all gaseous N species such as nitric oxide (NO), nitrous oxide (N2O), and N2. Latest He incubation techniques also take plants into account, in order to study the effect. of plant?soil interactions on GHGs and N2 production.
650 $acattle
650 $aclimate change
650 $agreenhouse gas emissions
650 $aurine
700 1 $aKLEINEIDAM, K.
700 1 $aBAKKEN, L.
700 1 $aBERENDT, J.
700 1 $aBRACKEN, C.
700 1 $aBUTTERBACH-BAHL, K.
700 1 $aCAI, Z.
700 1 $aCHANG, S. X.
700 1 $aCLOUGH, T.
700 1 $aDAWAR, K.
700 1 $aDING, W. X.
700 1 $aDÖRSCH, P.
700 1 $aMARTINS, M. dos R.
700 1 $aECKHARDT, C.
700 1 $aFIEDLER, T.
700 1 $aFROSCH, T.
700 1 $aGOOPY, J.
700 1 $aGORRES, C. M.
700 1 $aGUPTA, A.
700 1 $aHENJES, S.
700 1 $aHOFMMAN, M. E. G.
700 1 $aHORN, M. A.
700 1 $aJAHANGIR, M. M. R.
700 1 $aJANSEN-WILLEMS, A.
700 1 $aLENHART, K.
700 1 $aHENG, L.
700 1 $aLEWICKA-SZCZEBAK, D.
700 1 $aLUCIC, G.
700 1 $aMERBOLD, L.
700 1 $aMOHN, J.
700 1 $aMOLSTAD, L.
700 1 $aMOSER, G.
700 1 $aMURPHY, P.
700 1 $aSANZ-COBENA, A.
700 1 $aSIMEK, M.
700 1 $aURQUIAGA, S.
700 1 $aWELL, R.
700 1 $aWRAGE-MÖNNIG, N.
700 1 $aZAMAN, S.
700 1 $aSHANG, J.
700 1 $aMÜLLER, C.
773 $tIn: ZAMAN, M.; HENG, L.; Müller, C. (Ed.). Measuring emission of agricultural greenhouse gases and developing mitigation options using nuclear and related techniques: applications of nuclear techniques for GHGs. London: Springer, 2021. Chapter 3.
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