| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
10/08/2018 |
|
Data da última atualização: |
24/09/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
NICIURA, S. C. M.; CRUVINEL, G. G.; MORAES, C. V.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; CHAGAS, A. C. de S. |
|
Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; Giovanna Gabrielle Cruvinel, UNICEP; Caroline Valério Moraes, UFSCar; FLAVIA ALINE BRESSANI DONATONI, CPPSE; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
|
Título: |
PCR-based genotyping of SNP markers in sheep. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Molecular Biology Reports, v.45, n.4, p.651-654, aug. 2018. |
|
ISSN: |
1573-4978 |
|
DOI: |
https://doi.org/10.1007/s11033-018-4206-8 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Single nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. |
|
Palavras-Chave: |
PCR RFLP; Resistência nematódeo gastrintestinal; Tetra primer ARMS PCR. |
|
Thesagro: |
Marcador Molecular; Ovino. |
|
Thesaurus Nal: |
Genome. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02300naa a2200289 a 4500
001 2094070
005 2018-09-24
008 2018 bl uuuu u00u1 u #d
022 $a1573-4978
024 7 $ahttps://doi.org/10.1007/s11033-018-4206-8$2DOI
100 1 $aNICIURA, S. C. M.
245 $aPCR-based genotyping of SNP markers in sheep.$h[electronic resource]
260 $c2018
520 $aSingle nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays.
650 $aGenome
650 $aMarcador Molecular
650 $aOvino
653 $aPCR RFLP
653 $aResistência nematódeo gastrintestinal
653 $aTetra primer ARMS PCR
700 1 $aCRUVINEL, G. G.
700 1 $aMORAES, C. V.
700 1 $aDONATONI, F. A. B.
700 1 $aMALAGO JUNIOR, W.
700 1 $aBENAVIDES, M. V.
700 1 $aCHAGAS, A. C. de S.
773 $tMolecular Biology Reports$gv.45, n.4, p.651-654, aug. 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Amazônia Oriental. |
|
Data corrente: |
11/05/1998 |
|
Data da última atualização: |
26/01/2015 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
RODRIGUES, I. A.; SERRA, A. G. P. |
|
Afiliação: |
IRENICE ALVES RODRIGUES, CPATU. |
|
Título: |
Coleta, propagação e avaliação de plantas medicinais da Amazônia. |
|
Ano de publicação: |
1997 |
|
Fonte/Imprenta: |
In: SIMPÓSIO LATINO-AMERICANO DE RECURSOS GENÉTICOS VEGETAIS, 1., 1997, Campinas. Programas e resumos. Campinas: IAC; Brasília, DF: EMBRAPA-CENARGEN, 1997. |
|
Páginas: |
p. 95-96. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Adaptation; Brasil; Drug plants; Ipeca; Policarpus microphyllus; Propagação. |
|
Thesagro: |
Aclimatação; Jaborandi; Planta Medicinal; Psychotria Ipecacuanha. |
|
Thesaurus NAL: |
Amazonia; plant propagation. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/116439/1/p9596.pdf
|
|
Marc: |
LEADER 00830nam a2200265 a 4500
001 1394543
005 2015-01-26
008 1997 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, I. A.
245 $aColeta, propagação e avaliação de plantas medicinais da Amazônia.
260 $aIn: SIMPÓSIO LATINO-AMERICANO DE RECURSOS GENÉTICOS VEGETAIS, 1., 1997, Campinas. Programas e resumos. Campinas: IAC; Brasília, DF: EMBRAPA-CENARGEN$c1997
300 $ap. 95-96.
650 $aAmazonia
650 $aplant propagation
650 $aAclimatação
650 $aJaborandi
650 $aPlanta Medicinal
650 $aPsychotria Ipecacuanha
653 $aAdaptation
653 $aBrasil
653 $aDrug plants
653 $aIpeca
653 $aPolicarpus microphyllus
653 $aPropagação
700 1 $aSERRA, A. G. P.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
