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| Registros recuperados : 101 | |
| 4. |  | ORTIZ, W. H.; QUIRINO, C. R.; SILVA, A.; OLIVEIRA, C. S.; SERAPIÃO, R. V.; PACHECO, A.; BARTHOLAZZI, A. Association between BMP4 gene polymorphism and in vitro embryo production traits in Gyr cows. Revista Colombiana de Ciencias Pecuarias, v. 28, n. 2, p. 156-164, 2015.| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; LIMA, M. R. DE; GARCIA, J. M. Global H3K9AC and H3K27ME3 expression in blastomeres from 8-to 16-cell stage bovine embryos. Reproduction, Fertility and Development , v. 26, n. 1, p. 148-149, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno.| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | LEAL, G. R.; GRACIOSA, M. A. G.; PASOLINI, R.; MONTEIRO, C. A. S.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Adaptação do sistema SPOM melhora a taxa de produção in vitro de embriões bovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | DIAS, N. B.; CAMARGO, L. S. A.; SERAPIÃO, R. V.; FOLHADELLA, I. M.; RAMOS, A. A.; POLISSENI, J.; VIANA, J. H. M.; SÁ, W. F.; FERREIRA, A. M. Association of bovine sperm motility and viability with in vitro embryo production. Acta Scientiae Veterinariae, v. 34, s437, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | BOITÉ, M. C.; CAMARGO, L. S. A.; GUIMARÃES, M. F. M.; SERAPIÃO, R. V.; WOHLRES-VIANA, S.; VIANA, J. H. M.; SÁ, W. F.; NOGUEIRA, L. A. G. Analysis of gene expression of in vitro produced bovine blastocyst cultured for 72 hours post-vitrification. In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 16., 2008, Budapest. Abstracts... Budapest, 2008. p. 186| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | OLIVEIRA, C. S.; SERAPIÃO, R. V.; SARAIVA, N. Z.; CAMARGO, A. J. dos R.; FERREIRA, M. B. D.; FREITAS, C. de; GARCIA, J. M.; VERNEQUE, R. da S. Análise quantitativa de lipídeos em oócitos de vacas leiteiras das raças Gir Leiteiro, Holandesa, e mestiças F1. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | PEREIRA, M. M.; SERAPIÃO, R. V.; QUINTÃO, C. R. C.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Apoptose em embriões bovinos produzidos in vitro utilizando oócitos maturados em meio livre de soro e baixa tensão de oxigênio. Acta Scientiae Veterinariae, v. 38, p. 383, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | PEREIRA, M. M.; SERAPIAO, R. V.; QUINTAO, C. C. R.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Apoptosis in bovine embryos produced with oocytes matured in serum-free medium under low oxygen tension. Acta Scientiae Veterinariae, v. 38, p. s675, 2010. Supl.2.
Edição dos resumos da 25a. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, PE, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | CAMARGO, L. S. A.; VIANA, J. H. M.; SERAPIÃO, R. V.; GUIMARÃES, M. F. M.; SÁ, W. F. de; FERREIRA, A. de M. Assessment of HSP70-1 transcription levels in immature oocytes from Bos Taurus and Bos Indicus cows raised in a tropical climate. Reproduction, Fertility and Development, v. 19, n. 1, p. 247-248, 2007. Edição dos proceedings do Annual Conference of The Internatinal Embryo Transfer Society, 2007, Kyoto.| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | ROSA, P. M. S.; MONTEIRO, C. A. S.; LEAL, G. R.; CAMARGO, A. J. R.; SERAPIÃO, R. V.; LIMA, M. R.; GARCIA, J. M.; OLIVEIRA, C. S. Association of two epigenetic modulators on the initial development of female bovine embryos. Animal Reproduction, v. 15, n. 3, p. 484, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | VEGA, W. H. O.; QUIRINO, C. R.; AZEVEDO, A. S. de; PACHECO, A.; OLIVEIRA, C. S.; SERAPIÃO, R. V.; BARTHOLAZZI, A. J. Associação entre polimorfismo no gene BMP4 e características de OPU-PIVE em vacas Gir. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 24., 2014, Vitória. Anais... Vitória: Universidade Federal do Espírito Santo, 2014. 3 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASSOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Caracterização de oócitos de animais pré-púberes da raga Gir: tamnho do oócito, morfologia e atividade metabólica. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | VEGA, W. H. O.; QUIRINO, C. R.; JUNIOR, A. B.; DAVID, C. M. G.; JANDIM, J. G.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Diversidade genética em populações de gado de elite associadas a biotecnologias reprodutivas. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 12., 2017, Ribeirão Preto. Anais... Ribeirão Preto: SBMA, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Hormonal ovulation induction in prepubertal GYR heifers In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tours: Society for Reproductive Biology, 2016. p. 516-517.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 101 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CAMARGO, L. S. de A.; BOITE, M. C.; WOHLRES-VIANA, S.; MOTA, G. B.; SERAPIÃO, R. V.; SÁ, W. F. de; VIANA, J. H. M.; NOGUEIRA, L. A. G. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARIANA CORTES BOITE, FIOCRUZ; SABINE WOHLRES-VIANA, UFJF; GUSTAVO BRUNO MOTA, UNB; RAQUEL VARELA SERAPIÃO, PESAGRO; WANDERLEI FERREIRA DE SÁ, Pesquisador aposentado do CNPGL; JOAO HENRIQUE MOREIRA VIANA, CNPGL; LUIZ ALTAMIRO GARCIA NOGUEIRA, UFF. |
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Título: |
Osmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Cryobiology, v. 63, n. 3, p. 256-262, 2011. |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2011.09.135 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system. MenosThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-... Mostrar Tudo |
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Palavras-Chave: |
Embryo. |
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Thesaurus NAL: |
cryopreservation; osmosis; vitrification. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02924naa a2200265 a 4500
001 1910654
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2011.09.135$2DOI
100 1 $aCAMARGO, L. S. de A.
245 $aOsmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro.$h[electronic resource]
260 $c2011
520 $aThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system.
650 $acryopreservation
650 $aosmosis
650 $avitrification
653 $aEmbryo
700 1 $aBOITE, M. C.
700 1 $aWOHLRES-VIANA, S.
700 1 $aMOTA, G. B.
700 1 $aSERAPIÃO, R. V.
700 1 $aSÁ, W. F. de
700 1 $aVIANA, J. H. M.
700 1 $aNOGUEIRA, L. A. G.
773 $tCryobiology$gv. 63, n. 3, p. 256-262, 2011.
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