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Registros recuperados : 101 | |
4. | | ORTIZ, W. H.; QUIRINO, C. R.; SILVA, A.; OLIVEIRA, C. S.; SERAPIÃO, R. V.; PACHECO, A.; BARTHOLAZZI, A. Association between BMP4 gene polymorphism and in vitro embryo production traits in Gyr cows. Revista Colombiana de Ciencias Pecuarias, v. 28, n. 2, p. 156-164, 2015. Biblioteca(s): Embrapa Gado de Leite. |
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5. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; LIMA, M. R. DE; GARCIA, J. M. Global H3K9AC and H3K27ME3 expression in blastomeres from 8-to 16-cell stage bovine embryos. Reproduction, Fertility and Development , v. 26, n. 1, p. 148-149, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno. Biblioteca(s): Embrapa Gado de Leite. |
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9. | | LEAL, G. R.; GRACIOSA, M. A. G.; PASOLINI, R.; MONTEIRO, C. A. S.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Adaptação do sistema SPOM melhora a taxa de produção in vitro de embriões bovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. Biblioteca(s): Embrapa Gado de Leite. |
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10. | | DIAS, N. B.; CAMARGO, L. S. A.; SERAPIÃO, R. V.; FOLHADELLA, I. M.; RAMOS, A. A.; POLISSENI, J.; VIANA, J. H. M.; SÁ, W. F.; FERREIRA, A. M. Association of bovine sperm motility and viability with in vitro embryo production. Acta Scientiae Veterinariae, v. 34, s437, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006. Biblioteca(s): Embrapa Gado de Leite. |
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11. | | BOITÉ, M. C.; CAMARGO, L. S. A.; GUIMARÃES, M. F. M.; SERAPIÃO, R. V.; WOHLRES-VIANA, S.; VIANA, J. H. M.; SÁ, W. F.; NOGUEIRA, L. A. G. Analysis of gene expression of in vitro produced bovine blastocyst cultured for 72 hours post-vitrification. In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 16., 2008, Budapest. Abstracts... Budapest, 2008. p. 186 Biblioteca(s): Embrapa Gado de Leite. |
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12. | | OLIVEIRA, C. S.; SERAPIÃO, R. V.; SARAIVA, N. Z.; CAMARGO, A. J. dos R.; FERREIRA, M. B. D.; FREITAS, C. de; GARCIA, J. M.; VERNEQUE, R. da S. Análise quantitativa de lipídeos em oócitos de vacas leiteiras das raças Gir Leiteiro, Holandesa, e mestiças F1. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM. Biblioteca(s): Embrapa Gado de Leite. |
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13. | | PEREIRA, M. M.; SERAPIÃO, R. V.; QUINTÃO, C. R. C.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Apoptose em embriões bovinos produzidos in vitro utilizando oócitos maturados em meio livre de soro e baixa tensão de oxigênio. Acta Scientiae Veterinariae, v. 38, p. 383, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010. Biblioteca(s): Embrapa Gado de Leite. |
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14. | | PEREIRA, M. M.; SERAPIAO, R. V.; QUINTAO, C. C. R.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Apoptosis in bovine embryos produced with oocytes matured in serum-free medium under low oxygen tension. Acta Scientiae Veterinariae, v. 38, p. s675, 2010. Supl.2.
Edição dos resumos da 25a. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, PE, 2010. Biblioteca(s): Embrapa Gado de Leite. |
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15. | | CAMARGO, L. S. A.; VIANA, J. H. M.; SERAPIÃO, R. V.; GUIMARÃES, M. F. M.; SÁ, W. F. de; FERREIRA, A. de M. Assessment of HSP70-1 transcription levels in immature oocytes from Bos Taurus and Bos Indicus cows raised in a tropical climate. Reproduction, Fertility and Development, v. 19, n. 1, p. 247-248, 2007. Edição dos proceedings do Annual Conference of The Internatinal Embryo Transfer Society, 2007, Kyoto. Biblioteca(s): Embrapa Gado de Leite. |
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16. | | ROSA, P. M. S.; MONTEIRO, C. A. S.; LEAL, G. R.; CAMARGO, A. J. R.; SERAPIÃO, R. V.; LIMA, M. R.; GARCIA, J. M.; OLIVEIRA, C. S. Association of two epigenetic modulators on the initial development of female bovine embryos. Animal Reproduction, v. 15, n. 3, p. 484, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018. Biblioteca(s): Embrapa Gado de Leite. |
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17. | | VEGA, W. H. O.; QUIRINO, C. R.; AZEVEDO, A. S. de; PACHECO, A.; OLIVEIRA, C. S.; SERAPIÃO, R. V.; BARTHOLAZZI, A. J. Associação entre polimorfismo no gene BMP4 e características de OPU-PIVE em vacas Gir. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 24., 2014, Vitória. Anais... Vitória: Universidade Federal do Espírito Santo, 2014. 3 p. Biblioteca(s): Embrapa Gado de Leite. |
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18. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASSOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Caracterização de oócitos de animais pré-púberes da raga Gir: tamnho do oócito, morfologia e atividade metabólica. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. Biblioteca(s): Embrapa Gado de Leite. |
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19. | | VEGA, W. H. O.; QUIRINO, C. R.; JUNIOR, A. B.; DAVID, C. M. G.; JANDIM, J. G.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Diversidade genética em populações de gado de elite associadas a biotecnologias reprodutivas. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 12., 2017, Ribeirão Preto. Anais... Ribeirão Preto: SBMA, 2017. Biblioteca(s): Embrapa Gado de Leite. |
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20. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Hormonal ovulation induction in prepubertal GYR heifers In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tours: Society for Reproductive Biology, 2016. p. 516-517. Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 101 | |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
17/10/2007 |
Data da última atualização: |
17/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
CAMARGO, L. S. de A.; VIANA, J. H. M.; RAMOS, A. A.; SERAPIÃO, R. V.; SÁ, W. F.; FERREIRA, A. M.; GUIMARÃES, M. F. M.; VALE FILHO, V. R. do. |
Afiliação: |
Luiz Sérgio Almeida Camargo, Embrapa Gado de Leite; João Henrique Moreira Viana, Embrapa Gado de Leite; Alessandra A. Ramos, Fiocruz; Raquel Varela Serapião, UENF; Wanderlei Ferreira de Sá, Embrapa Gado de Leite; Ademir de Moraes Ferreira, Embrapa Gado de Leite; Marta Fonseca Martins Guimaraes, Embrapa Gado de Leite; V. R. do Vale Filho, UFMG. |
Título: |
Developmental competence and expression of the Hsp 70.1 gene in oocytes obtained from Bos indicus and Bos taurus dairy cows in a tropical environment. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Theriogenology, New York, v. 68, n. 4, p. 626-632, 2007. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2007.03.029 |
Idioma: |
Inglês |
Conteúdo: |
Bos indicus cows usually have better reproductive performance in tropical and subtropical regions than Bos taurus cows, presumably due to their better adaptation to tropical environments. The aim of this study was to evaluate the developmental competence and expression of the Hsp 70.1 gene in immature oocytes from B. taurus (Holstein) and B. indicus (Gyr) dairy cows raised in a tropical region. Cumulus–oocyte complexes were obtained by transvaginal ultrasound-guided follicle aspiration between spring and early autumn, and subjected to in vitro maturation and fertilization. Presumptive zygotes were co-cultured with their own cumulus cells in CR2aa media with 10% fetal calf serum; Grade 1 blastocysts were transferred to synchronized crossbred recipients. The total RNA was extracted from immature Holstein and Gyr oocytes (three pools for each breed) and relative quantification of the Hsp 70.1 transcripts was performed by real time PCR after reverse transcription. Cleavage and blastocyst rates were greater (P < 0.05) for Gyr (n = 390 oocytes) than Holstein (n = 505) breed (66.7% versus 53.1% of cleavage and 19.6% versus 10.8% of blastocysts, respectively), but pregnancy rates were not significantly different following transfer to recipients (44.5% for 36 Gyr embryos; 60% for 10 Holstein embryos). Holstein immature oocytes had a higher level (P < 0.05) of Hsp 70.1 relative expression (1.82 ± 0.22; mean ± S.E.M.) than Gyr oocytes (1.12 ± 0.11). In conclusion, Gyr oocytes obtained in a tropical region were less subject to stress and more likely to develop (after IVF) than Holstein oocytes. MenosBos indicus cows usually have better reproductive performance in tropical and subtropical regions than Bos taurus cows, presumably due to their better adaptation to tropical environments. The aim of this study was to evaluate the developmental competence and expression of the Hsp 70.1 gene in immature oocytes from B. taurus (Holstein) and B. indicus (Gyr) dairy cows raised in a tropical region. Cumulus–oocyte complexes were obtained by transvaginal ultrasound-guided follicle aspiration between spring and early autumn, and subjected to in vitro maturation and fertilization. Presumptive zygotes were co-cultured with their own cumulus cells in CR2aa media with 10% fetal calf serum; Grade 1 blastocysts were transferred to synchronized crossbred recipients. The total RNA was extracted from immature Holstein and Gyr oocytes (three pools for each breed) and relative quantification of the Hsp 70.1 transcripts was performed by real time PCR after reverse transcription. Cleavage and blastocyst rates were greater (P < 0.05) for Gyr (n = 390 oocytes) than Holstein (n = 505) breed (66.7% versus 53.1% of cleavage and 19.6% versus 10.8% of blastocysts, respectively), but pregnancy rates were not significantly different following transfer to recipients (44.5% for 36 Gyr embryos; 60% for 10 Holstein embryos). Holstein immature oocytes had a higher level (P < 0.05) of Hsp 70.1 relative expression (1.82 ± 0.22; mean ± S.E.M.) than Gyr oocytes (1.12 ± 0.11). In conclusion, Gyr oocytes obtained ... Mostrar Tudo |
Palavras-Chave: |
heat shock protein; oocyte. |
Thesagro: |
Bos Indicus. |
Thesaurus NAL: |
heat stress; in vitro fertilization. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/596073/1/Developmental-competence-and-expression-of-the-Hsp-70.1-gene-in-oocytes.pdf
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Marc: |
LEADER 02532naa a2200277 a 4500 001 1596073 005 2024-02-17 008 2007 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2007.03.029$2DOI 100 1 $aCAMARGO, L. S. de A. 245 $aDevelopmental competence and expression of the Hsp 70.1 gene in oocytes obtained from Bos indicus and Bos taurus dairy cows in a tropical environment.$h[electronic resource] 260 $c2007 520 $aBos indicus cows usually have better reproductive performance in tropical and subtropical regions than Bos taurus cows, presumably due to their better adaptation to tropical environments. The aim of this study was to evaluate the developmental competence and expression of the Hsp 70.1 gene in immature oocytes from B. taurus (Holstein) and B. indicus (Gyr) dairy cows raised in a tropical region. Cumulus–oocyte complexes were obtained by transvaginal ultrasound-guided follicle aspiration between spring and early autumn, and subjected to in vitro maturation and fertilization. Presumptive zygotes were co-cultured with their own cumulus cells in CR2aa media with 10% fetal calf serum; Grade 1 blastocysts were transferred to synchronized crossbred recipients. The total RNA was extracted from immature Holstein and Gyr oocytes (three pools for each breed) and relative quantification of the Hsp 70.1 transcripts was performed by real time PCR after reverse transcription. Cleavage and blastocyst rates were greater (P < 0.05) for Gyr (n = 390 oocytes) than Holstein (n = 505) breed (66.7% versus 53.1% of cleavage and 19.6% versus 10.8% of blastocysts, respectively), but pregnancy rates were not significantly different following transfer to recipients (44.5% for 36 Gyr embryos; 60% for 10 Holstein embryos). Holstein immature oocytes had a higher level (P < 0.05) of Hsp 70.1 relative expression (1.82 ± 0.22; mean ± S.E.M.) than Gyr oocytes (1.12 ± 0.11). In conclusion, Gyr oocytes obtained in a tropical region were less subject to stress and more likely to develop (after IVF) than Holstein oocytes. 650 $aheat stress 650 $ain vitro fertilization 650 $aBos Indicus 653 $aheat shock protein 653 $aoocyte 700 1 $aVIANA, J. H. M. 700 1 $aRAMOS, A. A. 700 1 $aSERAPIÃO, R. V. 700 1 $aSÁ, W. F. 700 1 $aFERREIRA, A. M. 700 1 $aGUIMARÃES, M. F. M. 700 1 $aVALE FILHO, V. R. do 773 $tTheriogenology, New York$gv. 68, n. 4, p. 626-632, 2007.
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