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| Registros recuperados : 112 | |
| 81. |  | NASCIMENTO, W. B. A.; SANTOS, C. M. dos; CESAR, M. J. da S. C.; BALDANI, J. I.; VIDAL, M. S.; SCHWAB, S. Avaliação do potencial de novos isolados de bactérias do gênero Sphingomonas para uso como inoculante na cultura da cana-de-açúcar. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER 21., 2021, Seropédica. A transversalidade da ciência, tecnologia e inovações para o planeta: caderno de resumos. Seropédica: Embrapa Agrobiologia, 2021.| Biblioteca(s): Embrapa Agrobiologia. |
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| 82. | | BALDANI, J. I.; CRUZ, L. M.; VIDAL, M. S.; ARAUJO, J. L. S. de; SCHWAB, S.; TEIXEIRA, K. R. dos S.; SOUZA, E. M.; PEDROSA, F. O.; FARINELLI. Draft genome sequente of six endophytic nittrogen-fixing bacteria with potential biofertilizer application in non-leguminous plants. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. Slll. 05| Biblioteca(s): Embrapa Agrobiologia. |
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| 83. | | GUEDES, H. V.; RUWS, L F. M.; ALQUÉRES, S. M. C.; SCHWAB, S.; ARAUJO, J. L. S. de; MARTINS, O. B.; BALDANI, J. I. Análise de expressão da nitrogenase, glutamato sintase por em um mutante histidina quinase (GDI3079) de gluconacetobacter diazotrophicus In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25.,2009, Porto de Galinhas, PE. Anais... [São Paulo]: Sociedade Bras. de Microbiologia, 2009. 01 01 de 01| Biblioteca(s): Embrapa Agrobiologia. |
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| 84. | | BALDANI, J. I.; VIDEIRA, S. S.; QUESSADA, D.; BALDANI, V. L. D.; VIDAL, M. S.; SCHWAB, S.; ARAUJO, J. L. S. de; URQUIAGA, S. BNF in the bioenergy Pennisetum purpureum In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 15.; SYMPOSIUM OF THE EUROPEAN NITROGEN FIXATION CONFERENCE, 12., 2016, Hungary. Book of abstracts. Hungary: Biological Research Center, 2016.| Biblioteca(s): Embrapa Agrobiologia. |
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| 85. | | SCHWAB, S.; PESSOA, C. A.; BERGAMI, A. A. de L.; FIGUEIREDO, N. L. A.; TEIXEIRA, K. R. dos S.; BALDANI, J. I. Isolation and characterization of active promoters from Gluconacetobacter diazotrophicus strain PAL5 using a promoter-trapping plasmid Archives of Microbiology, v. 198, n. 5, p. 445?458, 2016.| Biblioteca(s): Embrapa Agrobiologia. |
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| 86. | | FERREIRA, N. S.; MATOS, G. F.; MENESES, C. H. G. de; REIS, V. M.; ROUWS, J. R. C.; SCHWAB, S.; BALDANI, J. I.; ROUWS, L. F. M. Interaction of phytohormone-producing rhizobia with sugarcane mini-setts and their effect on plant development Plant and Soil, Published online 3 January, 2020.| Biblioteca(s): Embrapa Agrobiologia. |
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| 87. | | FERREIRA, V. de L.; GALISA, P. de S.; SCHWAB, S.; VIDAL, M. S.; BALDANI, J. I.; ARAUJO, J. L. S. de. Caracterização da função de MatE em Gluconacetobacter diazotrophicus. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010.| Biblioteca(s): Embrapa Agrobiologia. |
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| 88. | | SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. Designing and validation of specifc primers for the quantitative detection of bacteria in sugarcane inoculant. Brazilian Journal of Microbiology, v. 54, p. 2627–2640, 2023.| Biblioteca(s): Embrapa Agrobiologia. |
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| 89. | | SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant. Brazilian Journal of Microbiology,, Published: 16 October 2023| Biblioteca(s): Embrapa Agrobiologia. |
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| 90. | | SANTOS, C. M dos; SOUZA, D. G. de; BALSANELLI, E; CRUZ, L. M.; SOUZA, E. M. de; BALDANI, J. I.; SCHWAB, S. A culture independent approach to enrich endophytic bacterial cells from sugarcane stems for community characterization. Microbiol Ecology, v. 74, n. 2, p. 453-465, 2017.| Biblioteca(s): Embrapa Agrobiologia. |
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| 91. | | CAMELO, A.; BARRETO, C. P.; VIDAL, M. S.; ROUWS, J. R. C.; LEDO, F. J. da S.; SCHWAB, S.; BALDANI, J. I. Field response of two seed propagated elephant grass genotypes to diazotrophic bacterial inoculation and in situ confocal microscopy colonization analyses. Symbiosis, v. 83, n. 1, p. 41-53, 2021| Biblioteca(s): Embrapa Agrobiologia; Embrapa Gado de Leite. |
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| 92. | | TERRA, L. A.; SOARES, C. de P.; TADRA-SFEIR, M. Z.; SOUZA, E. M.; SILVEIRA, V.; BALDANI, J. I.; VIDAL, M. S.; SCHWAB, S. Transcriptome and proteome approaches for the diazotroph Nitrospirillum amazonense grown in sugarcane apoplast fluid. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 52 Session 4, Poster IV.5| Biblioteca(s): Embrapa Agrobiologia. |
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| 93. | | TERRA, L. A.; SOARES, C. de P.; MENESES, C. H. G. de; SFEIR, T.; SOUZA, E. M. de; SILVEIRA, V.; VIDAL, M. S.; BALDANI, J. I.; SCHWAB, S. Transcriptome and proteome profiles of the diazotroph Nitrospirillum amazonense strain CBAmC in response to the sugarcane apoplast fluid Plant and Soil, v. 451, p. 145-168, 2020.| Biblioteca(s): Embrapa Agrobiologia. |
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| 94. | | SANTOS, C. M. dos; BALSANELLI, E.; SOUZA, E. M.; RIBERIRO, N. V. S.; SCHWAB, S.; VIDAL, M. S.; BALDANI, J. I. Understanding the apoplast bacterial community from sugarcane inoculated with a consortium of fi ve diazotrophic bacteria. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16.; LATINAMERICAN WORKSHOP OF PGPR, 4.; RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Embrapa, 2018. p. 79 Session 6, Poster VI.33| Biblioteca(s): Embrapa Agrobiologia. |
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| 95. | | SCHWAB, S.; CRIVELARO, A. L. R.; WEISS, V. A.; SFEIR, M. Z. T.; FAORO, H.; CRUZ, L. M.; PEDROZA, F. de O.; SOUZA, E. M. de; BALDANI, J. I. Global transcriptional shift analysis of nitrogenase switch-off by glutamate in azospirillum amazonense strain CBAMC, isolated from sugarcane. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 13., 2012, Neuherberg, GE. Abstracts... Neuherberg: German Research Center for Environmental Health, 2012.| Biblioteca(s): Embrapa Agrobiologia. |
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| 96. | | ROUWS, L. F. M.; MENESES, C. H. G.; GALVÃO, P. G.; VENTURA, H, G.; DRECHSEL, M. M.; SILVA, R. J.; VIDAL, M. S.; SIMÕES-ARAUJO, J. L.; TEIXEIRA, K. R. S.; SCHWAB, S.; BALDANI, J. I. Functional analysis of Gluconacetobacter diazotrophicus PAL5 genes involved in the nitogen fixation process and interaction with the plant. INTERNATIONAL INCT SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 1., 2009, Curitiba. Program and index... Brasilia: INCT, 2009. 01p| Biblioteca(s): Embrapa Agrobiologia. |
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| 97. | | ROUWS, L. F. M.; MENESSES, C. H. G.; GALVÃO, P. G.; VENTURA, H. G.; DRECHSEL, M. M.; SILVA, R. J.; VIDAL, M. S.; SIMÕES-ARAUJO, J. L.; TEIXEIRA, K. R. S.; SCHWAB, S.; BALDANI, J. I. Functional analysis of Gluconacetobacter diazotrophicus PAL5 genes involved in the nitrogen fixation processs and interaction with the plant. INTERNATIONAL INCT SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 1., 2009, Curitiba. Program and index... Brasilia: INCT, 2009. 01p| Biblioteca(s): Embrapa Agrobiologia. |
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| 98. | | REIS, V. M.; PITARD, R. M.; DOURADO, F. dos S.; CAMACHO, N. N.; VIDEIRA, S. S.; CAMARGO, A. B.; BOA SORTE, P. F.; VIDAL, M. S.; SCHWAB, S.; BALDANI, J. I. Caracterização morfo-fisiológica e molecular das estirpres diazotróficas do inoculante de cana-de-açúcar. Seropédica: Embrapa Agrobiologia, 2011. (Embrapa Agrobiologia. Documentos, 280).| Biblioteca(s): Embrapa Agrobiologia. |
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| 99. | | BALDANI, J. I.; VIDEIRA, S. S.; TEIXEIRA, K. R. dos S.; REIS, V. M.; OLIVERIA, A. L. M. de; SCHWAB, S.; SOUZA, E. M.; PEDRAZA, R. O.; BALDANI, V. L. D.; HARTMANN, A. The family Rhodospirillaceae. In: ROSENBERG, E. (Ed.). The prokaryotes: Alphaproteobacteria and Betaproteobacteria. 4. ed. Heidelberg: Springer, 2014. p. 533-618| Biblioteca(s): Embrapa Agrobiologia. |
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| 100. | | SCHWAB, S.; MENEZES, J. dos S.; NASCIMENTO, W. B. A.; NASCIMENTO, B. P. do; FERREIRA, J. de P.; SANTOS, C. M. dos; VIGNA, B. B. Z.; MEIRELES, K. G. X.; VIDAL, M. S.; BALDANI, J. I. Desinfestação de sementes de plantas dos gêneros Paspalum e Brachiaria para ensaios de inoculação in vitro de bactéria fixadora de nitrogênio. Seropédica: Embrapa Agrobiologia, 2020. 22 p. (Embrapa Agrobiologia. Documentos, 314).| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 112 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
07/03/2023 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SCHWAB, S.; HIRATA, E. S.; AMARAL, J. C. A.; SILVA, C. G. N.; FERREIRA, J. P.; SILVA, L. V. da; ROUWS, J. R. C.; ROUWS, L. F. M.; BALDANI, J. I.; REIS, V. M. |
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Afiliação: |
STEFAN SCHWAB, CNPAB; BASF S.A; J. C. A. AMARAL, UFRRJ; C. G. N. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; J. P. FERREIRA, SOLUBIO; L. V. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; JANAINA RIBEIRO COSTA ROUWS, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB; JOSE IVO BALDANI, CNPAB; VERONICA MASSENA REIS, CNPAB. |
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Título: |
Quantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods, |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant and Soil , Published: 22 February 2023. |
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ISSN: |
1573-5036 |
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Idioma: |
Inglês |
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Conteúdo: |
Nitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion of the mini-setts in bacterial suspension proved to be more efficient than the inoculation methods of applying the suspension onto the post-sprouting substrate or to the lowest lea MenosNitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion o... Mostrar Tudo |
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Palavras-Chave: |
Nitrospirillum amazonense; Promoting plant growth. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02478naa a2200265 a 4500
001 2152151
005 2023-03-07
008 2023 bl uuuu u00u1 u #d
022 $a1573-5036
100 1 $aSCHWAB, S.
245 $aQuantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods,$h[electronic resource]
260 $c2023
520 $aNitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion of the mini-setts in bacterial suspension proved to be more efficient than the inoculation methods of applying the suspension onto the post-sprouting substrate or to the lowest lea
653 $aNitrospirillum amazonense
653 $aPromoting plant growth
700 1 $aHIRATA, E. S.
700 1 $aAMARAL, J. C. A.
700 1 $aSILVA, C. G. N.
700 1 $aFERREIRA, J. P.
700 1 $aSILVA, L. V. da
700 1 $aROUWS, J. R. C.
700 1 $aROUWS, L. F. M.
700 1 $aBALDANI, J. I.
700 1 $aREIS, V. M.
773 $tPlant and Soil , Published: 22 February 2023.
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