Data/hora: 03/06/2024 18:07:53

Biblioteca(s): | Embrapa Soja.
Data corrente: | 18/05/2010
Data da última atualização: | 23/01/2017
Autoria: | FREIRE, M. C. M.; OLIVEIRA, L. O. de; ALMEIDA, A. M. de R.; SCHUSTER, I.; MOREIRA, M. A.; LIEBENBERG, M. M.; MIENIE, C. M. S.
Afiliação: | MAÍRA C. M. FREIRE, UFV - Instituto de Biotecnologia Aplicada à Agropecuária; LUIZ O. DE OLIVEIRA, UFV - Instituto de Biotecnologia Aplicada à Agropecuária; ALVARO MANUEL RODRIGUES ALMEIDA, CNPSo; IVAN SHUSTER, Cooperativa Central de Pesquisa Agrícola; MAURÍLIO A. MOREIRA, UFV - Instituto de Biotecnologia Aplicada à Agropecuária; MERION M. LIEBENBERG, ARC-Grain Crops Institute, Potchefstroom, South Africa; CHARLOTTE M. S. MIENIE, ARC-Grain Crops Institute, Potchefstroom, South Africa.
Título: | Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA.
Ano de publicação: | 2008
Fonte/Imprenta: | Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 4, p. 920-931, 2008.
Idioma: | Inglês
Conteúdo: | Phakopsora pachyrhizi has dispersed globally and brought severe economic losses to soybean growers. The fungus has been established in Brazil since 2002 and is found nationwide. To gather information on the temporal and spatial patterns of genetic variation in P. pachyrhizi, we sequenced the nuclear internal transcribed spacer regions (ITS1 and ITS2). Total genomic DNA was extracted using either lyophilized urediniospores or lesions removed from infected leaves sampled from 26 soybean fields in Brazil and one field in South Africa. Cloning prior to sequencing was necessary because direct sequencing of PCR amplicons gave partially unreadable electrophoretograms with peak displacements suggestive of multiple sequences with length polymorphism. Sequences were determined from four clones per field. ITS sequences from African or Asian isolates available from the GenBank were included in the analyses. Independent sequence alignments of the ITS1 and ITS2 datasets identified 27 and 19 ribotypes, respectively. Molecular phylogeographic analyses revealed that ribotypes of widespread distribution in Brazil displayed characteristics of ancestrality and were shared with Africa and Asia, while ribotypes of rare occurrence in Brazil were indigenous. The results suggest P. pachyrhizi found in Brazil as originating from multiple, independent long-distance dispersal events.
Palavras-Chave: | Ferrugem asiática da soja; Fungal disease of plants.
Thesagro: | Fungo; Phakopsora pachyrhizi.
Thesaurus Nal: | Soybean rust.
Categoria do assunto: | --
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (30734-1) - UPC - AP - PP - 11832 -  - 11832 - DIS