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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
30/06/2009 |
Data da última atualização: |
30/04/2010 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
KUBO, K. S.; ASTUA, J. de F.; MACHADO, M. A.; KITAJIMA, E. W. |
Afiliação: |
Karen Sumire Kubo, ESALQ/IAC; JULIANA DE FREITAS ASTUA, CNPMF; Marcos Machado, IAC; Elliot Watanabe Kitajima, ESALQ. |
Título: |
Orchid fleck symptoms may be caused naturally by two different viruses transmitted by Brevipalpus. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Journal of General Plant Pathology, Japan, n. 75, p. 250-255, mai. 2009. |
ISBN: |
ISSN 1345-2630 (Print) 1610-739X (Online) |
DOI: |
10.1007/s10327-009-0167-z |
Idioma: |
Inglês |
Conteúdo: |
Brevipalpus-transmitted viruses (BTV) cause chlorotic, necrotic and/or ringspot lesions in leaves and stems of orchids, citrus, coffee and several other plant species. There are two different types of BTVs, the nuclear and the cytoplasmic, based on maturation locale in the cell and particle morphology. The orchid fleck virus (OFV) is a BTV that infects orchids. Its short rodlike particles are 32 40 nm in diameter, 100 150 nm in length. OFV is found in the nucleus and is associated with intranuclear electronlucent viroplasms. In 1999, transmission electron microscopy analysis revealed a distinct type of virus causing orchid fleck symptoms. The bacilliform particles, 70-80 nm in diameter and 110-120 nm in length, induced electrondense viroplasm inclusions in infected cells and resembled the cytoplasmic type associated with BTV, such as the citrus leprosis virus C. Our objective in the present study was to verify whether the cytoplasmic type virus found in orchids could be amplified using primers for other cytoplasmic BTVs, such as CiLV-C and Solanum violaefolium ringspot virus (SvRSV). Additionally, we aimed to differentiate the two BTVs found in orchids: the nuclear and the cytoplasmic types of OFV using microscopy and molecular and serological tools. This virus was not amplified by the CiLV-C and SvRSV primers, and neither the molecular nor the serological tools available to the OFV diagnosis reacted with it, demonstrating that they are definitely different viruses. |
Palavras-Chave: |
Brevipalpus transmitted virus; Cytoplasmic type; Nuclear type. |
Thesaurus Nal: |
Orchid fleck virus. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02167naa a2200217 a 4500 001 1655738 005 2010-04-30 008 2009 bl --- 0-- u #d 024 7 $a10.1007/s10327-009-0167-z$2DOI 100 1 $aKUBO, K. S. 245 $aOrchid fleck symptoms may be caused naturally by two different viruses transmitted by Brevipalpus. 260 $c2009 520 $aBrevipalpus-transmitted viruses (BTV) cause chlorotic, necrotic and/or ringspot lesions in leaves and stems of orchids, citrus, coffee and several other plant species. There are two different types of BTVs, the nuclear and the cytoplasmic, based on maturation locale in the cell and particle morphology. The orchid fleck virus (OFV) is a BTV that infects orchids. Its short rodlike particles are 32 40 nm in diameter, 100 150 nm in length. OFV is found in the nucleus and is associated with intranuclear electronlucent viroplasms. In 1999, transmission electron microscopy analysis revealed a distinct type of virus causing orchid fleck symptoms. The bacilliform particles, 70-80 nm in diameter and 110-120 nm in length, induced electrondense viroplasm inclusions in infected cells and resembled the cytoplasmic type associated with BTV, such as the citrus leprosis virus C. Our objective in the present study was to verify whether the cytoplasmic type virus found in orchids could be amplified using primers for other cytoplasmic BTVs, such as CiLV-C and Solanum violaefolium ringspot virus (SvRSV). Additionally, we aimed to differentiate the two BTVs found in orchids: the nuclear and the cytoplasmic types of OFV using microscopy and molecular and serological tools. This virus was not amplified by the CiLV-C and SvRSV primers, and neither the molecular nor the serological tools available to the OFV diagnosis reacted with it, demonstrating that they are definitely different viruses. 650 $aOrchid fleck virus 653 $aBrevipalpus transmitted virus 653 $aCytoplasmic type 653 $aNuclear type 700 1 $aASTUA, J. de F. 700 1 $aMACHADO, M. A. 700 1 $aKITAJIMA, E. W. 773 $tJournal of General Plant Pathology, Japan$gn. 75, p. 250-255, mai. 2009.
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