Data/hora: 04/06/2024 14:16:00

Biblioteca(s): | Embrapa Gado de Leite; Embrapa Instrumentação.
Data corrente: | 20/04/2023
Data da última atualização: | 18/07/2023
Autoria: | PEREA, G. N. R.; PAVINATTO, A.; SCHNEIDER, R.; MUNK, M.; BRANDAO, H. de M.; CORREA, D. S.
Afiliação: | Universidade de São Paulo; Universidade Federal de São Carlos; Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; DANIEL SOUZA CORREA, CNPDIA.
Título: | Electrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillin: investigation of morphological features, antibiotic release and antimicrobial properties.
Ano de publicação: | 2023
Fonte/Imprenta: | Journal of Polymer Research, v. 30, 166, 2023.
DOI: | https://doi.org/10.1007/s10965-023-03535-7
Idioma: | Inglês
Conteúdo: | Polymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides, the drug release kinetics study indicates that the nanocarrier system formed by PVP/CHI can release the antibiotics more slowly due to the chemical interactions between CHI and CLO compounds. Our results suggest that drug activity was preserved after encapsulation by electrospinning, and the diference in the inhibition zone diameter was corroborated by drug release mechanism experiments, indicating the system as a potential material to treat infections caused by S. aureus
Palavras-Chave: | Antibiotic release; Electrospinning; Eletrofiação; Mastite; Slow-release.
Thesagro: | Antibiótico; Bovino; Doença Animal; Tratamento.

Categoria do assunto:

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L Ciência Animal e Produtos de Origem Animal

Registro original: | Embrapa Instrumentação (CNPDIA)

Exemplares:

CNPDIA - (18224-1) - UPC - AP - DD - PROCI.23/39 -  - 2023/43 - DIS
CNPGL - (26018-1) - UPC - AP - DD -  -  -  - DIS