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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/09/2012 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FEDERICI, M. T.; PICCHI, S. C.; STUCHI, E. S.; FADEL, A. L.; LAIA, M. L.; LEMOS, M. V. F.; LEMOS, E. G. M. |
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Afiliação: |
MARÍA TERESA FEDERICI, INIA; SIMONE CRISTINA PICCHI, UNESP; EDUARDO SANCHES STUCHI, CNPMF; ANDRÉ LUIZ FADEL, ESALQ; MARCELO LUIZ LAIA, UFVJM; MANOEL VICTOR FRANCO LEMOS, UNESP; ELIANA GERTRUDES MACEDO LEMOS, UNESP. |
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Título: |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Electronic Journal of Biotechnology, 2012. |
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ISSN: |
0717-3458 |
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DOI: |
10.2225/vol15-issue3-fulltext-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increas... Mostrar Tudo |
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Palavras-Chave: |
Microarray. |
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Thesagro: |
Xylella Fastidiosa. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
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001 1933882
005 2023-05-25
008 2012 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $a10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M. T.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCitrus
650 $aXylella Fastidiosa
653 $aMicroarray
700 1 $aPICCHI, S. C.
700 1 $aSTUCHI, E. S.
700 1 $aFADEL, A. L.
700 1 $aLAIA, M. L.
700 1 $aLEMOS, M. V. F.
700 1 $aLEMOS, E. G. M.
773 $tElectronic Journal of Biotechnology, 2012.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
25/04/2000 |
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Data da última atualização: |
06/10/2022 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
SEGOVIA, J. F. O.; ANDRIOLO, J. L.; BURIOL, G. A.; SCHNEIDER, F. M. |
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Afiliação: |
JORGE FEDERICO ORELLANA SEGOVIA, CPAF-AP; JERÔNIMO LUIZ ANDRIOLO, UNIVERSIDADE FEDERAL DE SANTA MARIA; GALILEO ADELI BURIOL, UNIVERSIDADE FEDERAL DE SANTA MARIA; FLÁVIO MIGUEL SCHNEIDER, UNIVERSIDADE FEDERAL DE SANTA MARIA. |
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Título: |
Influência de uma estufa coberta de polietileno transparente no crescimento de alface. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Macapá: Embrapa Amapá, 1999. |
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Páginas: |
16 p. |
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Série: |
(Embrapa Amapá. Boletim de pesquisa, 27). |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho teve como objetivo avaliar o desempenho de três cultivares de alface cultivadas em ambiente natural e no interior de estufa coberta com polietileno transparente, durante o inverno. |
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Palavras-Chave: |
Lettuces. |
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Thesagro: |
Alface; Hortaliça; Lactuca Sativa. |
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Thesaurus NAL: |
horticulture. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97531/1/CPAF-AP-1999-Influencia-estufa.pdf
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Marc: |
LEADER 00829nam a2200229 a 4500
001 1344625
005 2022-10-06
008 1999 bl uuuu u0uu1 u #d
100 1 $aSEGOVIA, J. F. O.
245 $aInfluência de uma estufa coberta de polietileno transparente no crescimento de alface.
260 $aMacapá: Embrapa Amapá$c1999
300 $a16 p.
490 $a(Embrapa Amapá. Boletim de pesquisa, 27).
520 $aEste trabalho teve como objetivo avaliar o desempenho de três cultivares de alface cultivadas em ambiente natural e no interior de estufa coberta com polietileno transparente, durante o inverno.
650 $ahorticulture
650 $aAlface
650 $aHortaliça
650 $aLactuca Sativa
653 $aLettuces
700 1 $aANDRIOLO, J. L.
700 1 $aBURIOL, G. A.
700 1 $aSCHNEIDER, F. M.
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