Data/hora: 04/06/2024 02:29:05

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	05/04/2005	
Data da última atualização: 	05/04/2005	
Autoria: 	REIS, V. M.; SANTOS, P. E. -de los; TENORIO-SALGADO, S.; VOGEL, J.; STOFFELS, M.; GUYON, S.; MAVINGUI, P.; BALDANI, V. L. D.; SCHMID, M.; BALDANI, J. I.; BALANDREAU, J.; HARTMANN, A.; CABALLERO-MELLADO, J.	
Título: 	Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium.	
Ano de publicação: 	2004	
Fonte/Imprenta: 	International Journal of Systematic and Evolutionary Microbiology, Reading, v. 54, p. 2155-2162, 2004.	
Idioma: 	Inglês	
Conteúdo: 	In an ecological survey of nitrogen-fixing bacteria isolated from the rhizosphere and as endophytes of sugarce, maize and teosinte plants in Brazil, Mexico and South Africa, a new phylogenetically homogeneous group of N2-fixing bacteria was identified within the genus Burkholderia. This polyphasic taxonomic study included microscopic and colony morphology, API 20NE tests and growth on different culture media at different pH and temperatures, as well as carbon source assimilation tests and whole-cell protein pattern analysis. Analysis of 16S rRNA gene sequences showed 99.2-99.9% similarity within the novel species and 97.2% similarity to the closet related species, Burkholderia sacchari. The novel species was composed of four distinct amplified 16S rDNA restriction analysis groups. The DNA-DNA reassociation values within the novel species were greater than 70% and less than 42% for the closet related species, B. sacchari. Based on these results and on many phenotypic characteristics, a novel N2-fixing species is proposed for the genus Burkholderia, Burkholderia tropica sp. nov., with the type strain Ppe8T (=ATCC BAA-831T=DSM 15359T). B. tropica was isolated from plants grown in geographical regions with climates ranging from temperates subhumid to hot humid.	
Palavras-Chave: 	Bactéria diazotrófica; Biological nitrogen fixation; BNF; Burkholderia tropica; Cana-de-açúcar; Deoxyribonucleic acid; Diazotrophic bacteria; FBN; Fixação biológica de nitrogênio; Growth; Phenotypes; Protein; Sugar cane.	
Thesagro: 	Ácido Nucléico; Crescimento; DNA; Fenótipo; Proteína; Sideróforo.	
Thesaurus Nal: 	nucleic acids; siderophores.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (32249-1) - UMT - SP - -- -  -  - 0022790 - DIS