Data/hora: 05/06/2024 01:24:40

Biblioteca(s): | Embrapa Trigo.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 2
Data corrente: | 10/12/2014
Data da última atualização: | 01/02/2015
Autoria: | SCAGLIUSI, S. M. M.
Afiliação: | SANDRA MARIA MANSUR SCAGLIUSI, CNPT.
Título: | Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.).
Ano de publicação: | 2014
Fonte/Imprenta: | Australian Journal of Crop Science, v. 8, n. 6, p. 887-894, 2014.
ISSN: | 1835-2707
Idioma: | Inglês
Conteúdo: | The objective of this study was to establish an isolated microspore culture (IMC) protocol in wheat (Triticum aestivum L.) for use in genetic studies and to evaluate its potential for routine use in the Brazilian Wheat Breeding Program at Embrapa Wheat. Important steps of the method were identified and plant physiology of microspore mother plants and ovary co-culture were considered as key factors for effective establishment. Three Brazilian wheat genotypes were tested (Toropi, BRS 194 and F1 wheat cross 020037 × 020062), and two other genotypes were used as controls (Bobwhite and Fielder). Spikes containing uninucleated microspores were subjected to cold pretreatment (4°C) for 21 days in the dark. Number of embryos, green and albino plants were recorded for each genotype. The method was successfully established, and several fertile green plants were produced by using tissue culture and responsive controls. However, the results greatly differed among Brazilian wheat genotypes, suggesting a strong genotype-dependent effect. Microspore induction medium alone did not promote embryogenesis; ovary co-culture was a necessary step for embryo development and green plant formation, for all genotypes. The F1 wheat cross (020037 × 020062) produced a total of 85 green plants (out of 108 spikes), 64% of which were spontaneous diploids. BRS 194 produced many embryos, exhibiting a good androgenic response, but only a few grew into green plants. Toropi behaved as a recalcitrant genotype, and zero plants were produced. To our knowledge, this is the first report on wheat IMC from Brazilian genotypes resulting in androgenic embryogenesis and plant regeneration.
Palavras-Chave: | Brasil; Duplo haplóide; Microspore culture; Wheat breeding.
Thesagro: | Melhoramento Genético Vegetal; Trigo.
Thesaurus Nal: | androgenesis.
Categoria do assunto: | G Melhoramento Genético
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/113534/1/2014-Australian-Journal-of-Crop-Science-v8n6p887.pdf
Registro original: | Embrapa Trigo (CNPT)
Exemplares: | CNPT - (42702-1) - UPC - AP - DD -  -  -  - DIS