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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
07/03/2012 |
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Data da última atualização: |
21/10/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; UNESP/JABOTICABAL. |
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Título: |
Effects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO, 2012 |
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Páginas: |
p. 119 |
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Idioma: |
Português |
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Conteúdo: |
Enucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable. MenosEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated... Mostrar Tudo |
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Palavras-Chave: |
Demecolcine; Meiotic cell cycle. |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03762nam a2200217 a 4500
001 1917825
005 2015-10-21
008 2012 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aEffects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation.
260 $aIn: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO$c2012
300 $ap. 119
520 $aEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable.
650 $aoocytes
653 $aDemecolcine
653 $aMeiotic cell cycle
700 1 $aOLIVEIRA, C. S.
700 1 $aTETZNER, T. A. D.
700 1 $aLIMA, M. R. de
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/12/2021 |
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Data da última atualização: |
09/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
SANTOS, V. O. dos; BOMFIM, M. A. D.; SANTOS, S. F. dos; TONUCCI, R. G.; CAVALCANTE, A. C. R.; CHAVES, A. K. de L.; PEREIRA, P. L. |
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Afiliação: |
VALCICLEIDE OLIVEIRA DOS SANTOS, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; MARCO AURELIO DELMONDES BOMFIM, CNPC; SUELI FREITAS DOS SANTOS; RAFAEL GONCALVES TONUCCI, CNPC; ANA CLARA RODRIGUES CAVALCANTE, CNPC; ANA KARINA DE LIMA CHAVES, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; PATRÍCIO LEANDRO PEREIRA, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil. |
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Título: |
Efeito do cultivar e do grau de maturidade dos grãos sobre os espectros NIR de silagens de sorgo. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
PUBVET, v. 15, n. 12, a1000, p. 1-10, dez. 2021. |
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DOI: |
https://doi.org/10.31533/pubvet.v15n12a1000.1-10 |
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Idioma: |
Português |
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Conteúdo: |
Resumo. Objetivou-se, avaliar a similaridade espectral entre cultivares de sorgo em diferentes estádios de maturação dos grãos, visando o desenvolvimento de modelos utilizando espectroscopia de refletância no infravermelho próximo (NIRS). Para a ensilagem utilizou-se os sorgos BRS 658 e BRS Ponta Negra, em mini silos de PVC, nos estádios de maturação dos grãos leitoso, pastoso, farináceo e duro. As variáveis foram estudadas pela técnica de Análise de Componentes Principais (PCA), e classificação usando a Modelagem Independente por Analogia de Classe (SIMCA) utilizando o software Unscrambler. A similaridade de amostras da silagem do BRS 658 com o banco de dados do BRS Ponta negra foi de 5% e do BRS Ponta Negra em relação as do BRS 658 a similaridade foi de 65%. Os dados demonstram grande similaridade do BRS Ponta Negra entre os estádios dos grãos leitoso e pastoso. A análise da PCA, demonstrou uma semelhança entre as amostras no estádio leitoso das duas cultivares, no estádio leitoso com pastoso, farináceo e duro. A análise exploratória indica que é importante considerar a cultivar e os estádios de maturidade dos grãos para o desenvolvimento de modelos NIR de silagem de sorgo. A análise dos espectros NIR das cultivares BRS 658 e BRS Ponta Negra, bem como dos estádios de maturidade no momento da ensilagem indicam que as dissimilaridades observadas agregam variabilidade ao modelo, mas que, ao mesmo tempo, não são tão significativas a ponto de sugerirem desenvolvimento de modelos separados, permitindo a fusão dos dados para desenvolvimento de um único modelo NIR. [Effect of cultivar and grain maturity on NIR spectra of sorghum silages]. Abstract. The objective of this study was to evaluate the spectral similarity between sorghum cultivars at different stages of grain maturation, aiming at the development of models using near infrared reflectance spectroscopy (NIRS). For ensiling, sorghums BRS 658 and BRS Ponta Negra were used in PVC mini silos, in the stages of maturation of milky, pasty, farinaceous and hard grains. The variables were studied using the Principal Component Analysis (PCA) technique, and classification using Independent Class Analogy Modeling (SIMCA) using the Unscrambler software. The similarity of silage samples from BRS 658 with the BRS Ponta Negra database was 5% and from BRS Ponta Negra to BRS 658 the similarity was 65%. The data show great similarity of BRS Ponta Negra between the milky and pasty grain stages. The PCA analysis showed a similarity between the samples in the milky stages of the two cultivars, in the milky stage with pasty, farinaceous and hard. The exploratory analysis indicates that it is important to consider the cultivar and the grain maturity stages for the development of NIR sorghum silage models. The analysis of the NIR spectra of the cultivars BRS 658 and BRS Ponta Negra, as well as the stages of maturity at the time of ensiling indicate that the dissimilarities observed add variability to the model, but that, at the same time, they are not so significant as to suggest development of separate models, allowing the merging of data to develop a single NIR model. MenosResumo. Objetivou-se, avaliar a similaridade espectral entre cultivares de sorgo em diferentes estádios de maturação dos grãos, visando o desenvolvimento de modelos utilizando espectroscopia de refletância no infravermelho próximo (NIRS). Para a ensilagem utilizou-se os sorgos BRS 658 e BRS Ponta Negra, em mini silos de PVC, nos estádios de maturação dos grãos leitoso, pastoso, farináceo e duro. As variáveis foram estudadas pela técnica de Análise de Componentes Principais (PCA), e classificação usando a Modelagem Independente por Analogia de Classe (SIMCA) utilizando o software Unscrambler. A similaridade de amostras da silagem do BRS 658 com o banco de dados do BRS Ponta negra foi de 5% e do BRS Ponta Negra em relação as do BRS 658 a similaridade foi de 65%. Os dados demonstram grande similaridade do BRS Ponta Negra entre os estádios dos grãos leitoso e pastoso. A análise da PCA, demonstrou uma semelhança entre as amostras no estádio leitoso das duas cultivares, no estádio leitoso com pastoso, farináceo e duro. A análise exploratória indica que é importante considerar a cultivar e os estádios de maturidade dos grãos para o desenvolvimento de modelos NIR de silagem de sorgo. A análise dos espectros NIR das cultivares BRS 658 e BRS Ponta Negra, bem como dos estádios de maturidade no momento da ensilagem indicam que as dissimilaridades observadas agregam variabilidade ao modelo, mas que, ao mesmo tempo, não são tão significativas a ponto de sugerirem desenvolvimento de modelo... Mostrar Tudo |
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Palavras-Chave: |
Análise multivariada; Espectroscopia; NIRS; SIMCA. |
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Thesagro: |
Gramínea Forrageira; Sorgo Forrageiro. |
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Thesaurus NAL: |
Analytical methods; Forage grasses; Multivariate analysis; Near-infrared spectroscopy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 04189naa a2200325 a 4500
001 2137374
005 2021-12-09
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.31533/pubvet.v15n12a1000.1-10$2DOI
100 1 $aSANTOS, V. O. dos
245 $aEfeito do cultivar e do grau de maturidade dos grãos sobre os espectros NIR de silagens de sorgo.$h[electronic resource]
260 $c2021
520 $aResumo. Objetivou-se, avaliar a similaridade espectral entre cultivares de sorgo em diferentes estádios de maturação dos grãos, visando o desenvolvimento de modelos utilizando espectroscopia de refletância no infravermelho próximo (NIRS). Para a ensilagem utilizou-se os sorgos BRS 658 e BRS Ponta Negra, em mini silos de PVC, nos estádios de maturação dos grãos leitoso, pastoso, farináceo e duro. As variáveis foram estudadas pela técnica de Análise de Componentes Principais (PCA), e classificação usando a Modelagem Independente por Analogia de Classe (SIMCA) utilizando o software Unscrambler. A similaridade de amostras da silagem do BRS 658 com o banco de dados do BRS Ponta negra foi de 5% e do BRS Ponta Negra em relação as do BRS 658 a similaridade foi de 65%. Os dados demonstram grande similaridade do BRS Ponta Negra entre os estádios dos grãos leitoso e pastoso. A análise da PCA, demonstrou uma semelhança entre as amostras no estádio leitoso das duas cultivares, no estádio leitoso com pastoso, farináceo e duro. A análise exploratória indica que é importante considerar a cultivar e os estádios de maturidade dos grãos para o desenvolvimento de modelos NIR de silagem de sorgo. A análise dos espectros NIR das cultivares BRS 658 e BRS Ponta Negra, bem como dos estádios de maturidade no momento da ensilagem indicam que as dissimilaridades observadas agregam variabilidade ao modelo, mas que, ao mesmo tempo, não são tão significativas a ponto de sugerirem desenvolvimento de modelos separados, permitindo a fusão dos dados para desenvolvimento de um único modelo NIR. [Effect of cultivar and grain maturity on NIR spectra of sorghum silages]. Abstract. The objective of this study was to evaluate the spectral similarity between sorghum cultivars at different stages of grain maturation, aiming at the development of models using near infrared reflectance spectroscopy (NIRS). For ensiling, sorghums BRS 658 and BRS Ponta Negra were used in PVC mini silos, in the stages of maturation of milky, pasty, farinaceous and hard grains. The variables were studied using the Principal Component Analysis (PCA) technique, and classification using Independent Class Analogy Modeling (SIMCA) using the Unscrambler software. The similarity of silage samples from BRS 658 with the BRS Ponta Negra database was 5% and from BRS Ponta Negra to BRS 658 the similarity was 65%. The data show great similarity of BRS Ponta Negra between the milky and pasty grain stages. The PCA analysis showed a similarity between the samples in the milky stages of the two cultivars, in the milky stage with pasty, farinaceous and hard. The exploratory analysis indicates that it is important to consider the cultivar and the grain maturity stages for the development of NIR sorghum silage models. The analysis of the NIR spectra of the cultivars BRS 658 and BRS Ponta Negra, as well as the stages of maturity at the time of ensiling indicate that the dissimilarities observed add variability to the model, but that, at the same time, they are not so significant as to suggest development of separate models, allowing the merging of data to develop a single NIR model.
650 $aAnalytical methods
650 $aForage grasses
650 $aMultivariate analysis
650 $aNear-infrared spectroscopy
650 $aGramínea Forrageira
650 $aSorgo Forrageiro
653 $aAnálise multivariada
653 $aEspectroscopia
653 $aNIRS
653 $aSIMCA
700 1 $aBOMFIM, M. A. D.
700 1 $aSANTOS, S. F. dos
700 1 $aTONUCCI, R. G.
700 1 $aCAVALCANTE, A. C. R.
700 1 $aCHAVES, A. K. de L.
700 1 $aPEREIRA, P. L.
773 $tPUBVET$gv. 15, n. 12, a1000, p. 1-10, dez. 2021.
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