Data/hora: 06/06/2024 13:01:20

Biblioteca(s): | Embrapa Agroenergia; Embrapa Agroindústria de Alimentos.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 2
Data corrente: | 22/09/2014
Data da última atualização: | 15/02/2016
Autoria: | SANTOS, R. dos S.; MURUCI, L. N. M.; SANTOS, L. O.; ANTONIASSI, R.; SILVA, J. P. L. da; DAMASO, M. C. T.
Afiliação: | Regiane Ribeiro dos Santos, Universidade Federal Rural do Rio de Janeiro; Livia Nolasco Macedo Muruci, Universidade Federal Rural do Rio de Janeiro; Lucielen Oliveira Santos, Universidade Federal Rural do Rio de Janeiro; ROSEMAR ANTONIASSI, CTAA; JANINE PASSOS LIMA DA SILVA, CTAA; MONICA CARAMEZ TRICHES DAMASO, CNPAE.
Título: | Characterization of different oil soapstocks and their application in the lipase production by Aspergillus niger under solid state fermentation.
Ano de publicação: | 2014
Fonte/Imprenta: | Journal of Food and Nutrition Research, v. 2, n. 9, p. 561-566, 2014.
DOI: | 10.12691/jfnr-2-9-6
Idioma: | Inglês
Conteúdo: | Lipases are enzymes that catalyze the hydrolysis of the ester linkage in a triacylglycerol oil-water interface, while in non-aqueous environments they catalyze reverse reactions (esterification, transesterification and interesterification). They can be produced by a solid state fermentation (SSF) process adding value to agro-industrial residues as alkaline soapstocks that can be used as a lipid substrate for lipase production. The objective of this study was to characterize the soapstocks of canola, sunflower and corn and use these byproducts for fungal lipase production using the mutant strain 11T53A14 of Aspergillus niger. The work was carried out following 24-1 and 23 experimental designs in the presence and absence of inducers, respectively, aiming to evaluate the influence of some variables in the lipase production. The production by SSF was carried out in aerated columns incubated at 32°C. Among the soapstocks evaluated, the sunflower soapstock was the best inducer for enzyme production, with 201 U/gdm (gram of dry medium) using 108 spores/gm (gram of medium) inoculated on wheat bran humidified with 80 mL of liquid (ammonium sulfate solution) that corresponds to a final concentration of 0.5% nitrogen, and 3% inducer. The best lipase activity obtained without soapstock was 182 U/gdm under the same processing conditions as the experiment using soapstock. Although the results with the highest lipase activity with sunflower soapstock and without inducer are close, the Tukey test showed a significant difference of these results, which proves the importance of lipid substrate to improve the lipase activity.
Palavras-Chave: | Column reactor; Fermentação em estado sólido; Filamentous fungus; Fungos filamentosos; Lipases; Reator de coluna; Resíduos agroindustriais.
Thesagro: | Lípase.
Thesaurus Nal: | solid state fermentation.

Categoria do assunto:

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X Pesquisa, Tecnologia e Engenharia

URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/109353/1/2014-034.pdf
Registro original: | Embrapa Agroindústria de Alimentos (CTAA)

Exemplares:

CNPAE - (2499-1) - UPC - AP - DD -  -  -  - DIS
CTAA - (12308-1) - UPC - AP - PP - 2014/034 -  - 2014.00034 - DIS