Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
20/05/2005 |
Data da última atualização: |
12/09/2005 |
Autoria: |
SANTOS, M. de O.; ROMANO, E.; YOTOKO, K. S. C.; TINOCO, M. L. P.; DIAS, B. B. A.; ARAGÃO, F. J. L. |
Título: |
Characterization of the cacao somatic embryogenesis receptor-like kinase (SERK) gene expressed during somatic embryogenesis. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Plant Science, Limerick, v. 168, n. 3, p. 723-729, Mar. 2005. |
Idioma: |
Inglês |
Conteúdo: |
Somatic embryogenesis is a useful tool for plant propagation and consists of a good model for embryo development studies. During somatic embryogenesis, some morphological and biochemical changes occur in response to alterations in gene expression patterns. The somatic embryogenesis receptor kinase (SERK) gene is an important element related to this process. In this work we have focused on the function of SERK in Theobroma cacao. Our main questions were whether this gene exists in this plant with another arrangement; if it is functional and, in a positive case, in which tissues it acts. We have found only one copy of SERK that is functional and analyzed its expression in a series of tissues. The phylogenetic tree showed that the available sequences of SERK are highly conserved and a considerable number of species contain different sequences of SERK, confirming it as a gene family with variant repeats.
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Palavras-Chave: |
Clonagem de genes; Expressão gênica; Família gênica; Filogenia molecular; RT-PCR; Thebroma cacao. |
Categoria do assunto: |
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Marc: |
LEADER 01675naa a2200253 a 4500 001 1185674 005 2005-09-12 008 2005 bl --- 0-- u #d 100 1 $aSANTOS, M. de O. 245 $aCharacterization of the cacao somatic embryogenesis receptor-like kinase (SERK) gene expressed during somatic embryogenesis. 260 $c2005 520 $aSomatic embryogenesis is a useful tool for plant propagation and consists of a good model for embryo development studies. During somatic embryogenesis, some morphological and biochemical changes occur in response to alterations in gene expression patterns. The somatic embryogenesis receptor kinase (SERK) gene is an important element related to this process. In this work we have focused on the function of SERK in Theobroma cacao. Our main questions were whether this gene exists in this plant with another arrangement; if it is functional and, in a positive case, in which tissues it acts. We have found only one copy of SERK that is functional and analyzed its expression in a series of tissues. The phylogenetic tree showed that the available sequences of SERK are highly conserved and a considerable number of species contain different sequences of SERK, confirming it as a gene family with variant repeats. 653 $aClonagem de genes 653 $aExpressão gênica 653 $aFamília gênica 653 $aFilogenia molecular 653 $aRT-PCR 653 $aThebroma cacao 700 1 $aROMANO, E. 700 1 $aYOTOKO, K. S. C. 700 1 $aTINOCO, M. L. P. 700 1 $aDIAS, B. B. A. 700 1 $aARAGÃO, F. J. L. 773 $tPlant Science, Limerick$gv. 168, n. 3, p. 723-729, Mar. 2005.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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