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Registros recuperados : 188 | |
31. | | SANTOS, M. R. A. dos; FERREIRA, M. das G. R.; SARUBO, V. Balanço hormonal de 2,4-D, 2iP e AIB para calogênese em café conilon (Coffea canephora PIERRE). In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 6., 2009, Vitória. Inovação científica, competitividade e mudanças climáticas : anais. Vitória: Consórcio Pesquisa Café, 2009. Biblioteca(s): Embrapa Rondônia. |
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32. | | SANTOS, M. R. A. dos; FERREIRA, M. das G. R.; SARUBO, V. Balanço hormonal de 2,-D, 2iP e AIB para calogênese em café conilon (Coffea canephora Pierre). In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4., 2009, Aracaju. Ciência, inovação e sustentabilidade: anais. Aracaju: Embrapa Tabuleiros Costeiros, 2009. Biblioteca(s): Embrapa Rondônia. |
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Registros recuperados : 188 | |
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Registro Completo
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
30/12/2021 |
Data da última atualização: |
01/01/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SOUZA, J. A. de; SANTOS, M. R. A. dos. |
Afiliação: |
JAQUELINE ANDRUCHEVITZ DE SOUZA, FIMCA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO. |
Título: |
In vitro propagation of Piper hispidum by somatic embryogenesis in leaf explants. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
International Journal of Development Research, v. 11, n. 12, p. 52392-52393, Dec. 2021. |
ISSN: |
2230-9926 |
DOI: |
https://doi.org/10.37118/ijdr.23567.12.2021 |
Idioma: |
Inglês |
Conteúdo: |
A protocol for regeneration of P. hispidum through somatic embryogenesis in leaf explants is described. Under aseptic conditions, the leaves were cut into 1.0 cm2 explants. These explants were inoculated into test tubes with 10.0 mL of an Murashige and Skoog (MS) basal culture medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and a factorial combination of the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-Benzylaminopurine (BA), both at 0.0, 1.0, 2.0, 3.0 and 4.0 mg L-1 , totalizing 25 treatments. The pH was adjusted to 5.8 and the medium autoclaved at 121°C for 20 minutes. After 75 days, the average number of cotyledonary somatic embryos in each explant was recorded.Maximum number of 9.2 cotyledonary embryos per explant was observed with the combination of 2.0 mg L-1 BA + 2.0 mg L-1 2,4-D. The subculture of the cotyledonary embryos to a medium without growth regulators resulted in 95% conversion into plantlets, which were acclimatized with 100% survival. |
Palavras-Chave: |
Cotyledonary embryos; Embriogênese somática; Protocolo de regeneração. |
Thesagro: |
Cultura de Tecido; Cultura In Vitro; Explante; Folha; Propagação Vegetativa; Reprodução Vegetal. |
Thesaurus NAL: |
Explants; Genetic techniques and protocols; In vitro culture; Leaves; Piper hispidum; Plant reproduction; Somatic embryogenesis; Tissue culture; Vegetative propagation. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229831/1/cpafro-18666.pdf
|
Marc: |
LEADER 02157naa a2200373 a 4500 001 2138599 005 2022-01-01 008 2021 bl uuuu u00u1 u #d 022 $a2230-9926 024 7 $ahttps://doi.org/10.37118/ijdr.23567.12.2021$2DOI 100 1 $aSOUZA, J. A. de 245 $aIn vitro propagation of Piper hispidum by somatic embryogenesis in leaf explants.$h[electronic resource] 260 $c2021 520 $aA protocol for regeneration of P. hispidum through somatic embryogenesis in leaf explants is described. Under aseptic conditions, the leaves were cut into 1.0 cm2 explants. These explants were inoculated into test tubes with 10.0 mL of an Murashige and Skoog (MS) basal culture medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and a factorial combination of the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-Benzylaminopurine (BA), both at 0.0, 1.0, 2.0, 3.0 and 4.0 mg L-1 , totalizing 25 treatments. The pH was adjusted to 5.8 and the medium autoclaved at 121°C for 20 minutes. After 75 days, the average number of cotyledonary somatic embryos in each explant was recorded.Maximum number of 9.2 cotyledonary embryos per explant was observed with the combination of 2.0 mg L-1 BA + 2.0 mg L-1 2,4-D. The subculture of the cotyledonary embryos to a medium without growth regulators resulted in 95% conversion into plantlets, which were acclimatized with 100% survival. 650 $aExplants 650 $aGenetic techniques and protocols 650 $aIn vitro culture 650 $aLeaves 650 $aPiper hispidum 650 $aPlant reproduction 650 $aSomatic embryogenesis 650 $aTissue culture 650 $aVegetative propagation 650 $aCultura de Tecido 650 $aCultura In Vitro 650 $aExplante 650 $aFolha 650 $aPropagação Vegetativa 650 $aReprodução Vegetal 653 $aCotyledonary embryos 653 $aEmbriogênese somática 653 $aProtocolo de regeneração 700 1 $aSANTOS, M. R. A. dos 773 $tInternational Journal of Development Research$gv. 11, n. 12, p. 52392-52393, Dec. 2021.
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