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| Registros recuperados : 188 | |
| Registros recuperados : 188 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
26/11/2020 |
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Data da última atualização: |
26/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS, M. R. A. dos; PAZ, E. S. |
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Afiliação: |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; Universidade Federal de Rondonia. |
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Título: |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
International Journal of Development Research, v. 10, n. 11, p. 41853-41856, Nov. 2020. |
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ISSN: |
2230-9926 |
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DOI: |
10.37118/ijdr.20362.11.2020 |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combinations. The percentage of callus induction (%CI), the explant area covered by callus cells (ACCC) and the fresh weight of the calluses were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The treatment that resulted in the highest %CI, ACCC and callus weight was 1.5 mg L-1 2,4-D. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 39th day of cultivation. |
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Palavras-Chave: |
Amazônia Ocidental; Callogenesis; Capsicum frutescens cv Stromboli; Célula foliar; Deceleration phase; Embrapa Rondônia; Fase de desaceleração; Growth curves; Leaf cells; Metabólicos secundários; Porto Velho (RO); Protocolo de diferenciação celular; Rondônia; Western Amazon. |
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Thesagro: |
Calogênese; Cultura In Vitro; Curva de Crescimento. |
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Thesaurus NAL: |
Callus formation; Cell dedifferentiation; In vitro culture; Secondary metabolites. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218277/1/cpafro-18474.pdf
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Marc: |
LEADER 02684naa a2200409 a 4500
001 2127111
005 2020-11-26
008 2020 bl uuuu u00u1 u #d
022 $a2230-9926
024 7 $a10.37118/ijdr.20362.11.2020$2DOI
100 1 $aSANTOS, M. R. A. dos
245 $aDedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli.$h[electronic resource]
260 $c2020
520 $aIn vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combinations. The percentage of callus induction (%CI), the explant area covered by callus cells (ACCC) and the fresh weight of the calluses were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The treatment that resulted in the highest %CI, ACCC and callus weight was 1.5 mg L-1 2,4-D. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 39th day of cultivation.
650 $aCallus formation
650 $aCell dedifferentiation
650 $aIn vitro culture
650 $aSecondary metabolites
650 $aCalogênese
650 $aCultura In Vitro
650 $aCurva de Crescimento
653 $aAmazônia Ocidental
653 $aCallogenesis
653 $aCapsicum frutescens cv Stromboli
653 $aCélula foliar
653 $aDeceleration phase
653 $aEmbrapa Rondônia
653 $aFase de desaceleração
653 $aGrowth curves
653 $aLeaf cells
653 $aMetabólicos secundários
653 $aPorto Velho (RO)
653 $aProtocolo de diferenciação celular
653 $aRondônia
653 $aWestern Amazon
700 1 $aPAZ, E. S.
773 $tInternational Journal of Development Research$gv. 10, n. 11, p. 41853-41856, Nov. 2020.
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Embrapa Rondônia (CPAF-RO) |
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