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Registro Completo |
Biblioteca(s): |
Embrapa Amapá. |
Data corrente: |
20/08/2007 |
Data da última atualização: |
04/10/2022 |
Autoria: |
MEIRELLES, P. R. de L.; MOCHIUTTI, S. |
Afiliação: |
PAULO ROBERTO DE LIMA MEIRELLES, CPAF-AP; SILAS MOCHIUTTI, CPAF-AP. |
Título: |
Avaliação do potencial forrageiro de variedades de cana-de-açúcar no Amapá. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 36., 1999, Porto Alegre. [Anais]. Porto Alegre: SBZ, 1999. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Produção de forragem; Volumoso. |
Thesagro: |
Matéria Seca; Proteína Bruta. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00593naa a2200181 a 4500 001 1344514 005 2022-10-04 008 1999 bl uuuu u00u1 u #d 100 1 $aMEIRELLES, P. R. de L. 245 $aAvaliação do potencial forrageiro de variedades de cana-de-açúcar no Amapá. 260 $c1999 300 $c1 CD-ROM. 650 $aMatéria Seca 650 $aProteína Bruta 653 $aProdução de forragem 653 $aVolumoso 700 1 $aMOCHIUTTI, S. 773 $tIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 36., 1999, Porto Alegre. [Anais]. Porto Alegre: SBZ, 1999.
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
26/10/2010 |
Data da última atualização: |
30/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
PIROVANI, C. P.; SANTIAGO, A. da S.; SANTOS, L. S. dos; MICHELI, F.; MARGIS, R.; GESTEIRA, A. da S.; ALVIM, F. C.; PEREIRA, G. A. G.; CASCARDO, J. C. de M. |
Afiliação: |
Carlos Priminho Pirovani, UESC; André da Silva Santiago, UESC; Lívia Santana dos Santos, UESC; Fabienne Micheli, CIRAD; Rogério Margis, UFRGS; ABELMON DA SILVA GESTEIRA, CNPMF; Fátima Cerqueira Alvim, UESC; Gonçalo Amarante Guimarães Pereira, UNICAMP; Júlio Cézar de Mattos Cascardo, UESC. |
Título: |
Theobroma cacao cystatins impair Moniliophthora perniciosa mycelial growth and are involved in postponing cell death symptoms. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Planta, Heidelberg, v. 232, n. 6, p. 1485–1497, 2010. |
ISSN: |
0032-0935 |
DOI: |
DOI 10.1007/s00425-010-1272-0 |
Idioma: |
Inglês |
Notas: |
Disponível em: . Acesso em 26 out. 2010. |
Conteúdo: |
Three cystatin open reading frames named TcCys1, TcCys2 and TcCys3 were identified in cDNA libraries from compatible interactions between Theobroma cacao (cacao) and Moniliophthora perniciosa. In addition, an ORF named TcCys4 was identified in the cDNA library of the incompatible interaction. The cDNAs encoded conceptual proteins with 209, 127, 124, and 205 amino acid residues, with a deduced molecular weight of 24.3, 14.1, 14.3 and 22.8 kDa, respectively. His-tagged recombinant proteins were purified from Escherichia coli expression, and showed inhibitory activities against M. perniciosa. The four recombinant cystatins exhibited Ki values against papain in the range of 152–221 nM. Recombinant TcCYS3 and TcCYS4 immobilized in CNBr–Sepharose were efficient to capture M. perniciosa proteases from culture media. Polyclonal antibodies raised against the recombinant TcCYS4 detected that the endogenous protein was more abundant in young cacao tissues, when compared with mature tissues. A *85 kDa cacao multicystatin induced by M. perniciosa inoculation, MpNEP (necrosis and ethylene-inducing protein) and M. perniciosa culture supernatant infiltration were detected by anti-TcCYS4 antibodies in cacao young tissues. A direct role of the cacao cystatins in the defense against this phytopathogen was proposed, as well as its involvement in the development of symptoms of programmed cell death. |
Palavras-Chave: |
Cystatin; Cysteine protease inhibitor; Programmed cell death; Vassoura-de-bruxa; Witches’ broom. |
Thesagro: |
Cacau; Doença de Planta; Fungo; Theobroma Cacao. |
Thesaurus NAL: |
Moniliophthora; Moniliophthora perniciosa. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02635naa a2200385 a 4500 001 1865253 005 2017-05-30 008 2010 bl uuuu u00u1 u #d 022 $a0032-0935 024 7 $aDOI 10.1007/s00425-010-1272-0$2DOI 100 1 $aPIROVANI, C. P. 245 $aTheobroma cacao cystatins impair Moniliophthora perniciosa mycelial growth and are involved in postponing cell death symptoms. 260 $c2010 500 $aDisponível em: <http://www.springerlink.com/content/c41483451417h040/fulltext.pdf>. Acesso em 26 out. 2010. 520 $aThree cystatin open reading frames named TcCys1, TcCys2 and TcCys3 were identified in cDNA libraries from compatible interactions between Theobroma cacao (cacao) and Moniliophthora perniciosa. In addition, an ORF named TcCys4 was identified in the cDNA library of the incompatible interaction. The cDNAs encoded conceptual proteins with 209, 127, 124, and 205 amino acid residues, with a deduced molecular weight of 24.3, 14.1, 14.3 and 22.8 kDa, respectively. His-tagged recombinant proteins were purified from Escherichia coli expression, and showed inhibitory activities against M. perniciosa. The four recombinant cystatins exhibited Ki values against papain in the range of 152–221 nM. Recombinant TcCYS3 and TcCYS4 immobilized in CNBr–Sepharose were efficient to capture M. perniciosa proteases from culture media. Polyclonal antibodies raised against the recombinant TcCYS4 detected that the endogenous protein was more abundant in young cacao tissues, when compared with mature tissues. A *85 kDa cacao multicystatin induced by M. perniciosa inoculation, MpNEP (necrosis and ethylene-inducing protein) and M. perniciosa culture supernatant infiltration were detected by anti-TcCYS4 antibodies in cacao young tissues. A direct role of the cacao cystatins in the defense against this phytopathogen was proposed, as well as its involvement in the development of symptoms of programmed cell death. 650 $aMoniliophthora 650 $aMoniliophthora perniciosa 650 $aCacau 650 $aDoença de Planta 650 $aFungo 650 $aTheobroma Cacao 653 $aCystatin 653 $aCysteine protease inhibitor 653 $aProgrammed cell death 653 $aVassoura-de-bruxa 653 $aWitches’ broom 700 1 $aSANTIAGO, A. da S. 700 1 $aSANTOS, L. S. dos 700 1 $aMICHELI, F. 700 1 $aMARGIS, R. 700 1 $aGESTEIRA, A. da S. 700 1 $aALVIM, F. C. 700 1 $aPEREIRA, G. A. G. 700 1 $aCASCARDO, J. C. de M. 773 $tPlanta, Heidelberg$gv. 232, n. 6, p. 1485–1497, 2010.
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