|
|
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Semiárido. |
Data corrente: |
28/08/2012 |
Data da última atualização: |
07/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FRANÇA, C. A.; PEIXOTO, R. M.; CAVALCANTE, M. B.; MELO, N. F. de; OLIVEIRA, C. J. B.; VESCHI, J. L. A.; MOTA, R. A.; COSTA, M. M. |
Afiliação: |
CHIRLES A. FRANÇA; RODOLFO M. PEIXOTO, Instituto Federal de Educação, Ciência e Tecnologia do Sertão Pernambucano, Campus Floresta; MARIELLY B. CAVALCANTE; NATONIEL FRANKLIN DE MELO, CPATSA; CELSO JOSÉ B. OLIVEIRA, UFPB; JOSIR LAINE APARECIDA VESCHI, CPATSA; RINALDO A. MOTA, UFRPE; MATEUS M. COSTA, UNIVASF. |
Título: |
Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, v. 32, n. 8, p. 747-753, ago. 2012. |
Idioma: |
Inglês Português |
Conteúdo: |
Abstract: The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efflux pump test was performed using ethidium bromide and biofilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-susceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efflux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isolates presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efflux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.
[Resistência antimicrobiana de Staphylococcus spp. de mastite de pequenos ruminantes no Brasil].
Resumo: O presente trabalho teve como objetivo determinar os padrões de resistência a agentes antimicrobianos e identificar marcadores moleculares de resistência em Staphylococcus spp. (n=210) isolados de mastite de pequenos ruminantes no Brasil. Os padrões de resistência a agentes antimicrobianos foram avaliados pelo teste de difusão em disco e pela detecção da presença dos genes mecA, blaZ, ermA, ermB, ermC e msrA via PCR. O teste da bomba de efluxo foi realizado utilizando brometo de etídio e a produção de biofime foi determinada pelo teste do vermelho congo em paralelo com o PCR para detecção do gene icaD. Os isolados foram mais resistentes a amoxicilina (50,0%), estreptomicina (42,8%), tetraciclina (40,4%), lincomicina (39,0%) e eritromicina (33,8%). Setenta e um (33,8%) isolados foram sensíveis a todas as drogas testadas e 41 foram positivos para a bomba de efluxo. Embora a resistência fenotípica a oxacilina tenha sido observada observada em 12,8% dos isolados, nenhum possuiu o gene mecA. Entretanto, 45,7% dos isolados continham a gene blaZ, indicando que a produção de beta-lactamases foi o principal mecanismo associado com a resistência dos Staphylococcus aos beta-lactâmicos. Os outros determinantes de resistência a agentes antimicrobianos ermA, ermb, ermC e msrA foram observados em 1,4%, 10,4%, 16,2% e 0,9% dos isolados respectivamente. Além disso, o gene icaD foi detectado em 32,9% dos isolados. Setenta e três isolados (54 de cabras e 19 de ovelhas) foram negativos para todos os genes de resistência testados e 69 isolados apresentaram dois ou mais genes de resistência. A associação entre blaZ, ermA, ermB, ermC e bomba de efluxo foi observada em 17 isolados dos quais 14 eram oriundos de cabras e três de ovelhas. Os dados obtidos no presente estudo indicam a resistência dos isolados aos beta-lactâmicos, o que pode estar associado ao uso sem controle veterinário destas drogas nos animais. MenosAbstract: The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efflux pump test was performed using ethidium bromide and biofilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-susceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efflux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isolates presented two or more resi... Mostrar Tudo |
Palavras-Chave: |
Bomba de efluxo; Mastite; Microorganismo; Staphylococcus spp. |
Thesagro: |
Biofilme; Cabra; Caprino; Leite; Marcador molecular; Ovino; Resistência. |
Thesaurus Nal: |
Goats; Sheep; Staphylococcus. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/65150/1/Natoniel.pdf
|
Marc: |
LEADER 04890naa a2200373 a 4500 001 1932471 005 2023-06-07 008 2012 bl uuuu u00u1 u #d 100 1 $aFRANÇA, C. A. 245 $aAntimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil.$h[electronic resource] 260 $c2012 520 $aAbstract: The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efflux pump test was performed using ethidium bromide and biofilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-susceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efflux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isolates presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efflux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control. [Resistência antimicrobiana de Staphylococcus spp. de mastite de pequenos ruminantes no Brasil]. Resumo: O presente trabalho teve como objetivo determinar os padrões de resistência a agentes antimicrobianos e identificar marcadores moleculares de resistência em Staphylococcus spp. (n=210) isolados de mastite de pequenos ruminantes no Brasil. Os padrões de resistência a agentes antimicrobianos foram avaliados pelo teste de difusão em disco e pela detecção da presença dos genes mecA, blaZ, ermA, ermB, ermC e msrA via PCR. O teste da bomba de efluxo foi realizado utilizando brometo de etídio e a produção de biofime foi determinada pelo teste do vermelho congo em paralelo com o PCR para detecção do gene icaD. Os isolados foram mais resistentes a amoxicilina (50,0%), estreptomicina (42,8%), tetraciclina (40,4%), lincomicina (39,0%) e eritromicina (33,8%). Setenta e um (33,8%) isolados foram sensíveis a todas as drogas testadas e 41 foram positivos para a bomba de efluxo. Embora a resistência fenotípica a oxacilina tenha sido observada observada em 12,8% dos isolados, nenhum possuiu o gene mecA. Entretanto, 45,7% dos isolados continham a gene blaZ, indicando que a produção de beta-lactamases foi o principal mecanismo associado com a resistência dos Staphylococcus aos beta-lactâmicos. Os outros determinantes de resistência a agentes antimicrobianos ermA, ermb, ermC e msrA foram observados em 1,4%, 10,4%, 16,2% e 0,9% dos isolados respectivamente. Além disso, o gene icaD foi detectado em 32,9% dos isolados. Setenta e três isolados (54 de cabras e 19 de ovelhas) foram negativos para todos os genes de resistência testados e 69 isolados apresentaram dois ou mais genes de resistência. A associação entre blaZ, ermA, ermB, ermC e bomba de efluxo foi observada em 17 isolados dos quais 14 eram oriundos de cabras e três de ovelhas. Os dados obtidos no presente estudo indicam a resistência dos isolados aos beta-lactâmicos, o que pode estar associado ao uso sem controle veterinário destas drogas nos animais. 650 $aGoats 650 $aSheep 650 $aStaphylococcus 650 $aBiofilme 650 $aCabra 650 $aCaprino 650 $aLeite 650 $aMarcador molecular 650 $aOvino 650 $aResistência 653 $aBomba de efluxo 653 $aMastite 653 $aMicroorganismo 653 $aStaphylococcus spp 700 1 $aPEIXOTO, R. M. 700 1 $aCAVALCANTE, M. B. 700 1 $aMELO, N. F. de 700 1 $aOLIVEIRA, C. J. B. 700 1 $aVESCHI, J. L. A. 700 1 $aMOTA, R. A. 700 1 $aCOSTA, M. M. 773 $tPesquisa Veterinária Brasileira$gv. 32, n. 8, p. 747-753, ago. 2012.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Semiárido (CPATSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
25/09/2018 |
Data da última atualização: |
11/03/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ATTIAS, N.; OLIVEIRA-SANTOS, L. G. R.; FAGAN, W. B.; MOURAO, G. de M. |
Afiliação: |
NINA ATTIAS, UFMS; LUIZ GUSTAVO RODRIGUES OLIVEIRA-SANTOS, UFMS; WILLIAM B. FAGAN, UNIVERSITY OF MARYLAND, U. S. A.; GUILHERME DE MIRANDA MOURAO, CPAP. |
Título: |
Effects of air temperature on habitat selection and activity patterns of two tropical imperfect homeotherms. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Animal Behaviour, v. 140, p. 129-140, 2018. |
DOI: |
https://doi.org/10.1016/j.anbehav.2018.04.011 |
Idioma: |
Inglês |
Notas: |
Na publicação: Guilherme Mourão. |
Conteúdo: |
In this study, we aimed to evaluate how air temperature is related to variation in activity patterns and habitat selection by two species of tropical armadillos, which are imperfect homeotherms. Although their behaviour is little studied, armadillos provide valuable models for understanding how physiology affects mammalian behaviour in response to environmental changes. We used GPS devices to track yellow armadillos, Euphractus sexcinctus, and southern three-banded armadillos, Tolypeutes matacus, at three sites of the Pantanal wetlands, Brazil. We used linear mixed-effects models to evaluate the variation in the timing and duration of activity patterns according to changes in air temperature. We fitted step selection functions to evaluate the effects of cover type, diel cycle and air temperature on armadillo habitat selection. Our models suggest that E. sexcinctus activity during the daytime decreases as air temperature increases. In contrast, T. matacus shows less variation, maintaining a predominantly nocturnal activity pattern. However, as air temperature decreases, activity periods of T. matacus are of shorter duration and peak earlier in the day. Both species should select forested areas when experiencing air temperatures outside their thermoneutral zones, as these areas act as thermal shelters. This study provides specific examples of the dynamic nature of activity patterns and habitat selection, and illustrates how thermal constraints, which vary dynamically over the daily cycle and among days, can alter behaviour. Our results highlight the importance of habitat heterogeneity for the long-term conservation of animal species that rely on behaviour to achieve adequate thermoregulation. MenosIn this study, we aimed to evaluate how air temperature is related to variation in activity patterns and habitat selection by two species of tropical armadillos, which are imperfect homeotherms. Although their behaviour is little studied, armadillos provide valuable models for understanding how physiology affects mammalian behaviour in response to environmental changes. We used GPS devices to track yellow armadillos, Euphractus sexcinctus, and southern three-banded armadillos, Tolypeutes matacus, at three sites of the Pantanal wetlands, Brazil. We used linear mixed-effects models to evaluate the variation in the timing and duration of activity patterns according to changes in air temperature. We fitted step selection functions to evaluate the effects of cover type, diel cycle and air temperature on armadillo habitat selection. Our models suggest that E. sexcinctus activity during the daytime decreases as air temperature increases. In contrast, T. matacus shows less variation, maintaining a predominantly nocturnal activity pattern. However, as air temperature decreases, activity periods of T. matacus are of shorter duration and peak earlier in the day. Both species should select forested areas when experiencing air temperatures outside their thermoneutral zones, as these areas act as thermal shelters. This study provides specific examples of the dynamic nature of activity patterns and habitat selection, and illustrates how thermal constraints, which vary dynamically over the ... Mostrar Tudo |
Thesagro: |
Comportamento Animal; Conservação; Ecologia Animal; Tatu. |
Thesaurus NAL: |
Animal behavior; Animal ecology; Dasypodidae; Telemetry. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02574naa a2200277 a 4500 001 2096243 005 2019-03-11 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.anbehav.2018.04.011$2DOI 100 1 $aATTIAS, N. 245 $aEffects of air temperature on habitat selection and activity patterns of two tropical imperfect homeotherms.$h[electronic resource] 260 $c2018 500 $aNa publicação: Guilherme Mourão. 520 $aIn this study, we aimed to evaluate how air temperature is related to variation in activity patterns and habitat selection by two species of tropical armadillos, which are imperfect homeotherms. Although their behaviour is little studied, armadillos provide valuable models for understanding how physiology affects mammalian behaviour in response to environmental changes. We used GPS devices to track yellow armadillos, Euphractus sexcinctus, and southern three-banded armadillos, Tolypeutes matacus, at three sites of the Pantanal wetlands, Brazil. We used linear mixed-effects models to evaluate the variation in the timing and duration of activity patterns according to changes in air temperature. We fitted step selection functions to evaluate the effects of cover type, diel cycle and air temperature on armadillo habitat selection. Our models suggest that E. sexcinctus activity during the daytime decreases as air temperature increases. In contrast, T. matacus shows less variation, maintaining a predominantly nocturnal activity pattern. However, as air temperature decreases, activity periods of T. matacus are of shorter duration and peak earlier in the day. Both species should select forested areas when experiencing air temperatures outside their thermoneutral zones, as these areas act as thermal shelters. This study provides specific examples of the dynamic nature of activity patterns and habitat selection, and illustrates how thermal constraints, which vary dynamically over the daily cycle and among days, can alter behaviour. Our results highlight the importance of habitat heterogeneity for the long-term conservation of animal species that rely on behaviour to achieve adequate thermoregulation. 650 $aAnimal behavior 650 $aAnimal ecology 650 $aDasypodidae 650 $aTelemetry 650 $aComportamento Animal 650 $aConservação 650 $aEcologia Animal 650 $aTatu 700 1 $aOLIVEIRA-SANTOS, L. G. R. 700 1 $aFAGAN, W. B. 700 1 $aMOURAO, G. de M. 773 $tAnimal Behaviour$gv. 140, p. 129-140, 2018.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pantanal (CPAP) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|