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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
06/09/2022 |
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Data da última atualização: |
08/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GAVA, C. A. T.; CASTRO, A. P. C. de; PEREIRA, C. A.; GONÇALVES, J. S.; ARAÚJO, L. F. C.; PAZ, C. D. da. |
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Afiliação: |
CARLOS ALBERTO TUAO GAVA, CPATSA; ANA PAULA CARVALHO DE CASTRO, UNEB/DTCS; CARLIANA ARAÚJO PEREIRA, UNEB/DTCS; JOSÉLIA SANTANA GONÇALVES, UPE; LUDMILLA FERREIRA CAJUHI ARAÚJO, UPE; CRISTIANE DOMINGOS DA PAZ, UNEB/DTCS. |
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Título: |
Photoprotector adjuvants to enhance UV tolerance of yeast strains for controlling mango decay using pre-harvest spraying. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biocontrol Science and Technology, v. 28, n. 9, p. 811-822, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Post-harvest mango decay is caused by multiple pathogens in tropical conditions but concerns regarding the risk of food contamination by fungicides established biocontrol as a promising alternative. However, occurrence of quiescent infections requires pre-harvest applications of biocontrol agents (BCA), exposing them to harmful UV radiation effects. The objective of this work was to evaluate UV sensitivity of yeast BCA strains previously selected against multiple pathogens that cause mango decay and evaluate suitable UV protectants. In a first bioassay conducted exposing yeast suspensions sprayed on glass plates, it was verified that Saccharomyces sp. ESA47 and Pichia kudriavzevii CMIAT171 were highly sensitive to UV, while Saccharomyces cerevisiae ESA45 had a slightly lower mortality. A bioassay using fragments of mango peels evaluated UV protection from increasing concentrations of starch, dextrin, casein, benzophenone, and cinnamic acid derivative compounds. Results showed that starch and isoamyl p-methoxycinnamate (NHE-1000) resulted in higher survival for yeast strains in doses of 10.0 and 1.0 g kg−1, respectively. Application of the yeast BCA in a semi-commercial mango orchard resulted in a significant reduction of post-harvest disease incidence and severity. Field application of the yeasts in technical grade preparations containing both UV protectants enhanced the control efficiency by 52.5, 31.9, and 37.7% for ESA45, ESA47, and CMIAT171, respectively. MenosPost-harvest mango decay is caused by multiple pathogens in tropical conditions but concerns regarding the risk of food contamination by fungicides established biocontrol as a promising alternative. However, occurrence of quiescent infections requires pre-harvest applications of biocontrol agents (BCA), exposing them to harmful UV radiation effects. The objective of this work was to evaluate UV sensitivity of yeast BCA strains previously selected against multiple pathogens that cause mango decay and evaluate suitable UV protectants. In a first bioassay conducted exposing yeast suspensions sprayed on glass plates, it was verified that Saccharomyces sp. ESA47 and Pichia kudriavzevii CMIAT171 were highly sensitive to UV, while Saccharomyces cerevisiae ESA45 had a slightly lower mortality. A bioassay using fragments of mango peels evaluated UV protection from increasing concentrations of starch, dextrin, casein, benzophenone, and cinnamic acid derivative compounds. Results showed that starch and isoamyl p-methoxycinnamate (NHE-1000) resulted in higher survival for yeast strains in doses of 10.0 and 1.0 g kg−1, respectively. Application of the yeast BCA in a semi-commercial mango orchard resulted in a significant reduction of post-harvest disease incidence and severity. Field application of the yeasts in technical grade preparations containing both UV protectants enhanced the control efficiency by 52.5, 31.9, and 37.7% for ESA45, ESA47, and CMIAT171, respectivel... Mostrar Tudo |
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Palavras-Chave: |
Podridão da manga; Protetores UV. |
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Thesagro: |
Controle Biológico; Doença; Manga; Mangifera Indica; Pós-Colheita. |
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Thesaurus Nal: |
Mangoes; Postharvest technology; Postharvest treatment. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146190/1/Photoprotector-adjuvants-to-enhance-UV-2018.pdf
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Marc: |
LEADER 02411naa a2200301 a 4500
001 2146190
005 2022-09-08
008 2018 bl uuuu u00u1 u #d
100 1 $aGAVA, C. A. T.
245 $aPhotoprotector adjuvants to enhance UV tolerance of yeast strains for controlling mango decay using pre-harvest spraying.$h[electronic resource]
260 $c2018
520 $aPost-harvest mango decay is caused by multiple pathogens in tropical conditions but concerns regarding the risk of food contamination by fungicides established biocontrol as a promising alternative. However, occurrence of quiescent infections requires pre-harvest applications of biocontrol agents (BCA), exposing them to harmful UV radiation effects. The objective of this work was to evaluate UV sensitivity of yeast BCA strains previously selected against multiple pathogens that cause mango decay and evaluate suitable UV protectants. In a first bioassay conducted exposing yeast suspensions sprayed on glass plates, it was verified that Saccharomyces sp. ESA47 and Pichia kudriavzevii CMIAT171 were highly sensitive to UV, while Saccharomyces cerevisiae ESA45 had a slightly lower mortality. A bioassay using fragments of mango peels evaluated UV protection from increasing concentrations of starch, dextrin, casein, benzophenone, and cinnamic acid derivative compounds. Results showed that starch and isoamyl p-methoxycinnamate (NHE-1000) resulted in higher survival for yeast strains in doses of 10.0 and 1.0 g kg−1, respectively. Application of the yeast BCA in a semi-commercial mango orchard resulted in a significant reduction of post-harvest disease incidence and severity. Field application of the yeasts in technical grade preparations containing both UV protectants enhanced the control efficiency by 52.5, 31.9, and 37.7% for ESA45, ESA47, and CMIAT171, respectively.
650 $aMangoes
650 $aPostharvest technology
650 $aPostharvest treatment
650 $aControle Biológico
650 $aDoença
650 $aManga
650 $aMangifera Indica
650 $aPós-Colheita
653 $aPodridão da manga
653 $aProtetores UV
700 1 $aCASTRO, A. P. C. de
700 1 $aPEREIRA, C. A.
700 1 $aGONÇALVES, J. S.
700 1 $aARAÚJO, L. F. C.
700 1 $aPAZ, C. D. da
773 $tBiocontrol Science and Technology$gv. 28, n. 9, p. 811-822, 2018.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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Data corrente: |
16/03/2020 |
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Data da última atualização: |
20/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
HAEHLING, M. B. V.; CRUVINEL, G. G.; TOSCANO, J. H. B.; GIRALDELO, L. A.; SANTOS, I. B. dos; ESTEVES, S. N.; BENAVIDES, M. V.; BARIONI JUNIOR, W.; NICIURA, S. C. M.; CHAGAS, A. C. de S. |
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Afiliação: |
MAREI BORSCH VON HAEHLING, UNESP; GIOVANNA GABRIELLE CRUVINEL, UNICEP; JOÃO HENRIQUE BARBOSA TOSCANO, UNESP; LUCIANA APARECIDA GIRALDELO, UNICEP; ISABELLA BARBOSA DOS SANTOS, UNESP; SERGIO NOVITA ESTEVES, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; WALDOMIRO BARIONI JUNIOR, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Four single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 279, 2020, 109053. |
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Páginas: |
11 p. |
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ISSN: |
0304-4017 |
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DOI: |
https://doi.org/10.1016/j.vetpar.2020.109053 |
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Idioma: |
Inglês |
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Conteúdo: |
Gastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep. MenosGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an... Mostrar Tudo |
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Palavras-Chave: |
Host resistance; Morada Nova sheep; Parasite challenge; SNPs. |
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Thesaurus NAL: |
Genetic markers. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03156naa a2200325 a 4500
001 2121271
005 2020-04-20
008 2020 bl uuuu u00u1 u #d
022 $a0304-4017
024 7 $ahttps://doi.org/10.1016/j.vetpar.2020.109053$2DOI
100 1 $aHAEHLING, M. B. V.
245 $aFour single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep.$h[electronic resource]
260 $c2020
300 $a11 p.
520 $aGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep.
650 $aGenetic markers
653 $aHost resistance
653 $aMorada Nova sheep
653 $aParasite challenge
653 $aSNPs
700 1 $aCRUVINEL, G. G.
700 1 $aTOSCANO, J. H. B.
700 1 $aGIRALDELO, L. A.
700 1 $aSANTOS, I. B. dos
700 1 $aESTEVES, S. N.
700 1 $aBENAVIDES, M. V.
700 1 $aBARIONI JUNIOR, W.
700 1 $aNICIURA, S. C. M.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv. 279, 2020, 109053.
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