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Registros recuperados : 41 | |
12. | | ROCHA, C. R. M. da; CRUZ, E. D.; MARTINS, L. de L.; SANTOS, C. S. dos. Caracterização de frutos oriundos de clones de cupuaçu. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. 1 CD-ROM. Biblioteca(s): Embrapa Amazônia Oriental. |
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14. | | RIBEIRO, M. E. R.; KOLLING, G. J.; ZANELA, M. B.; SANTOS, C. S. dos; PERIN, L.; FISCHER, V. Composição do leite normal e do LINA em rebanho jersey: resultados preliminares 2011. In.: CONFERENCIA INTERNACIONAL SOBRE LECHE INESTABLE, 2., 2011, Colonia. [Anais...]. Colonia: Instituto Nacional de Investigación Agropecuaria de Uruguay, 2011. p. 62. Biblioteca(s): Embrapa Clima Temperado. |
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16. | | SANTOS, C. S. dos; JESUS, V. L. T. de; McINTOSH, D.; BERTO, B. P.; LOPES, C. W. G. Co-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 12, p. 980-988, dez. 2015. Biblioteca(s): Embrapa Unidades Centrais. |
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17. | | SANTOS, C. S. dos; MATOS, N. M. S. de; REZENDE, T. T.; MAURI, J.; RODRIGUES, G. C.; VEIGA, A. D.; BARTHOLO, G. F.; CARVALHO, M. A. de F. Agronomic, anatomic and physiological characterization of Coffea arabica L. genotypes on irrigated system in the Central Cerrado. Coffee Science, v. 17, e172021, 2022. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Café; Embrapa Cerrados. |
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18. | | SANTOS, C. S. dos; PEREIRA, F. A. C.; CARVALHO, M. A. de F.; MENDES, A. N. G.; RODRIGUES, G. C.; VEIGA, A. D. Diversidade genética de genótipos de Coffea arabica em condições de Cerrado do Planalto Central do Brasil. In: INTERNATIONAL SYMPOSIUM IN GENETICS AND PLANT BREEDING, 22., 2018, Lavras. Anais... Lavras: Ed. UFLA, 2018. p. 59. Biblioteca(s): Embrapa Agricultura Digital. |
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19. | | SANTOS, C. S. dos; PEREIRA, F. A. C.; CARVALHO, M. A. de F.; MENDES, A. N. G.; RODRIGUES, G. C.; VEIGA, A. D. Divergência genética com base em características anatômicas foliares de genótipos de Coffea arabica L. In: INTERNATIONAL SYMPOSIUM IN GENETICS AND PLANT BREEDING, 23., 2019, Lavras. Anais... Lavras: UFLA, 2019. p. 25. Biblioteca(s): Embrapa Agricultura Digital. |
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20. | | SANTOS, C. S. dos; PEREIRA, F. A. C.; CARVALHO, M. A. de F.; MENDES, A. N. G.; RODRIGUES, G. C.; VEIGA, A. D. Divergência genética com base em características anatômicas foliares de genótipos de Coffea arabica L. In: INTERNATIONAL SYMPOSIUM IN GENETICS AND PLANT BREEDING, 23., 2019, Lavras. Anais... Lavras: UFLA, 2019. p. 25. Biblioteca(s): Embrapa Café. |
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Registros recuperados : 41 | |
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Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
25/04/2016 |
Data da última atualização: |
25/04/2016 |
Autoria: |
SANTOS, C. S. dos; JESUS, V. L. T. de; McINTOSH, D.; BERTO, B. P.; LOPES, C. W. G. |
Afiliação: |
CAROLINE SPITZ DOS SANTOS, UFRRJ; VERA LÚCIA TEIXEIRA DE JESUS, UFRRJ; DOUGLAS McINTOSH, UFRRJ; BRUNO PEREIRA BERTO, UFRRJ; CARLOS WILSON GOMES LOPES, UFRRJ. |
Título: |
Co-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 12, p. 980-988, dez. 2015. |
Idioma: |
Inglês |
Conteúdo: |
Tritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle endingabruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of the four samples (3.89%) sequencing revealed 100% similarity when compared with another sequence of P. hominis deposited in Genbank (KC623939). Therefore, the present study revealed through the diagnostic techniques employed the simultaneous infection by T. foetus and P. hominis in the feces of cats. However, it was necessary to use more than one technique for the diagnosis of the co-infection. These results demonstrate the importance of a correct diagnosis to allow an appropriate treatment by the veterinarian. MenosTritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle endingabruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of t... Mostrar Tudo |
Palavras-Chave: |
Domestic cats; Gato doméstico. |
Thesagro: |
Tritrichomonas Foetus. |
Thesaurus NAL: |
Mixed infection; Pentatrichomonas hominis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142564/1/Co-infection-by-tritrichnomonas.pdf
|
Marc: |
LEADER 02713naa a2200229 a 4500 001 2043968 005 2016-04-25 008 2015 bl uuuu u00u1 u #d 100 1 $aSANTOS, C. S. dos 245 $aCo-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats. 260 $c2015 520 $aTritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle endingabruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of the four samples (3.89%) sequencing revealed 100% similarity when compared with another sequence of P. hominis deposited in Genbank (KC623939). Therefore, the present study revealed through the diagnostic techniques employed the simultaneous infection by T. foetus and P. hominis in the feces of cats. However, it was necessary to use more than one technique for the diagnosis of the co-infection. These results demonstrate the importance of a correct diagnosis to allow an appropriate treatment by the veterinarian. 650 $aMixed infection 650 $aPentatrichomonas hominis 650 $aTritrichomonas Foetus 653 $aDomestic cats 653 $aGato doméstico 700 1 $aJESUS, V. L. T. de 700 1 $aMcINTOSH, D. 700 1 $aBERTO, B. P. 700 1 $aLOPES, C. W. G. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 12, p. 980-988, dez. 2015.
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