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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
03/04/2013 |
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Data da última atualização: |
04/07/2016 |
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Autoria: |
CARVALHO, V. S.; ARAÚJO, B. R.; VASCONCELOS, T. C.; CHIMINAZZO, C.; COSTA NETO, A. O.; AYRES, M. C. C.; GUIMARÃES, J. E.; COSTA, J. N. |
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Título: |
Evolução clínica e avaliação de parâmetros leucocitários e de proteínas de fase aguda na pododermatite infecciosa ovina. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 32, n. 12, p. 1289-1296, dez., 2012. |
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DOI: |
http://dx.doi.org/10.1590/S0100-736X2012001200013 |
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Idioma: |
Português |
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Conteúdo: |
Resumo: Neste artigo realizou-se a avaliação de ovinos mestiços Santa Inês com pododermatite infecciosa, verificando as alterações no leucograma e proteínas de fase aguda. No primeiro experimento, 70 fêmeas foram separadas em três grupos de acordo com o seu escore podal: Grupo controle (G1) com escore 0; Dermatite interdigital (G2) com escore 1 ou 2 e Pododermatite necrosante (G3) com escore 3, 4 ou 5. Durante dois meses observou-se a evolução clínica e efeitos no leucograma em cinco momentos: M1 (dia 0), M2 (dia 15), M3 (dia 30), M4 (dia 45) e M5 (dia 60). A intensidade do resultado do leucograma foi de baixa magnitude, observando-se alterações significativas (p<0,05) como uma leve leucocitose (G3, M4), atribuída a neutrofilia e um discreto aumento no número total de monócitos (G3, M2 e M3) apesar do extenso dano e necrose tecidual existentes na última fase da doença. No segundo experimento, utilizaram-se 105 animais de sete propriedades com objetivo de isolar o agente etiológico e avaliar os efeitos da doença sobre proteína plasmática total e proteínas de fase aguda. Os animais também foram separados em três grupos: controle, dermatite interdigital e pododermatite necrosante. Em todas as propriedades foi realizado o isolamento de Dichelobacter nodosus. Não houve correlação significativa (p<0,05) das diferentes fases da doença sobre as proteínas estudadas, porém a haptoglobina dos grupos com animais doentes apresentou médias superiores ao grupo controle. De acordo com a metodologia utilizada e resultados obtidos, conclui-se que as proteínas de fase aguda estudadas não foram eficientes na caracterização das fases da pododermatite infecciosa ovina e que a resposta leucocitária foi branda, tornando difícil sua utilização para este fim.
[Clinical evolution and evaluation of leukocyte parameters and acute phase protein of ovine footrot].
Abstract: In this paper, Santa Inês crossbred sheep with footrot were evaluated, checking the changes on leukogram and the acute phase proteins. In the first experiment, 70 females were divided into three groups according to their podal scores: a control group (G1) with score 0, scald group (G2) with score 1 or 2 and a footrot group (G3) with score 3, 4 or 5. During two months, the clinical course and its effect on leukogram were observed at five moments: M1 (day 0), M2 (day 15), M3 (day 30), M4 (day 45) and M5 (day 60). The intensity of the white blood cell count was low magnitude, observing significant changes (p <0.05) as a mild leukocytosis (G3, M4), attributed to neutrophilia and a slight increase in the total number of monocytes (G3, M2 and M3) despite the extensive damage and tissue necrosis existed during the final stage of the disease. In the second experiment, 105 animals from seven farms were used with the objective of isolating the causative agent and to evaluate the effect of disease on total plasma protein and acute phase proteins. The animals were also divided into three groups: control, scald and footrot. In all farms, the isolation of Dichelobacter nodosus was regarded. For the different stages of the disease on the proteins studied there was no significant correlation (p<0.05) but the haptoglobin in scald and footrot groups showed higher average than the control group. Based on these results, we conclude that the acute phase proteins studied were not efficient in the characterization of the phases of the disease and the leukocyte response was mild, making it difficult to use for this purpose. MenosResumo: Neste artigo realizou-se a avaliação de ovinos mestiços Santa Inês com pododermatite infecciosa, verificando as alterações no leucograma e proteínas de fase aguda. No primeiro experimento, 70 fêmeas foram separadas em três grupos de acordo com o seu escore podal: Grupo controle (G1) com escore 0; Dermatite interdigital (G2) com escore 1 ou 2 e Pododermatite necrosante (G3) com escore 3, 4 ou 5. Durante dois meses observou-se a evolução clínica e efeitos no leucograma em cinco momentos: M1 (dia 0), M2 (dia 15), M3 (dia 30), M4 (dia 45) e M5 (dia 60). A intensidade do resultado do leucograma foi de baixa magnitude, observando-se alterações significativas (p<0,05) como uma leve leucocitose (G3, M4), atribuída a neutrofilia e um discreto aumento no número total de monócitos (G3, M2 e M3) apesar do extenso dano e necrose tecidual existentes na última fase da doença. No segundo experimento, utilizaram-se 105 animais de sete propriedades com objetivo de isolar o agente etiológico e avaliar os efeitos da doença sobre proteína plasmática total e proteínas de fase aguda. Os animais também foram separados em três grupos: controle, dermatite interdigital e pododermatite necrosante. Em todas as propriedades foi realizado o isolamento de Dichelobacter nodosus. Não houve correlação significativa (p<0,05) das diferentes fases da doença sobre as proteínas estudadas, porém a haptoglobina dos grupos com animais doentes apresentou médias superiores ao grupo controle. De acordo com a met... Mostrar Tudo |
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Palavras-Chave: |
Haptoglobina; Pododermatite. |
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Thesagro: |
Bactéria; Doença animal; Leucograma; Ovino. |
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Thesaurus Nal: |
Dichelobacter nodosus; Fusobacterium necrophorum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04562naa a2200313 a 4500
001 1954866
005 2016-07-04
008 2012 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/S0100-736X2012001200013$2DOI
100 1 $aCARVALHO, V. S.
245 $aEvolução clínica e avaliação de parâmetros leucocitários e de proteínas de fase aguda na pododermatite infecciosa ovina.$h[electronic resource]
260 $c2012
520 $aResumo: Neste artigo realizou-se a avaliação de ovinos mestiços Santa Inês com pododermatite infecciosa, verificando as alterações no leucograma e proteínas de fase aguda. No primeiro experimento, 70 fêmeas foram separadas em três grupos de acordo com o seu escore podal: Grupo controle (G1) com escore 0; Dermatite interdigital (G2) com escore 1 ou 2 e Pododermatite necrosante (G3) com escore 3, 4 ou 5. Durante dois meses observou-se a evolução clínica e efeitos no leucograma em cinco momentos: M1 (dia 0), M2 (dia 15), M3 (dia 30), M4 (dia 45) e M5 (dia 60). A intensidade do resultado do leucograma foi de baixa magnitude, observando-se alterações significativas (p<0,05) como uma leve leucocitose (G3, M4), atribuída a neutrofilia e um discreto aumento no número total de monócitos (G3, M2 e M3) apesar do extenso dano e necrose tecidual existentes na última fase da doença. No segundo experimento, utilizaram-se 105 animais de sete propriedades com objetivo de isolar o agente etiológico e avaliar os efeitos da doença sobre proteína plasmática total e proteínas de fase aguda. Os animais também foram separados em três grupos: controle, dermatite interdigital e pododermatite necrosante. Em todas as propriedades foi realizado o isolamento de Dichelobacter nodosus. Não houve correlação significativa (p<0,05) das diferentes fases da doença sobre as proteínas estudadas, porém a haptoglobina dos grupos com animais doentes apresentou médias superiores ao grupo controle. De acordo com a metodologia utilizada e resultados obtidos, conclui-se que as proteínas de fase aguda estudadas não foram eficientes na caracterização das fases da pododermatite infecciosa ovina e que a resposta leucocitária foi branda, tornando difícil sua utilização para este fim. [Clinical evolution and evaluation of leukocyte parameters and acute phase protein of ovine footrot]. Abstract: In this paper, Santa Inês crossbred sheep with footrot were evaluated, checking the changes on leukogram and the acute phase proteins. In the first experiment, 70 females were divided into three groups according to their podal scores: a control group (G1) with score 0, scald group (G2) with score 1 or 2 and a footrot group (G3) with score 3, 4 or 5. During two months, the clinical course and its effect on leukogram were observed at five moments: M1 (day 0), M2 (day 15), M3 (day 30), M4 (day 45) and M5 (day 60). The intensity of the white blood cell count was low magnitude, observing significant changes (p <0.05) as a mild leukocytosis (G3, M4), attributed to neutrophilia and a slight increase in the total number of monocytes (G3, M2 and M3) despite the extensive damage and tissue necrosis existed during the final stage of the disease. In the second experiment, 105 animals from seven farms were used with the objective of isolating the causative agent and to evaluate the effect of disease on total plasma protein and acute phase proteins. The animals were also divided into three groups: control, scald and footrot. In all farms, the isolation of Dichelobacter nodosus was regarded. For the different stages of the disease on the proteins studied there was no significant correlation (p<0.05) but the haptoglobin in scald and footrot groups showed higher average than the control group. Based on these results, we conclude that the acute phase proteins studied were not efficient in the characterization of the phases of the disease and the leukocyte response was mild, making it difficult to use for this purpose.
650 $aDichelobacter nodosus
650 $aFusobacterium necrophorum
650 $aBactéria
650 $aDoença animal
650 $aLeucograma
650 $aOvino
653 $aHaptoglobina
653 $aPododermatite
700 1 $aARAÚJO, B. R.
700 1 $aVASCONCELOS, T. C.
700 1 $aCHIMINAZZO, C.
700 1 $aCOSTA NETO, A. O.
700 1 $aAYRES, M. C. C.
700 1 $aGUIMARÃES, J. E.
700 1 $aCOSTA, J. N.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 32, n. 12, p. 1289-1296, dez., 2012.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
25/04/2016 |
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Data da última atualização: |
25/04/2016 |
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Autoria: |
SANTOS, C. S. dos; JESUS, V. L. T. de; McINTOSH, D.; BERTO, B. P.; LOPES, C. W. G. |
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Afiliação: |
CAROLINE SPITZ DOS SANTOS, UFRRJ; VERA LÚCIA TEIXEIRA DE JESUS, UFRRJ; DOUGLAS McINTOSH, UFRRJ; BRUNO PEREIRA BERTO, UFRRJ; CARLOS WILSON GOMES LOPES, UFRRJ. |
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Título: |
Co-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 12, p. 980-988, dez. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Tritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle endingabruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of the four samples (3.89%) sequencing revealed 100% similarity when compared with another sequence of P. hominis deposited in Genbank (KC623939). Therefore, the present study revealed through the diagnostic techniques employed the simultaneous infection by T. foetus and P. hominis in the feces of cats. However, it was necessary to use more than one technique for the diagnosis of the co-infection. These results demonstrate the importance of a correct diagnosis to allow an appropriate treatment by the veterinarian. MenosTritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle endingabruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of t... Mostrar Tudo |
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Palavras-Chave: |
Domestic cats; Gato doméstico. |
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Thesagro: |
Tritrichomonas Foetus. |
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Thesaurus NAL: |
Mixed infection; Pentatrichomonas hominis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142564/1/Co-infection-by-tritrichnomonas.pdf
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Marc: |
LEADER 02713naa a2200229 a 4500
001 2043968
005 2016-04-25
008 2015 bl uuuu u00u1 u #d
100 1 $aSANTOS, C. S. dos
245 $aCo-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats.
260 $c2015
520 $aTritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle endingabruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of the four samples (3.89%) sequencing revealed 100% similarity when compared with another sequence of P. hominis deposited in Genbank (KC623939). Therefore, the present study revealed through the diagnostic techniques employed the simultaneous infection by T. foetus and P. hominis in the feces of cats. However, it was necessary to use more than one technique for the diagnosis of the co-infection. These results demonstrate the importance of a correct diagnosis to allow an appropriate treatment by the veterinarian.
650 $aMixed infection
650 $aPentatrichomonas hominis
650 $aTritrichomonas Foetus
653 $aDomestic cats
653 $aGato doméstico
700 1 $aJESUS, V. L. T. de
700 1 $aMcINTOSH, D.
700 1 $aBERTO, B. P.
700 1 $aLOPES, C. W. G.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 12, p. 980-988, dez. 2015.
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