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| Registros recuperados : 30 | |
| 6. |  | PEREIRA, T.; ALVAREZ, J. M.; SSANTOS, S. A.; AGUUILAR, M. R.; DESBIEZ, A.; GARCIA, J. B. Morfometria dos estômatos de quatro espécies de gramíneas temperadas no Pantanal. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PANTANAL, 1.; SEMANA DE BIOLOGIA, 7., 2007, Corumbá. Resumos...Corumbá: Embrapa Pantanal, 2007. (Embrapa Pantanal. Documentos, 89). 1 p.| Biblioteca(s): Embrapa Pantanal. |
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| 11. | | SANABRIA, L.; LAGRAVE, L.; NISHIBE, C.; RIBAS, A. C. A.; ZUMÁRRAGA, M. J.; ALMEIDA, N. F.; ARAUJO, F. R. Draft Genome Sequences of Two Mycobacterium bovis Strains Isolated from Beef Cattle in Paraguay. Genome Announcements, v. 5, n. 28, p. 16-17, jul. 2017.| Biblioteca(s): Embrapa Gado de Corte. |
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| 17. | | COSTA, F. H. da S.; PASQUAL, M.; SANTOS, A. M. dos; CASTRO, E. M. de; PEREIRA, J. E. S.; PIO, L. A.; COSTA, L. C. do. Relação entre o conteúdo relativo de água, densidade estomática e formação de cera epicuticular em folhas de plantas micropropagadas de bananeira. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 16.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 3.; SIMPÓSIO DE PLANTAS ORNAMENTAIS NATIVAS, 1., 2007, Goiânia. Biodiversidade e competitividade: buscando novas opções: anais. Campinas: Sociedade Brasileira de Floricultura e Plantas Ornamentais, 2007. p. 1145-1148.| Biblioteca(s): Embrapa Acre. |
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| Registros recuperados : 30 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
18/10/2011 |
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Data da última atualização: |
12/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MARTINS, A. C. Q.; MORGANTE, C. V.; SANTOS, C. M. R.; SILVA, F. R. da; ARAÚJO, A. C. G.; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M.; BRASILEIRO, A. C. M. |
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Afiliação: |
UnB; CAROLINA VIANNA MORGANTE, CPATSA; UnB; UnB; UnB; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
A transcriptional approach for identifying genes related to drought stress. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. |
|
Páginas: |
p. 77. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Stress-responsive mechanisms triggered in plants under drought stress affect plant growth and cause serious limitation to crop productivity. Due to its high genetic diversity and adaptation to a range of environments, wild relatives of peanut constitute a rich source of allele diversity for resistance to biotic and abiotic stresses. Based on previous data and as a 6rst step to identify drought-responsive genes inArachis, the wild specieA. magna, accession KG30097, was selected as it showed high adaptability to water stress conditions. The transcriptome of A. magna leaves subrnitted to gradual water stress was analyzed. Subtractive libraries were constructed with cDNA from leaf tissues of stressed and well-watered control plants. Subtractive hybridization was performed in both directions: cD A from stressed plants was used as driver and afterwards as tester, allowing for the enrichment of genes either induced or inhibited during water stress. In silicoanalysis revealed 759 reads, which were grouped into 249 clusters, with a novelty index of 32,8%. Several up and downregulated genes were identi6ed exclusively in stressed or control conditions. The expression proíile of some differentially regulated genes was validated by real time PCR, using cDNA Erom roots and leafs of stressed and control plants. Glycine descarboxilase, metallothionein-like protein, drought stress responsive protein, and two unknown proteins were shown to be up-regulated and the gene coding for a disease responsive protein was down-regulated. The information produced in this study is a valuable resource for gene identi6cation, characterization of new wild alleles, and development of molecular markers. MenosStress-responsive mechanisms triggered in plants under drought stress affect plant growth and cause serious limitation to crop productivity. Due to its high genetic diversity and adaptation to a range of environments, wild relatives of peanut constitute a rich source of allele diversity for resistance to biotic and abiotic stresses. Based on previous data and as a 6rst step to identify drought-responsive genes inArachis, the wild specieA. magna, accession KG30097, was selected as it showed high adaptability to water stress conditions. The transcriptome of A. magna leaves subrnitted to gradual water stress was analyzed. Subtractive libraries were constructed with cDNA from leaf tissues of stressed and well-watered control plants. Subtractive hybridization was performed in both directions: cD A from stressed plants was used as driver and afterwards as tester, allowing for the enrichment of genes either induced or inhibited during water stress. In silicoanalysis revealed 759 reads, which were grouped into 249 clusters, with a novelty index of 32,8%. Several up and downregulated genes were identi6ed exclusively in stressed or control conditions. The expression proíile of some differentially regulated genes was validated by real time PCR, using cDNA Erom roots and leafs of stressed and control plants. Glycine descarboxilase, metallothionein-like protein, drought stress responsive protein, and two unknown proteins were shown to be up-regulated and the gene coding for a disease res... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Estresse hídrico. |
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Thesagro: |
Amendoim; Marcador Molecular; Planta. |
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Thesaurus NAL: |
Peanuts. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/131239/1/ID-45694.pdf
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Marc: |
LEADER 02684nam a2200289 a 4500
001 1903303
005 2023-06-12
008 2011 bl uuuu u00u1 u #d
100 1 $aMARTINS, A. C. Q.
245 $aA transcriptional approach for identifying genes related to drought stress.
260 $aIn: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology$c2011
300 $ap. 77.
520 $aStress-responsive mechanisms triggered in plants under drought stress affect plant growth and cause serious limitation to crop productivity. Due to its high genetic diversity and adaptation to a range of environments, wild relatives of peanut constitute a rich source of allele diversity for resistance to biotic and abiotic stresses. Based on previous data and as a 6rst step to identify drought-responsive genes inArachis, the wild specieA. magna, accession KG30097, was selected as it showed high adaptability to water stress conditions. The transcriptome of A. magna leaves subrnitted to gradual water stress was analyzed. Subtractive libraries were constructed with cDNA from leaf tissues of stressed and well-watered control plants. Subtractive hybridization was performed in both directions: cD A from stressed plants was used as driver and afterwards as tester, allowing for the enrichment of genes either induced or inhibited during water stress. In silicoanalysis revealed 759 reads, which were grouped into 249 clusters, with a novelty index of 32,8%. Several up and downregulated genes were identi6ed exclusively in stressed or control conditions. The expression proíile of some differentially regulated genes was validated by real time PCR, using cDNA Erom roots and leafs of stressed and control plants. Glycine descarboxilase, metallothionein-like protein, drought stress responsive protein, and two unknown proteins were shown to be up-regulated and the gene coding for a disease responsive protein was down-regulated. The information produced in this study is a valuable resource for gene identi6cation, characterization of new wild alleles, and development of molecular markers.
650 $aPeanuts
650 $aAmendoim
650 $aMarcador Molecular
650 $aPlanta
653 $aDiversidade genética
653 $aEstresse hídrico
700 1 $aMORGANTE, C. V.
700 1 $aSANTOS, C. M. R.
700 1 $aSILVA, F. R. da
700 1 $aARAÚJO, A. C. G.
700 1 $aBERTIOLI, D. J.
700 1 $aBERTIOLI, S. C. de M. L.
700 1 $aGUIMARAES, P. M.
700 1 $aBRASILEIRO, A. C. M.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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