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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
02/01/2017 |
Data da última atualização: |
20/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOREIRA, C. M.; ANDRADE, H. B. de; BERTOLUCCI, S. K. V.; LAMEIRA, O. A.; MOHAMMED, A.; PINTO, J. E. B. P. |
Afiliação: |
Carolina Mariane MOREIRA, UFLA; Helena Botelho de ANDRADE, UFLA; Suzan Kelly Vilela BERTOLUCCI, UFLA; OSMAR ALVES LAMEIRA, CPATU; Aliyu MOHAMMED, Abant ?zzet Baysal University; José Eduardo Brasil Pereira PINTO, UFLA. |
Título: |
Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Turkish Journal of Biology, v. 40, n. 6, p. 1227-1234, 2016. |
DOI: |
10.3906/biy-1601-56 |
Idioma: |
Inglês |
Conteúdo: |
The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6- trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%. |
Palavras-Chave: |
Regeneração in vitro. |
Thesagro: |
Curaua; Regeneração. |
Thesaurus Nal: |
thidiazuron. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/152619/1/Carolina-Moreira-Plantlet-regeneration-curaua-2016.pdf
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Marc: |
LEADER 02130naa a2200241 a 4500 001 2059742 005 2022-05-20 008 2016 bl uuuu u00u1 u #d 024 7 $a10.3906/biy-1601-56$2DOI 100 1 $aMOREIRA, C. M. 245 $aPlantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species.$h[electronic resource] 260 $c2016 520 $aThe present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6- trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%. 650 $athidiazuron 650 $aCuraua 650 $aRegeneração 653 $aRegeneração in vitro 700 1 $aANDRADE, H. B. de 700 1 $aBERTOLUCCI, S. K. V. 700 1 $aLAMEIRA, O. A. 700 1 $aMOHAMMED, A. 700 1 $aPINTO, J. E. B. P. 773 $tTurkish Journal of Biology$gv. 40, n. 6, p. 1227-1234, 2016.
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Embrapa Amazônia Oriental (CPATU) |
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Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
13/11/2023 |
Data da última atualização: |
13/11/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MARTINS, M. B.; SANTOS, A. F. dos; AUER, C. G.; BUHRER, C. de B. |
Afiliação: |
MIRÉLI BERGMANN MARTINS, UNIVERSIDADE FEDERAL DO PARANÁ; ÁLVARO FIGUEREDO DOS SANTOS, UNIVERSIDADE FEDERAL DO PARANÁ; CELSO GARCIA AUER, CNPF; CAROLINE DE BASTOS BUHRER, CNPF. |
Título: |
Isolamento, obtenção de culturas monospóricas e preservação de isolados de Colletotrichum de erva-mate. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 21., 2022, Colombo. Anais... Colombo: Embrapa Florestas, 2023. |
Páginas: |
p. 33. |
Série: |
(Embrapa Florestas. Documentos, 384). |
Idioma: |
Português |
Palavras-Chave: |
Coleção de fungos. |
Thesagro: |
Antracnose; Fungo; Ilex Paraguariensis. |
Thesaurus NAL: |
Colletotrichum. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1158245/1/EmbrapaFlorestas-2023-Documentos384-Res-33.pdf
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Marc: |
LEADER 00763nam a2200217 a 4500 001 2158245 005 2023-11-13 008 2023 bl uuuu u00u1 u #d 100 1 $aMARTINS, M. B. 245 $aIsolamento, obtenção de culturas monospóricas e preservação de isolados de Colletotrichum de erva-mate.$h[electronic resource] 260 $aIn: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 21., 2022, Colombo. Anais... Colombo: Embrapa Florestas$c2023 300 $ap. 33. 490 $a(Embrapa Florestas. Documentos, 384). 650 $aColletotrichum 650 $aAntracnose 650 $aFungo 650 $aIlex Paraguariensis 653 $aColeção de fungos 700 1 $aSANTOS, A. F. dos 700 1 $aAUER, C. G. 700 1 $aBUHRER, C. de B.
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Embrapa Florestas (CNPF) |
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