Data/hora: 07/06/2024 14:08:43

Biblioteca(s): | Embrapa Soja.
Data corrente: | 26/04/2005
Data da última atualização: | 27/02/2020
Autoria: | SANTOS, B.; MOSCARDI, F.
Afiliação: | CNPSO.
Título: | Improvements in the large scale production of the velvetbean caterpillar, Anticarsia gemmatalis, nucleopolyhedrovirus in the laboratory.
Ano de publicação: | 2003
Fonte/Imprenta: | In: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington, 2003.
Páginas: | p. 45.
Idioma: | Inglês
Conteúdo: | The NPV of A. gemmatalis (AgMNPV) is currently being used on over 1,600,000 hectares of soybean in Brazil. Although two private companies had previously attempted to produce the AgMNPV in the laboratory, the ceased production because of the high costs involved, specially those related to artificial diet ingredients (mainly agar and casein), rearing recipients and labor. Thus, large-scale field production of the AgMNPV has been the sole method employed presently, but it is highly dependable on host abundance, wich is affected each year by abiotic and biotic factors, resulting in variable virus yields each season. The objective of this work to improve the laboratory mass production and processing of the AgMNPV, so as to turn the final product cost competitive with available chemical insecticides to control the insect. Initial studies were conducted to substitute the agar and reduce the amount of casein previously utilized for A. gemmatalis rearing and AgMNPV production. The use of Carragena GP-911 compared to the "Invitrogen P.A." and "All Chemistry" agars and reduction of casein by %0% proved successful for virus production, resulting in 86.2 to 95.7% reduction of the diet cost. The modified diet did not alter the survival rate or the weight of caterpillars and pupae. Larvae inoculated as fourth instars with 950,000 OBs/ml of diet in cardboard boxes (30x30) cm and 9 cm high) (350 larvae/box), and maintained at 28o.C provided the highest AgMNPV yield. In this conditions, efficiency of virus production was over 75% (with canibalism being the major cause of yield loss). Laboratory production yielded an average of 58.8 hectare equivalent (HE)/kg of AgMNPV dead larvae, compared to 40 to 50 HE/kg of dead larvae collected under field conditions. Also, the former method resulted in a much lower level of contaminats. The mechanical extratction of the AgMNPV from dead caterpillars (through an adapted fruit juicer) yielded 92.8% of the virus against 75.6% obtained from the manual extraction. Combining the results, the cost of the final AgMNPV product after its production in the laboratory, processing, formulation, quality control, and packaging aws reduced enormously compared to previous procedures, turning the biological insecticide cost competitive with the chemical insecticides. The new laboratory procedures is being proposed to some of the companies producing and commercializing the AgMNPV.
Thesagro: | Baculovirus; Controle Biológico; Praga de Planta; Produção; Soja.
Thesaurus Nal: | Soybeans.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (24950-1) - UPC - RA - DD -  -  -  - DIS