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| Registros recuperados : 20 | |
| 4. |  | SANTANA, R. H.; ALMEIDA, J. D.; CARNEIRO, V. T.; ABDELNOOR, R. V.; BARROS, L. M. Identification of soybean organ-specific expressed genes. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 104.| Biblioteca(s): Embrapa Soja. |
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| 5. | | SANTANA, R. H.; ALMEIDA, J. D.; CARNEIRO, V. T.; ABDELNOOR, R. V.; BARROS, L. M. G. Identification of soybean organ-specific expressed genes. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 104.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | SANTANA, R. H.; VERAS, H. C. T.; ABDELNOOR, R. V.; BARROS, L. M. G. Análise experimental da expressão diferencial de um transcrito de soja identificado através de northern eletrônico. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | BONFIM, D. S.; SANTANA, R. H.; BARROS, L. M. G.; CARNEIRO, M.; ALMEIDA, J. D. Isolamento de promotores em café: o método Genome Walker. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 043. p. 83.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | MENDES, I. V.; GARCIA, M. B.; SANTANA, R. H.; GLADDEN, J.; KRÜGER, R. H.; QUIRINO, B. F. Análise funcional e da diversidade de um consórcio microbiano capaz de degradar lignina. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 36.| Biblioteca(s): Embrapa Agroenergia. |
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| 9. | | GARCIA, M. B.; MENDES, I. V.; SANTANA, R. H.; KRUGER, R. H.; GLADDEN, J.; QUIRINO, B. F. Diversidade microbiana e degradação de lignina em cultura enriquecida. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 42.| Biblioteca(s): Embrapa Agroenergia. |
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| 11. | | CUNHA, M. J.; SANTANA, R. H.; VERAS, H. C. T; ABDELNOOR, R. V.; ALMEIDA, J. D.; BARROS, L. M. G. Validação de um gene de Glycine max preferencialmente expresso em folha. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. p. 73.| Biblioteca(s): Embrapa Soja. |
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| 12. | | VERAS, H. C. T.; SANTANA, R. H.; ALMEIDA, J. D. de; ABDELNOOR, R. V.; BARROS, L. M. G. Validação de um gene de soja (Glycine max) preferencialmente expresso em semente. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 040.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | VERAS, H. C. T.; SANTANA, R. H; ALMEIDA, J. D.; ABDELNOOR, R. V.; BARROS, L. M. G. Validação de um gene de soja (Glycine max) preferencialmente expresso em semente. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. p. 74.| Biblioteca(s): Embrapa Soja. |
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| 14. | | SANTANA, R. H.; VERAS, H. C. T.; CARNEIRO, V. T. de C.; ALMEIDA, J. D.; ABDELNOOR, R. V.; BARROS, L. M. G. Analise experimental da expressão diferencial de um transcrito de soja identificado através de northern eletrônico. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. p. 45.| Biblioteca(s): Embrapa Soja. |
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| 15. | | SANTANA, R. H.; DA SILVA, G. H.; BARROS, L. M. G.; CARNEIRO, M.; MARRACINI, P.; ANDRADE, A. C.; ALMEIDA, J. D. Isolamento de promotores específicos de café. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 044. p. 84.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | GUIMARÃES, H. I. P.; SANTANA, R. H.; SILVEIRA, R.; PINTO, O. H. B.; QUIRINO, B. F.; BARRETO, C. C.; BUSTAMANTE, M. M. da C.; KRÜGER, R. H. Seasonal Variations in Soil Microbiota Profile of Termite (Syntermes wheeleri) Mounds in the Brazilian Tropical Savanna. Microorganisms, v. 8, 1482, 2020. 8 Special Issue: Soil Microbiome: Biotic and Abiotic Interactions.| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | CUNHA, M. J.; SANTANA, R. H.; VERAS, H. C. T.; ABDELNOOR, R. V.; ALMEIDA, J. D. de; BARROS, L. M. G. Validação de um gene de Glycine max preferencialmente expresso em folha. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 039.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F. Bacterial diversity dynamics in microbial consortia selected for lignin utilization. PLoS ONE, v. 16, n. 9, e0255083, 2021. PDF: il. color.| Biblioteca(s): Embrapa Agroenergia. |
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| 19. | | LIMA, R. A. T.; OLIVEIRA, G. d.; SOUZA, A. A.; LOPES, F. A. C.; SANTANA, R. H.; ISTVAN, P.; QUIRINO, B. F.; BARBOSA, J.; FREITAS, S. d.; GARAY, A. V.; KRUGER, R. H. Functional and structural characterization of a novel GH3 Beta-glucosidase from the gut metagenome of the Brazilian Cerrado termite Syntermes wheeleri. International Journal of Biological Macromolecules, v. 165, Part A, p. 822-834, Dec. 2020. 165| Biblioteca(s): Embrapa Agroenergia. |
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| 20. | | COTTA, M. G.; BARROS, L. M. G.; ALMEIDA, J. D. de; SANTANA, R. H.; BARBOSA, E. A.; ALVES, G. S. C.; PAIVA, L. V.; CARNEIRO, M.; ANDRADE, A. C.; MARRACCINI, P. Lipid transfer proteins in coffee: isolation of a Coffea Orthologs, Coffea Arabica Homeologs, expression during coffee fruit development and promoter analysis in transgenic tobacco plants. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 231.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 20 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
15/09/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F. |
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Afiliação: |
ISIS VIANA MENDES, Universidade de Brasília; MARIANA BOTELHO GARCIA, Universidade Católica de Brasília; ANA CAROLINA ARAUJO BITENCOURT, Universidade de Brasília; RENATA HENRIQUE SANTANA, Instituto Federal de Brasília; PHILIPPE DE CASTRO LINS, Universidade de Brasília; RAFAELLA SILVEIRA, Universidade de Brasília; BLAKE A. SIMMONS, Joint BioEnergy Institute; JOHN M. GLADDEN, Joint BioEnergy Institute; RICARDO HENRIQUE KRUGER, Universidade de Brasília; BETANIA FERRAZ QUIRINO, CNPAE. |
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Título: |
Bacterial diversity dynamics in microbial consortia selected for lignin utilization. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
PLoS ONE, v. 16, n. 9, e0255083, 2021. |
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Descrição Física: |
PDF: il. color. |
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DOI: |
https://doi.org/10.1371/journal.pone.0255083 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications. MenosAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lign... Mostrar Tudo |
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Thesagro: |
Biotecnologia; Carbono; Composto Fenólico; Lignina. |
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Thesaurus NAL: |
Application technology; Biotechnology; Carbon; Degradation; Energy conversion; Lignin; Phenolic compounds. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226045/1/Bacterial-diversity-dynamics-in-microbial-2021.pdf
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Marc: |
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100 1 $aMENDES, I. V.
245 $aBacterial diversity dynamics in microbial consortia selected for lignin utilization.$h[electronic resource]
260 $c2021
300 $cPDF: il. color.
520 $aAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications.
650 $aApplication technology
650 $aBiotechnology
650 $aCarbon
650 $aDegradation
650 $aEnergy conversion
650 $aLignin
650 $aPhenolic compounds
650 $aBiotecnologia
650 $aCarbono
650 $aComposto Fenólico
650 $aLignina
700 1 $aGARCIA, M. B.
700 1 $aBITENCOURT, A. C. A.
700 1 $aSANTANA, R. H.
700 1 $aLINS, P. de C.
700 1 $aSILVEIRA, R.
700 1 $aSIMMONS, B. A.
700 1 $aGLADDEN, J. M.
700 1 $aKRUGER, R. H.
700 1 $aQUIRINO, B. F.
773 $tPLoS ONE$gv. 16, n. 9, e0255083, 2021.
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Embrapa Agroenergia (CNPAE) |
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