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4. | | SANTANA, J. R. F. de; CUNHA, G. A. P. da; FONSECA, N.; SOUTO, R. F. Efeito de indutores florais sobre o florescimento, frutificacao e rendimento das CVS. de manga van dyke, aden e tommy atkins no Norte de Minas Gerais Revista Brasileira de Fruticultura, v.19, n.2, p. 159-167, Cruz das Almas, 1997 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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5. | | SANTANA, J. R. F. de; CUNHA, G. A. P. da; FONSECA, N.; SOUTO, R. F. Efeito de indutores florais sobre o florescimento, frutificacao e rendimento das CVS. de manga van dyke, haden e tommy atkins no norte de Minas Gerais. II- Nitrato de potassio. Revista Brasileira de Fruticultura, v.19, n.3, p.289-296, Cruz das Almas, 1997 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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6. | | SANTANA, J. R. F. de; CUNHA, G. A. P. da; FONSECA, N.; SOUTO, R. F. Efeito de indutores de florescimento a base de nitrato sobre a malformacao floral da manga Magistra, v.8,n.9, p.43-60, Cruz das Almas, EAUFBA, 1996 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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9. | | SANTA ROSA, S.; SOUZA, F. V. D.; SANTANA, J. R. F. de. Conservação in vitro de bromélias do gênero Aechmea. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304). PDF 254. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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14. | | SILVEIRA, D. G.; CARNEIRO, C. E.; SOUZA, F. V. D.; SILVA NETO, H. P. da; SANTANA, J. R. F. de. Anatomia foliar de Neoglaziovia variegata (Arruda) Mez sob diferentes condições de cultivo. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4.; 2009, Aracaju. Ciência, inovação e sustentabilidade: [anais...]. Aracaju: Embrapa Tabuleiros Costeiros; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM. (Embrapa Tabuleiros Costeiros. Documentos, 150). Organizadores: Ana da Silva Lédo; Fernanda Vidigal D. Souza; Vivian Loges; Everton Hilo de Souza; Ana Cecília R. de Castro. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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16. | | SILVEIRA, D. G.; VIDAL, Á. M.; LEDO, C. A. da S.; SANTANA, J. R. F. de; SOUZA, F. V. D. Aspectos morfofisiológicos na pré-aclimatização in vitro e aclimatização de plantas de caroá. Revista Ciência Agronômica, v. 44, n. 3, p. 544-553, jul-set, 2013. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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17. | | SILVEIRA, D. G.; SOUZA, F. V. D.; LEDO, C. A. da S.; VIDAL, A. M.; SANTANA, J. R. F. de. Enraizamento in vitro de caroá. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4.; 2009, Aracaju. Ciência, inovação e sustentabilidade: [anais...]. Aracaju: Embrapa Tabuleiros Costeiros; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM. (Embrapa Tabuleiros Costeiros. Documentos, 150). Organizadores: Ana da Silva Lédo; Fernanda Vidigal D. Souza; Vivian Loges; Everton Hilo de Souza; Ana Cecília R. de Castro. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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19. | | SILVEIRA, D. G.; MORAIS-LINO, L. S.; SOUZA, F. V. D.; SOUZA, A. da S.; SANTANA, J. R. F. de. Embriogêneses somática em caroá. Revista Brasileira de Horticultura Ornamental, Campinas, v. 13, p.1230-1234, 2007. Suplemento. Edição dos Resumos do XVI Congresso Brasileiro de Floricultura e Plantas Ornamentais; III Congresso Brasileiro de Cultura de Tecidos e Plantas; I Simpósio de Plantas Ornamentais Nativas, Goiânia, set. 2007. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
30/10/2015 |
Data da última atualização: |
30/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
VIEIRA, L. de J.; SANTANA, J. R. F. de; ALVES, A. A. C.; LEDO, C. A. da S.; SOUZA, F. V. D. |
Afiliação: |
LÍVIA DE JESUS VIEIRA, UEFS; JOSÉ RANIERE FERREIRA DE SANTANA, UEFS; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
Título: |
Use of aniline blue stain to observing pollen tubes development in different Manihot Mill. species. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
African Journal of Agricultural Research, v. 10, n. 15, p. 1805-1809, 9 April, 2015. |
Idioma: |
Inglês |
Conteúdo: |
The lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The average pollen tube size 24 h after pollination was 2.9 mm. The studies examining pollen longevity showed that germination did not occur when the pollen grains were evaluated 24 h after anthesis. Thus, the use of aniline blue stain can be considered an effective method for observing pollen tubes development and studying the pollen morphology, longevity, viability and Manihot crossability. MenosThe lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The ... Mostrar Tudo |
Thesagro: |
Mandioca; Melhoramento vegetal; Recurso genético. |
Thesaurus NAL: |
Breeding methods; Cassava; Genetic resources. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02645naa a2200241 a 4500 001 2027633 005 2023-05-30 008 2015 bl uuuu u00u1 u #d 100 1 $aVIEIRA, L. de J. 245 $aUse of aniline blue stain to observing pollen tubes development in different Manihot Mill. species.$h[electronic resource] 260 $c2015 520 $aThe lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The average pollen tube size 24 h after pollination was 2.9 mm. The studies examining pollen longevity showed that germination did not occur when the pollen grains were evaluated 24 h after anthesis. Thus, the use of aniline blue stain can be considered an effective method for observing pollen tubes development and studying the pollen morphology, longevity, viability and Manihot crossability. 650 $aBreeding methods 650 $aCassava 650 $aGenetic resources 650 $aMandioca 650 $aMelhoramento vegetal 650 $aRecurso genético 700 1 $aSANTANA, J. R. F. de 700 1 $aALVES, A. A. C. 700 1 $aLEDO, C. A. da S. 700 1 $aSOUZA, F. V. D. 773 $tAfrican Journal of Agricultural Research$gv. 10, n. 15, p. 1805-1809, 9 April, 2015.
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