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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
02/01/2014 |
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Data da última atualização: |
12/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DOMINGUES, L. F.; GIGLIOTI, R.; FEITOSA, K. A.; FANTATTO, R. R.; RABELO, M. D.; OLIVEIRA, M. C. de S.; BECHARA, G. H.; OLIVEIRA, G. P. de; BARIONI JUNIOR, W.; CHAGAS, A. C. de S. |
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Afiliação: |
LUCIANA FERREIRA DOMINGUES, Universidade Estadual Paulista; RODRIGO GIGLIOTI, Universidade Estadual Paulista; KARINA ALVES FEITOSA, Centro Universitário Central Paulista; RAFAELA REGINA FANTATTO, Centro Universitário Central Paulista; MARCIO DIAS RABELO, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; GERVÁSIO HENRIQUE BECHARA, Centro Universitário Central Paulista; GILSON PEREIRA DE OLIVEIRA, Universidade Estadual Paulista; WALDOMIRO BARIONI JUNIOR, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
In vitro and in vivo evaluation of the activity of pineapple (Ananas comosus) on Haemonchus contortus in Santa Inês sheep. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 197, N. 1-8, p. 263-270, oct. 2013. |
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DOI: |
10.1016/j.vetpar.2013.04.031 |
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Idioma: |
Inglês |
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Conteúdo: |
The development of resistance to anthelmintics has prompted research into alternative methods of controlling intestinal nematodes in ruminants. This study aimed to assess the activity of Ananas comosus on Haemonchus contortus in Santa Inês sheep. The aqueous extract of pineapple skin (AEPS), bromelain from pineapple stems (B4882) and residue from pineapple processing was evaluated in in vitro and in vivo tests. The enzymatic activity of substances was analyzed by the azocasein method. The egg hatch test (EHT) and larval development test (LDT) were performed using the Embrapa2010 isolate of H. contortus. In the in vivo test, 36 sheep artificially infected with H. contortus were divided into six groups: G1: 2 g/kg BW of the aqueous extract administered for three days; G2: 2 g/kg BW of the industrial pineapple residue for 60 days; G3: 180 mg/animal of bromelain in a single dose; G4: negative control I; G5: positive control (levamisole phosphate); and G6: negative control II. The eggs per gram (EPG) in the feces were counted till 28 days after treatment. LC?? and LC?? were obtained by the probit procedure, while the in vivo test results were analyzed by GLM. The aqueous extract in the in vitro and in vivo test, the bromelain and industrial residue presented 0.102, 0.157, 1.864 and 0.048 enzyme units/mL, respectively. In the egg hatch test, the LC?? and LC?? were respectively 31 and 81 mg/mL for the aqueous extract and 0.50 and 2 mg/mL for bromelain. In the larval development test, the LC?? and LC?? were respectively 1.7 and 7.3 mg/mL for the aqueous extract and 0.019 and 0.086 mg/mL for bromelain. In the in vivo test, the general efficacies of the treatments in relation to the negative control were 22.6%, 42.2%, 3.65% and 89% for the aqueous extract, industrial pineapple residue, bromelain and positive control respectively. The transformed EPG values were 3.19 ± 0.59, 3.32 ± 0.25, 2.85 ± 0.66, 3.44 ± 0.50, 2.28 ± 0.93 and 2.75 ± 0.94 for the aqueous extract, industrial residue, bromelain, negative control I, positive control and negative control II respectively. The results for all the treated groups differed significantly (p<0.05) from the positive control, and although the residue presented efficacy of 42.2%, there was no statistical difference (p>0.05) in relation to the negative control. Therefore, both the aqueous extract and bromelain were effective in vitro, but showed reduced anthelmintic efficacy in vivo. For the pineapple residue, the 42.2% in vivo efficacy in reducing the EPG and the possibility of reducing environmental contamination through reuse of industrial residue indicate it can also be useful for control of this parasite. MenosThe development of resistance to anthelmintics has prompted research into alternative methods of controlling intestinal nematodes in ruminants. This study aimed to assess the activity of Ananas comosus on Haemonchus contortus in Santa Inês sheep. The aqueous extract of pineapple skin (AEPS), bromelain from pineapple stems (B4882) and residue from pineapple processing was evaluated in in vitro and in vivo tests. The enzymatic activity of substances was analyzed by the azocasein method. The egg hatch test (EHT) and larval development test (LDT) were performed using the Embrapa2010 isolate of H. contortus. In the in vivo test, 36 sheep artificially infected with H. contortus were divided into six groups: G1: 2 g/kg BW of the aqueous extract administered for three days; G2: 2 g/kg BW of the industrial pineapple residue for 60 days; G3: 180 mg/animal of bromelain in a single dose; G4: negative control I; G5: positive control (levamisole phosphate); and G6: negative control II. The eggs per gram (EPG) in the feces were counted till 28 days after treatment. LC?? and LC?? were obtained by the probit procedure, while the in vivo test results were analyzed by GLM. The aqueous extract in the in vitro and in vivo test, the bromelain and industrial residue presented 0.102, 0.157, 1.864 and 0.048 enzyme units/mL, respectively. In the egg hatch test, the LC?? and LC?? were respectively 31 and 81 mg/mL for the aqueous extract and 0.50 and 2 mg/mL for bromelain. In the larval development tes... Mostrar Tudo |
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Palavras-Chave: |
Aqueous extract; Bromelain; Industrial residue. |
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Thesaurus Nal: |
Cysteine proteinases; Phytotherapy. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03637naa a2200301 a 4500
001 1974794
005 2023-05-12
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vetpar.2013.04.031$2DOI
100 1 $aDOMINGUES, L. F.
245 $aIn vitro and in vivo evaluation of the activity of pineapple (Ananas comosus) on Haemonchus contortus in Santa Inês sheep.$h[electronic resource]
260 $c2013
520 $aThe development of resistance to anthelmintics has prompted research into alternative methods of controlling intestinal nematodes in ruminants. This study aimed to assess the activity of Ananas comosus on Haemonchus contortus in Santa Inês sheep. The aqueous extract of pineapple skin (AEPS), bromelain from pineapple stems (B4882) and residue from pineapple processing was evaluated in in vitro and in vivo tests. The enzymatic activity of substances was analyzed by the azocasein method. The egg hatch test (EHT) and larval development test (LDT) were performed using the Embrapa2010 isolate of H. contortus. In the in vivo test, 36 sheep artificially infected with H. contortus were divided into six groups: G1: 2 g/kg BW of the aqueous extract administered for three days; G2: 2 g/kg BW of the industrial pineapple residue for 60 days; G3: 180 mg/animal of bromelain in a single dose; G4: negative control I; G5: positive control (levamisole phosphate); and G6: negative control II. The eggs per gram (EPG) in the feces were counted till 28 days after treatment. LC?? and LC?? were obtained by the probit procedure, while the in vivo test results were analyzed by GLM. The aqueous extract in the in vitro and in vivo test, the bromelain and industrial residue presented 0.102, 0.157, 1.864 and 0.048 enzyme units/mL, respectively. In the egg hatch test, the LC?? and LC?? were respectively 31 and 81 mg/mL for the aqueous extract and 0.50 and 2 mg/mL for bromelain. In the larval development test, the LC?? and LC?? were respectively 1.7 and 7.3 mg/mL for the aqueous extract and 0.019 and 0.086 mg/mL for bromelain. In the in vivo test, the general efficacies of the treatments in relation to the negative control were 22.6%, 42.2%, 3.65% and 89% for the aqueous extract, industrial pineapple residue, bromelain and positive control respectively. The transformed EPG values were 3.19 ± 0.59, 3.32 ± 0.25, 2.85 ± 0.66, 3.44 ± 0.50, 2.28 ± 0.93 and 2.75 ± 0.94 for the aqueous extract, industrial residue, bromelain, negative control I, positive control and negative control II respectively. The results for all the treated groups differed significantly (p<0.05) from the positive control, and although the residue presented efficacy of 42.2%, there was no statistical difference (p>0.05) in relation to the negative control. Therefore, both the aqueous extract and bromelain were effective in vitro, but showed reduced anthelmintic efficacy in vivo. For the pineapple residue, the 42.2% in vivo efficacy in reducing the EPG and the possibility of reducing environmental contamination through reuse of industrial residue indicate it can also be useful for control of this parasite.
650 $aCysteine proteinases
650 $aPhytotherapy
653 $aAqueous extract
653 $aBromelain
653 $aIndustrial residue
700 1 $aGIGLIOTI, R.
700 1 $aFEITOSA, K. A.
700 1 $aFANTATTO, R. R.
700 1 $aRABELO, M. D.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aBECHARA, G. H.
700 1 $aOLIVEIRA, G. P. de
700 1 $aBARIONI JUNIOR, W.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv. 197, N. 1-8, p. 263-270, oct. 2013.
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Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
25/08/2014 |
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Data da última atualização: |
17/05/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
EGITO, A. S. do; POMPEU, R. C. F. F.; ANDRADE, F. P. de; FEITOSA, T. S.; RAMOS, M. V.; SALLES, H. O. |
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Afiliação: |
ANTONIO SILVIO DO EGITO, CNPC; ROBERTO CLAUDIO FERNANDES F POMPEU, CNPC; Francisco Pereira de Andrade; Tibério Sousa Feitosa; Márcio Viana Ramos; HEVILA OLIVEIRA SALLES, CNPC. |
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Título: |
Hidrólise de proteínas tóxicas de sementes de mamona utilizando látex vegetal como fonte proteolítica. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MAMONA, 6.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 3., 2014, Fortaleza. Energia e segurança alimentar na agricultura familiar: anais. Campina Grande, PB: Embrapa Algodão, 2014. 1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Biorremediação; Destoxificação; Detoxification; Variedade BRS-Energia. |
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Thesagro: |
Biocombustível; Digestibilidade; Enzima; Extrato vegetal; Hidrólise; Latex; Mamona; Protease; Ricinus communis; Semente; Substância tóxica; Torta. |
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Thesaurus NAL: |
Biofuels; Calotropis procera; Castor beans; Digestibility; Enzymatic hydrolysis; Ricin; Toxic substances. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107230/1/rac-Hidrolise.pdf
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Marc: |
LEADER 01391nam a2200433 a 4500
001 1993310
005 2022-05-17
008 2014 bl uuuu u00u1 u #d
100 1 $aEGITO, A. S. do
245 $aHidrólise de proteínas tóxicas de sementes de mamona utilizando látex vegetal como fonte proteolítica.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MAMONA, 6.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 3., 2014, Fortaleza. Energia e segurança alimentar na agricultura familiar: anais. Campina Grande, PB: Embrapa Algodão, 2014. 1 CD-ROM.$c2014
650 $aBiofuels
650 $aCalotropis procera
650 $aCastor beans
650 $aDigestibility
650 $aEnzymatic hydrolysis
650 $aRicin
650 $aToxic substances
650 $aBiocombustível
650 $aDigestibilidade
650 $aEnzima
650 $aExtrato vegetal
650 $aHidrólise
650 $aLatex
650 $aMamona
650 $aProtease
650 $aRicinus communis
650 $aSemente
650 $aSubstância tóxica
650 $aTorta
653 $aBiorremediação
653 $aDestoxificação
653 $aDetoxification
653 $aVariedade BRS-Energia
700 1 $aPOMPEU, R. C. F. F.
700 1 $aANDRADE, F. P. de
700 1 $aFEITOSA, T. S.
700 1 $aRAMOS, M. V.
700 1 $aSALLES, H. O.
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