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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
17/12/1997 |
Data da última atualização: |
07/11/2016 |
Autoria: |
GRACA, L. R.; COUTO, L.; BETTERS, D. R. |
Afiliação: |
Graca, Embrapa-CNPF. |
Título: |
Agroforestry systems with bracatinga in southern Brazil: technical and economic perspectives. |
Ano de publicação: |
1994 |
Fonte/Imprenta: |
In: NORTH AMERICAN AGROFORESTRY CONFERENCE, 3., 1993, Ames. Proceedings... Ames: Iowa State University. Department of Forestry, College of Agriculture, 1994. p.3337-341. |
Idioma: |
Inglês |
Thesagro: |
Agrossilvicultura; Bracatinga; Mimosa Scabrella. |
Thesaurus Nal: |
agroforestry. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00656nam a2200169 a 4500 001 1303424 005 2016-11-07 008 1994 bl uuuu u00u1 u #d 100 1 $aGRACA, L. R. 245 $aAgroforestry systems with bracatinga in southern Brazil$btechnical and economic perspectives.$h[electronic resource] 260 $aIn: NORTH AMERICAN AGROFORESTRY CONFERENCE, 3., 1993, Ames. Proceedings... Ames: Iowa State University. Department of Forestry, College of Agriculture, 1994. p.3337-341.$c3337 650 $aagroforestry 650 $aAgrossilvicultura 650 $aBracatinga 650 $aMimosa Scabrella 700 1 $aCOUTO, L. 700 1 $aBETTERS, D. R.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
29/03/2019 |
Data da última atualização: |
21/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 3 |
Autoria: |
CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M. |
Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MICHELE MUNK, UFJF; JOSE NELIO SALES, UFLA; SABINE WOHLRES-VIANA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
Título: |
Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
JBRA Assisted Reproduction, v. 23, n. 1, p. 7-14, 2019. |
DOI: |
10.5935/1518-0557.20180084 |
Idioma: |
Inglês |
Notas: |
Na publicação: Luiz Sergio Almeida Camargo. |
Conteúdo: |
Objective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool. MenosObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of p... Mostrar Tudo |
Palavras-Chave: |
In vivo maturation; MRNA; Ovum pick-up. |
Thesaurus NAL: |
Gene expression. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195183/1/jbra-23-01-0007.pdf
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Marc: |
LEADER 02498naa a2200253 a 4500 001 2107759 005 2022-06-21 008 2019 bl uuuu u00u1 u #d 024 7 $a10.5935/1518-0557.20180084$2DOI 100 1 $aCAMARGO, L. S. de A. 245 $aDifferential gene expression between in vivo and in vitro maturation$ba comparative study with bovine oocytes derived from the same donor pool.$h[electronic resource] 260 $c2019 500 $aNa publicação: Luiz Sergio Almeida Camargo. 520 $aObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool. 650 $aGene expression 653 $aIn vivo maturation 653 $aMRNA 653 $aOvum pick-up 700 1 $aMUNK, M. 700 1 $aSALES, J. N. 700 1 $aWOHLRES-VIANA, S. 700 1 $aQUINTAO, C. C. R. 700 1 $aVIANA, J. H. M. 773 $tJBRA Assisted Reproduction$gv. 23, n. 1, p. 7-14, 2019.
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