| |
|
|
| Registros recuperados : 6 | |
| 2. |  | CARVALHO, W. A.; GERN, J. C.; GUIMARAES, A. S.; BRAYNER, R.; GUELBER, G. T.; SALES, E. B.; MACHADO, M. A.; SILVA, S. R.; BRANDAO, H. de M. Toxicity of barium titanate in bovine fibroblasts. In: ENCONTRO DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS, SBPMAT, 12., 2013, Campos do Jordão. Resumos. Campos do Jordão: SBPMat, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 4. | | CASTELÃO, A. B. C.; NISHIBE, C.; MOURA, A.; ALENCAR, A. P. de; ISSA, M. de A.; HODON, M. A.; MOTA, P. M. P. C.; SALES, E. B.; FONSECA JÚNIOR, A. A.; ALMEIDA, N. F.; ARAUJO, F. R. Draft Genome Sequence of Mycobacterium bovis Strain AN5, Used for Production of Purified Protein Derivative. Genome Announcements, v. 2, n. 2, mar./abr 2014, p. 1-2.| Biblioteca(s): Embrapa Gado de Corte. |
|  |
| 5. | | SALES, M. L.; FONSECA JUNIOR, A. A.; SALES, E. B.; COTTORELLO, A. C. P.; ISSA, M. A.; HODON, M. A.; SOARES FILHO, P. M.; RAMALHO, A. K.; SILVA, M. R.; LAGE, A. P.; HEINEMANN, M. B. Evaluation of molecular markers for the diagnosis of Mycobacterium bovis Folia Microbiologica, v. 59, n. 5, p. 433-438, 2014.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 6. | | SILVA, M. R.; DUCH, A. A. S.; LAGE, R. T. P. de A.; FARIA, L. S. de; MENEZES, L. D. M.; RIBEIRO, J. B.; SOUZA, G. N. de; SOARES FILHO, P. M.; PREIS, I. S.; SALES, E. B.; SOUZA, P. G. de; ARAUJO, F. R.; GUIMARÃES, R. J. de P. S. e; MENDES, T.; PETTAN-BREWER, C.; FONSECA-JÚNIOR, A. A. Recovery of Brucella in raw milk Minas artisanal cheese approved for consumption by official inspection agency in Brazil: assessment of prevalence and risk factors through One Health integrated approaches. Transactions of The Royal Society of Tropical Medicine and Hygiene, trac083, 2022.| Biblioteca(s): Embrapa Gado de Corte; Embrapa Gado de Leite. |
| |
| Registros recuperados : 6 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Suínos e Aves. |
|
Data corrente: |
22/03/2012 |
|
Data da última atualização: |
22/03/2012 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 5 |
|
Autoria: |
FONSECA JÚNIOR, A. A.; MAGALHÃES, C. G.; SALES, E. B.; D'AMBROS, R. M.; ZANELLA, J. R. C.; HEINEMANN, M. B.; LEITE, R. C.; REIS, J. K. P. dos. |
|
Afiliação: |
ANTONIO AUGUSTO FONSECA JÚNIOR, LANAGRO; CRISTINA GONÇALVES MAGALHÃES, LANAGRO; ERICA BRAVO SALES, LANAGRO; REGIA MARIA D'AMBROS, CEDISA; JANICE REIS CIACCI ZANELLA, CNPSA; MARCOS BRYAN HEINEMANN, UFMG; ROMULO CERQUEIRA LEITE, UFMG; JENNER KARLISSON PIMENTA DOS REIS, UFMG. |
|
Título: |
Genotyping of the Pseudorabies vírus by multiplex PCR followed by restriction enzyme analysis. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
ISRN Microbiology, v. 2011. Doi:10.5402/2011/458294. |
|
Idioma: |
Inglês |
|
Notas: |
Projeto/Plano de Ação: 03.09.00.046. |
|
Conteúdo: |
Suid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky?s disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1. |
|
Palavras-Chave: |
PCR. |
|
Thesagro: |
Genótipo; Vírus. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01560naa a2200253 a 4500
001 1919948
005 2012-03-22
008 2011 bl uuuu u00u1 u #d
100 1 $aFONSECA JÚNIOR, A. A.
245 $aGenotyping of the Pseudorabies vírus by multiplex PCR followed by restriction enzyme analysis.$h[electronic resource]
260 $c2011
500 $aProjeto/Plano de Ação: 03.09.00.046.
520 $aSuid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky?s disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1.
650 $aGenótipo
650 $aVírus
653 $aPCR
700 1 $aMAGALHÃES, C. G.
700 1 $aSALES, E. B.
700 1 $aD'AMBROS, R. M.
700 1 $aZANELLA, J. R. C.
700 1 $aHEINEMANN, M. B.
700 1 $aLEITE, R. C.
700 1 $aREIS, J. K. P. dos
773 $tISRN Microbiology$gv. 2011. Doi:10.5402/2011/458294.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
