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Registro Completo |
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
25/11/2009 |
Data da última atualização: |
20/10/2010 |
Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
Autoria: |
SILVA, L. P. da. |
Título: |
Caracterização morfológica, fisiológica, molecular e patogênica de isolados de Colletotrichum spp. em diferentes hospedeiros. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Pelotas: Universidade Federal de Pelotas, 2008. |
Páginas: |
149 f. |
Idioma: |
Português |
Notas: |
Tese (Doutorado em Fitossanidade) - Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas. Co-Orientadores: Bernardo Ueno e Juliana Degenhardt ; Embrapa Clima Temperado. |
Palavras-Chave: |
Atividade proteolítica; Capsicum spp; Inoculação cruzada; PCR; RFLP. |
Thesagro: |
Colletotrichum Gloeosporioides. |
Thesaurus Nal: |
Colletotrichum acutatum; Colletotrichum capsici. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00871nam a2200217 a 4500 001 1746865 005 2010-10-20 008 2008 bl uuuu m 00u1 u #d 100 1 $aSILVA, L. P. da 245 $aCaracterização morfológica, fisiológica, molecular e patogênica de isolados de Colletotrichum spp. em diferentes hospedeiros. 260 $aPelotas: Universidade Federal de Pelotas$c2008 300 $a149 f. 500 $aTese (Doutorado em Fitossanidade) - Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas. Co-Orientadores: Bernardo Ueno e Juliana Degenhardt ; Embrapa Clima Temperado. 650 $aColletotrichum acutatum 650 $aColletotrichum capsici 650 $aColletotrichum Gloeosporioides 653 $aAtividade proteolítica 653 $aCapsicum spp 653 $aInoculação cruzada 653 $aPCR 653 $aRFLP
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
25/09/2020 |
Data da última atualização: |
25/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
ALKIMIM, E. R.; CAIXETA, E. T.; SOUSA, T. V.; SILVA, F. L. da; SAKYAMA, N. S.; ZAMBOLIM, L. |
Afiliação: |
EMILLY RUAS ALKIMIM, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; TIAGO VIEIRA SOUSA, UNIVERSIDADE FEDERAL DE VIÇOSA; FELIPE LOPES DA SILVA, UNIVERSIDADE FEDERAL DE VIÇOSA; NEY SUSSUMU SAKIYAMA, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA. |
Título: |
High-throughput targeted genotyping using next-generation sequencing applied in Coffea canephora breeding. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Euphytica, v. 214, p. 50, 2018. |
DOI: |
https://doi.org/10.1007/s10681-018-2126-2 |
Idioma: |
Inglês |
Conteúdo: |
The use of molecular markers to detect polymorphism at DNA level is one of the most significant developments in molecular biology techniques. With the development of new next-generation sequencing technologies, the discovery of SNP became easier and faster, and the costs of data point were reduced. The development and use of SNP markers for coffee have provided new perspectives for the evaluation of genetic diversity and population structure via different statistical approaches. In this study, 72 Coffea canephora genotypes were analyzed to identify the SNP markers and apply them to genetic studies and selection of parents/hybrids in genetic breeding. As many as 117,450 SNP were identified using the RAPiD Genomics platform. After quality analyses, 33,485 SNP were validated for analyses of genetic diversity and population structure. Genotypes were separated based on their varietal groups, and Hybrids were differentiated using the clustering and Bayesian approach. Coffee accessions mistakenly identified in the germplasm and breeding program were detected. The Conilon varietal group presented the lowest genetic dissimilarity values, suggesting the introduction of new accessions in the germplasm bank. The highest genetic distances values were observed among genotypes of the heterotic groups (Conilon and Robusta). The markers were efficient in evaluating the genetic diversity and population structure of C. canephora. Promising crosses were selected within and between the varietal groups. Hybrids with greater genetic distances were selected, which were important for C. canephora breeding programs. MenosThe use of molecular markers to detect polymorphism at DNA level is one of the most significant developments in molecular biology techniques. With the development of new next-generation sequencing technologies, the discovery of SNP became easier and faster, and the costs of data point were reduced. The development and use of SNP markers for coffee have provided new perspectives for the evaluation of genetic diversity and population structure via different statistical approaches. In this study, 72 Coffea canephora genotypes were analyzed to identify the SNP markers and apply them to genetic studies and selection of parents/hybrids in genetic breeding. As many as 117,450 SNP were identified using the RAPiD Genomics platform. After quality analyses, 33,485 SNP were validated for analyses of genetic diversity and population structure. Genotypes were separated based on their varietal groups, and Hybrids were differentiated using the clustering and Bayesian approach. Coffee accessions mistakenly identified in the germplasm and breeding program were detected. The Conilon varietal group presented the lowest genetic dissimilarity values, suggesting the introduction of new accessions in the germplasm bank. The highest genetic distances values were observed among genotypes of the heterotic groups (Conilon and Robusta). The markers were efficient in evaluating the genetic diversity and population structure of C. canephora. Promising crosses were selected within and between the varietal ... Mostrar Tudo |
Thesaurus NAL: |
Hybrids; Polymorphism; Population structure. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216202/1/Alkimim-et-al-2018-1.pdf
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Marc: |
LEADER 02307naa a2200229 a 4500 001 2125108 005 2020-09-25 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s10681-018-2126-2$2DOI 100 1 $aALKIMIM, E. R. 245 $aHigh-throughput targeted genotyping using next-generation sequencing applied in Coffea canephora breeding.$h[electronic resource] 260 $c2018 520 $aThe use of molecular markers to detect polymorphism at DNA level is one of the most significant developments in molecular biology techniques. With the development of new next-generation sequencing technologies, the discovery of SNP became easier and faster, and the costs of data point were reduced. The development and use of SNP markers for coffee have provided new perspectives for the evaluation of genetic diversity and population structure via different statistical approaches. In this study, 72 Coffea canephora genotypes were analyzed to identify the SNP markers and apply them to genetic studies and selection of parents/hybrids in genetic breeding. As many as 117,450 SNP were identified using the RAPiD Genomics platform. After quality analyses, 33,485 SNP were validated for analyses of genetic diversity and population structure. Genotypes were separated based on their varietal groups, and Hybrids were differentiated using the clustering and Bayesian approach. Coffee accessions mistakenly identified in the germplasm and breeding program were detected. The Conilon varietal group presented the lowest genetic dissimilarity values, suggesting the introduction of new accessions in the germplasm bank. The highest genetic distances values were observed among genotypes of the heterotic groups (Conilon and Robusta). The markers were efficient in evaluating the genetic diversity and population structure of C. canephora. Promising crosses were selected within and between the varietal groups. Hybrids with greater genetic distances were selected, which were important for C. canephora breeding programs. 650 $aHybrids 650 $aPolymorphism 650 $aPopulation structure 700 1 $aCAIXETA, E. T. 700 1 $aSOUSA, T. V. 700 1 $aSILVA, F. L. da 700 1 $aSAKYAMA, N. S. 700 1 $aZAMBOLIM, L. 773 $tEuphytica$gv. 214, p. 50, 2018.
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Embrapa Café (CNPCa) |
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