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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
18/02/2016 |
Data da última atualização: |
29/03/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DEUS, K. E. de; LANNA, A. C.; ABREU, F. R. M.; SILVEIRA, R. D. D.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO, R. P. |
Afiliação: |
KARINNE EVARISTO DE DEUS, UFG; ANNA CRISTINA LANNA, CNPAF; FERNANDA RAQUEL MARTINS ABREU, UFG; RICARDO DIOGENES DIAS SILVEIRA; WENDELL JACINTO PEREIRA, UFG; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
Título: |
Molecular and biochemical characterization of superoxide dismutase (SOD) in upland rice under drought. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Australian Journal of Crop Science, v. 9, n. 8, p. 744-753, 2015. |
ISSN: |
1835-2707 |
Idioma: |
Inglês |
Conteúdo: |
Drought is a major cause of reduced yield in upland rice farming in many regions of the world and cause an increased production of reactive oxygen species (ROS), followed by activation of a complex antioxidant system. In this study, the superoxide dismutase (SOD) enzymatic activity and the gene expression levels of eight isoforms of SOD were evaluated in drought-tolerant and drought-sensitive varieties of upland rice, Oryza sativa japonica (the Primavera and Douradão cultivars), including shoot and root tissues in two stages of plant development, grown under normal and restricted irrigation. The SOD activity was determined by spectrophotometric method and the gene expression by qPCR experiments. In the tolerant cultivar, SOD enzymatic activity increased significantly (p ≤ 0.05) only in the root tissue in the reproductive stage (268.00 SOD UN mg-1), whereas in the sensitive cultivar, SOD increased in the leaf (112.17 SOD UN mg-1) and root (172.56 SOD UN mg-1) tissues in the reproductive stage. The genes CuZnSOD4 and MnSOD showed significant increases in expression (p ≤ 0.05) in both tissues and developmental stages in the tolerant cultivar under drought, whereas FeSOD1 showed increased expression (p ≤ 0.05) only in the tolerant genotype (vegetative stage/leaf and root) under water restriction. The different patterns of SOD activity and/or gene expression in upland rice plants should be strongly considered to elucidate the cellular mechanisms of drought tolerance; these findings may ultimately inform breeding programs aimed at more efficiently developing cultivars that are better adapted to areas prone to water deficits. MenosDrought is a major cause of reduced yield in upland rice farming in many regions of the world and cause an increased production of reactive oxygen species (ROS), followed by activation of a complex antioxidant system. In this study, the superoxide dismutase (SOD) enzymatic activity and the gene expression levels of eight isoforms of SOD were evaluated in drought-tolerant and drought-sensitive varieties of upland rice, Oryza sativa japonica (the Primavera and Douradão cultivars), including shoot and root tissues in two stages of plant development, grown under normal and restricted irrigation. The SOD activity was determined by spectrophotometric method and the gene expression by qPCR experiments. In the tolerant cultivar, SOD enzymatic activity increased significantly (p ≤ 0.05) only in the root tissue in the reproductive stage (268.00 SOD UN mg-1), whereas in the sensitive cultivar, SOD increased in the leaf (112.17 SOD UN mg-1) and root (172.56 SOD UN mg-1) tissues in the reproductive stage. The genes CuZnSOD4 and MnSOD showed significant increases in expression (p ≤ 0.05) in both tissues and developmental stages in the tolerant cultivar under drought, whereas FeSOD1 showed increased expression (p ≤ 0.05) only in the tolerant genotype (vegetative stage/leaf and root) under water restriction. The different patterns of SOD activity and/or gene expression in upland rice plants should be strongly considered to elucidate the cellular mechanisms of drought toler... Mostrar Tudo |
Palavras-Chave: |
Enzymatic activity. |
Thesagro: |
Arroz; Oryza sativa; Resistencia a seca. |
Thesaurus Nal: |
Gene expression; Oxidative stress; Rice; Superoxide dismutase; Water stress. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139323/1/CNPAF-2015-ajcs.pdf
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Marc: |
LEADER 02567naa a2200313 a 4500 001 2037521 005 2016-03-29 008 2015 bl uuuu u00u1 u #d 022 $a1835-2707 100 1 $aDEUS, K. E. de 245 $aMolecular and biochemical characterization of superoxide dismutase (SOD) in upland rice under drought.$h[electronic resource] 260 $c2015 520 $aDrought is a major cause of reduced yield in upland rice farming in many regions of the world and cause an increased production of reactive oxygen species (ROS), followed by activation of a complex antioxidant system. In this study, the superoxide dismutase (SOD) enzymatic activity and the gene expression levels of eight isoforms of SOD were evaluated in drought-tolerant and drought-sensitive varieties of upland rice, Oryza sativa japonica (the Primavera and Douradão cultivars), including shoot and root tissues in two stages of plant development, grown under normal and restricted irrigation. The SOD activity was determined by spectrophotometric method and the gene expression by qPCR experiments. In the tolerant cultivar, SOD enzymatic activity increased significantly (p ≤ 0.05) only in the root tissue in the reproductive stage (268.00 SOD UN mg-1), whereas in the sensitive cultivar, SOD increased in the leaf (112.17 SOD UN mg-1) and root (172.56 SOD UN mg-1) tissues in the reproductive stage. The genes CuZnSOD4 and MnSOD showed significant increases in expression (p ≤ 0.05) in both tissues and developmental stages in the tolerant cultivar under drought, whereas FeSOD1 showed increased expression (p ≤ 0.05) only in the tolerant genotype (vegetative stage/leaf and root) under water restriction. The different patterns of SOD activity and/or gene expression in upland rice plants should be strongly considered to elucidate the cellular mechanisms of drought tolerance; these findings may ultimately inform breeding programs aimed at more efficiently developing cultivars that are better adapted to areas prone to water deficits. 650 $aGene expression 650 $aOxidative stress 650 $aRice 650 $aSuperoxide dismutase 650 $aWater stress 650 $aArroz 650 $aOryza sativa 650 $aResistencia a seca 653 $aEnzymatic activity 700 1 $aLANNA, A. C. 700 1 $aABREU, F. R. M. 700 1 $aSILVEIRA, R. D. D. 700 1 $aPEREIRA, W. J. 700 1 $aBRONDANI, C. 700 1 $aVIANELLO, R. P. 773 $tAustralian Journal of Crop Science$gv. 9, n. 8, p. 744-753, 2015.
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Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
23/02/1996 |
Data da última atualização: |
07/10/2016 |
Autoria: |
OLIVEIRA, F. de; SAITO, M. L.; GARCIA, L. de O. |
Afiliação: |
FERNANDO DE OLIVEIRA, UNIVERSIDADE SÃO FRANCISCO; MARIA LUCIA SAITO, CNPMA; LUCAS DE OLIVEIRA GARCIA, UNIVERSIDADE SÃO FRANCISCO. |
Título: |
Caracterização farmacognóstica da droga e do extrato fluido de mentrasto: Ageratum conyzoides L. |
Ano de publicação: |
1993 |
Fonte/Imprenta: |
Lecta, Bragança Paulista, v.11, n.1, p.63-100, 1993. |
Idioma: |
Português |
Conteúdo: |
A especie vegetal Argentum conyzoides L., empregada popularmente como antidiarreica, antiespasmódica, carminativa, febrifuga, antirreumática teve sua propriedade anti inflamatória comprovada cientificamente, foi estudada morfológica e anatomicamente com vistas a sua caracterização farmacognostica . O extrato fluido da referida planta foi analisado por cromatografia em camada delgada, através de diversos sistemas cromatográficas, com vistas a fornecer subsídios ao controle de qualidade deste insumo farmacêutico. |
Palavras-Chave: |
Ageratum conyzoides l; Antidesinterica; Antidiarrhea; Antiespasmodica; Antifisetica; Antiinflamatoria; Antiphologistic; Antispasmodic; Aperiente; Caracterizacao farmacognostica; Essential oil; Febrifuga; Febrifugal; Fluid extracted; Medicinal plant; Mentraste; Mentrasto; Pharmacognostic characterization; Tonica. |
Thesagro: |
Óleo Essencial; Planta Medicinal. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148508/1/1992SP009-Saito-CaracterizacaoFamacognostica-3030.PDF
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Marc: |
LEADER 01653naa a2200397 a 4500 001 1012409 005 2016-10-07 008 1993 bl uuuu u00u1 u #d 100 1 $aOLIVEIRA, F. de 245 $aCaracterização farmacognóstica da droga e do extrato fluido de mentrasto$bAgeratum conyzoides L.$h[electronic resource] 260 $c1993 520 $aA especie vegetal Argentum conyzoides L., empregada popularmente como antidiarreica, antiespasmódica, carminativa, febrifuga, antirreumática teve sua propriedade anti inflamatória comprovada cientificamente, foi estudada morfológica e anatomicamente com vistas a sua caracterização farmacognostica . O extrato fluido da referida planta foi analisado por cromatografia em camada delgada, através de diversos sistemas cromatográficas, com vistas a fornecer subsídios ao controle de qualidade deste insumo farmacêutico. 650 $aÓleo Essencial 650 $aPlanta Medicinal 653 $aAgeratum conyzoides l 653 $aAntidesinterica 653 $aAntidiarrhea 653 $aAntiespasmodica 653 $aAntifisetica 653 $aAntiinflamatoria 653 $aAntiphologistic 653 $aAntispasmodic 653 $aAperiente 653 $aCaracterizacao farmacognostica 653 $aEssential oil 653 $aFebrifuga 653 $aFebrifugal 653 $aFluid extracted 653 $aMedicinal plant 653 $aMentraste 653 $aMentrasto 653 $aPharmacognostic characterization 653 $aTonica 700 1 $aSAITO, M. L. 700 1 $aGARCIA, L. de O. 773 $tLecta, Bragança Paulista$gv.11, n.1, p.63-100, 1993.
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