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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/03/2009 |
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Data da última atualização: |
06/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRUSKIEWICH, R.; SENGER, M.; DAVENPORT, G.; RUIZ, M.; ROUARD, M.; HAZEKAMP, T.; TAKEYA, M.; DOI, K.; SATOH, K.; COSTA, M.; SIMON, R.; BALAJI, J.; AKINTUNDE, A.; MAULEON, R.; WANCHANA, S.; SHAH, T.; ANACLETO, M.; PORTUGAL, A.; ULAT, V. J.; THONGJUEA, S.; BRAAK, K.; RITTER, S.; DEREEPER, M.; SKOFIC, M.; ROJAS, E.; MARTINS, N.; PAPPAS, G.; ALAMBAN, R.; ALMODIEL, R.; BARBOZA, L. H.; DETRAS, J.; MANANSALA, K.; MENDOZA, M. J.; MORALES, J.; PERALTA, B.; VALERIO, R.; ZHANG, Y.; GREGORIO, S.; HERMOCILLA, J.; ECHAVEZ, M.; YAP, J. M.; FARMER, A.; SCHILTZ, G.; LEE, J.; CASSTEVENS, T.; JAISWAL, P.; MEINTJES, A.; WILKINSON, M.; GOOD, B.; WAGNER, J.; MORRIS, J.; MARSHALL, D.; COLLINS, A.; KIKUCHI, S.; METZ, T.; MCLAREN, G.; HINTUM, T. van. |
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Afiliação: |
Richard Bruskiewich IRRI Filipinas; Martin Senger IRRI Filipinas; Guy Davenport CIMMYT México; Manuel Ruiz CIRAD França; Mathieu Rouard Fiumicino Italia; Tom Hazekamp Fiumicino Italia; Masaru Takeya NIAS Japão; Koji Doi NIAS Japão; Kouji Satoh NIAS Japão; Marcos Costa, Embrapa Recursos Genéticos e Biotecnologia; Reinhard Simon CIP Peru; Jayashree Balaji, India; Akinnola Akintunde, Siria; Ramil Mauleon IRRI Filipinas; Samart Wanchana IRRI Filipinas; Trushar Shah CIMMYT México; Mylah Anacleto IRRI Filipinas; Arllet Portugal IRRI Filipinas; Victor Jun Ulat IRRI Filipinas; Supat Thongjuea, Tailandia; Kyle Braak CIMMYT México; Sebastian Ritter CIMMYT México; Alexis Dereeper CIRAD França; Milko Skofic Fiumicino Italia; Edwin Rojas CIP Peru; Natália Florêncio Martins, Embrapa Recursos Genéticos e Biotecnologia; Georgios Joannis Pappas Junior, Embrapa Recursos Genéticos e Biotecnologia; Ryan Alamban IRRI Filipinas; Roque Almodiel IRRI Filipinas; Lord Hendrix Barboza IRRI Filipinas; Jeffrey Detras IRRI Filipinas; Kevin Manansala IRRI Filipinas; Michael Jonathan Mendoza IRRI Filipinas; Jeffrey Morales IRRI Filipinas; Barry Peralta IRRI Filipinas; Rowena Valerio IRRI Filipinas; Yi Zhang IRRI Filipinas; Sérgio Gregorio IRRI Filipinas; Joseph Hermocilla IRRI Filipinas; Michael Echavez IRRI Filipinas; Jan Michael Yap IRRI Filipinas; Andrew Farmer, Estados Unidos; Gary Schiltz, Estados Unidos; Jennifer Lee, Inglaterra; Terry Casstevens Cornell University USA; Pankaj Jaiswal Cornell University USA; Ayton Meintjes, Africa do Sul; Mark Wilkinson University of British Columbia Canada; Benjamin Good University Drive Canada; James Wagner University Drive Canada; Jane Morris, Africa do Sul; David Marshall, Inglaterra; Anthony Collins Cip Peru; Shoshi Kikuchi NIAS Japão; Thomas Metz IRRI Filipinas; Graham McLaren IRRI Filipinas; Theo van Hintum CGN Holanda. |
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Título: |
The generation challenge programme platform: semantic standards and workbench for crop science. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
International Journal of Plant Genomics, v.2008, 6 p., 2008. |
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Idioma: |
Inglês |
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Notas: |
doi:10.1155/2008/369601. |
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Palavras-Chave: |
Bioinformática; Programa Geração Challenge. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178425/1/SP-19709-ID-31140.pdf
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Marc: |
LEADER 02064naa a2200817 a 4500
001 1190852
005 2022-10-06
008 2008 bl uuuu u00u1 u #d
100 1 $aBRUSKIEWICH, R.
245 $aThe generation challenge programme platform$bsemantic standards and workbench for crop science.$h[electronic resource]
260 $c2008
500 $adoi:10.1155/2008/369601.
653 $aBioinformática
653 $aPrograma Geração Challenge
700 1 $aSENGER, M.
700 1 $aDAVENPORT, G.
700 1 $aRUIZ, M.
700 1 $aROUARD, M.
700 1 $aHAZEKAMP, T.
700 1 $aTAKEYA, M.
700 1 $aDOI, K.
700 1 $aSATOH, K.
700 1 $aCOSTA, M.
700 1 $aSIMON, R.
700 1 $aBALAJI, J.
700 1 $aAKINTUNDE, A.
700 1 $aMAULEON, R.
700 1 $aWANCHANA, S.
700 1 $aSHAH, T.
700 1 $aANACLETO, M.
700 1 $aPORTUGAL, A.
700 1 $aULAT, V. J.
700 1 $aTHONGJUEA, S.
700 1 $aBRAAK, K.
700 1 $aRITTER, S.
700 1 $aDEREEPER, M.
700 1 $aSKOFIC, M.
700 1 $aROJAS, E.
700 1 $aMARTINS, N.
700 1 $aPAPPAS, G.
700 1 $aALAMBAN, R.
700 1 $aALMODIEL, R.
700 1 $aBARBOZA, L. H.
700 1 $aDETRAS, J.
700 1 $aMANANSALA, K.
700 1 $aMENDOZA, M. J.
700 1 $aMORALES, J.
700 1 $aPERALTA, B.
700 1 $aVALERIO, R.
700 1 $aZHANG, Y.
700 1 $aGREGORIO, S.
700 1 $aHERMOCILLA, J.
700 1 $aECHAVEZ, M.
700 1 $aYAP, J. M.
700 1 $aFARMER, A.
700 1 $aSCHILTZ, G.
700 1 $aLEE, J.
700 1 $aCASSTEVENS, T.
700 1 $aJAISWAL, P.
700 1 $aMEINTJES, A.
700 1 $aWILKINSON, M.
700 1 $aGOOD, B.
700 1 $aWAGNER, J.
700 1 $aMORRIS, J.
700 1 $aMARSHALL, D.
700 1 $aCOLLINS, A.
700 1 $aKIKUCHI, S.
700 1 $aMETZ, T.
700 1 $aMCLAREN, G.
700 1 $aHINTUM, T. van.
773 $tInternational Journal of Plant Genomics$gv.2008, 6 p., 2008.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
17/03/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. |
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Afiliação: |
MARCIO ROBERTO SILVA, CNPGL. |
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Título: |
Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 3, p. 1-6, 2014 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. MenosIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with ... Mostrar Tudo |
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Palavras-Chave: |
Bovine and bubaline tuberculosis; Nested-PCR system. |
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Thesaurus NAL: |
Mycobacterium abscessus. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119848/1/ARAUJO-C.-P..pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99300/1/pone.0091023-1..6-2014-Nested-PCR.pdf
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Marc: |
LEADER 02739naa a2200325 a 4500
001 2010358
005 2024-02-05
008 2014 bl uuuu u00u1 u #d
100 1 $aARAUJO, C. P.
245 $aDetection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1.$h[electronic resource]
260 $c2014
520 $aIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.
650 $aMycobacterium abscessus
653 $aBovine and bubaline tuberculosis
653 $aNested-PCR system
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aRAMOS, C. A. N.
700 1 $aS. FILHO, A. F.
700 1 $aVIDAL, C. E. S.
700 1 $aROXO, E.
700 1 $aNISHIBE, c.
700 1 $aALMEIDA, N. F.
700 1 $aF. JUNIOR, A. A.
700 1 $aSILVA, M. R.
700 1 $aB. NETO, J. D.
700 1 $aCERQUEIRA, V. D.
700 1 $aZUMÁRRAGA, M.
700 1 $aARAUJO, F. R.
773 $tPlos One$gv. 9, n. 3, p. 1-6, 2014
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Embrapa Gado de Leite (CNPGL) |
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