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| Registros recuperados : 87 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
01/08/2016 |
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Data da última atualização: |
01/08/2016 |
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Autoria: |
OBERLENDER, G.; RUIZ LÓPEZ, S.; DE ONDIZ SÁNCHEZ, A. D.; VIEIRA, L. A.; PEREIRA, M. B.; SILVA, L. de F.; ZANGERONIMO, M. G.; MURGAS, L. D. S. |
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Afiliação: |
GUILHERME OBERLENDER, IFSULDEMINAS; SALVARDOR RUIZ LÓPEZ, University of Murcia; AITOR D. DE ORDIZ SÁNCHEZ, University of Zulia; LUIS A. VIEIRA, PPGCV/UECE; MARIANE BARRETO PEREIRA, IFSULDEMINAS; LUANY DE FÁTIMA SILVA, IFSULDEMINAS; MÁRCIO G. ZANGERONIMO, DMV/UFLA; LUIS D. S. MURGAS, DMV/UFLA. |
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Título: |
In vitro fertilization of porcine oocytes is affected by spermatic coincubation time. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 36 (supl. 1), p. 89-93, jun. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim was to study the effects of different gamete coincubation times on porcine in vitro fertilization (IVF), and to verify whether efficiency could be improved by reducing oocyte exposure time to spermatozoa during IVF. In groups of 50, a total of 508 immature cumulus-oocyte complexes (COCs) were matured in NCSU-37 medium. The COCs were cultured for 44 hours and then inseminated with in natura semen (2,000 spermatozoa/ oocyte). The sperm and oocytes were coincubated according to the following treatments (T): T1 = oocytes exposed to spermatozoa for one hour (173 oocytes), T2 = oocytes exposed to spermatozoa for two hours (170 oocytes), and T3 = oocytes exposed to spermatozoa for three hours (165 oocytes). After these coincubation periods, the oocytes were washed in fertilization medium (TALP medium) to remove spermatozoa not bound to the zona pellucida and cultured in another similar medium (containing no sperm). Eighteen to twenty hours after fertilization, the putative zygotes were stained in Hoechst-33342 to evaluate the IVF results. The penetration rate was higher (P<0.05) after two hours of coincubation time than it was for one or three hours. Furthermore, 68.60% of the ova coincubated with the spermatozoa for two hours were monospermic. The oocytes exposed to spermatozoa for one hour (T1) presented a higher (P<0.01) rate of polyspermy than those in T2 and T3. Fertilization performance (%) did not differ (P>0.05) between oocytes exposed to spermatozoa for one (T1) and three hours (T3). However, optimum (P=0.048) results were obtained after two hours of coincubation, when the rate of fertilization performance was 50.16±8.52%. The number of penetrated sperm per oocyte, as well as male pronucleus formation, did not differ (P>0.05) between the treatments evaluated. Under these assay conditions, especially in relation to the sperm concentration used, gamete coincubation for a period of two hours appears to be optimal for monospermy and fertilization performance. Thus, it is the optimal time period for obtaining a large number of pig embryos capable of normal development. MenosThe aim was to study the effects of different gamete coincubation times on porcine in vitro fertilization (IVF), and to verify whether efficiency could be improved by reducing oocyte exposure time to spermatozoa during IVF. In groups of 50, a total of 508 immature cumulus-oocyte complexes (COCs) were matured in NCSU-37 medium. The COCs were cultured for 44 hours and then inseminated with in natura semen (2,000 spermatozoa/ oocyte). The sperm and oocytes were coincubated according to the following treatments (T): T1 = oocytes exposed to spermatozoa for one hour (173 oocytes), T2 = oocytes exposed to spermatozoa for two hours (170 oocytes), and T3 = oocytes exposed to spermatozoa for three hours (165 oocytes). After these coincubation periods, the oocytes were washed in fertilization medium (TALP medium) to remove spermatozoa not bound to the zona pellucida and cultured in another similar medium (containing no sperm). Eighteen to twenty hours after fertilization, the putative zygotes were stained in Hoechst-33342 to evaluate the IVF results. The penetration rate was higher (P<0.05) after two hours of coincubation time than it was for one or three hours. Furthermore, 68.60% of the ova coincubated with the spermatozoa for two hours were monospermic. The oocytes exposed to spermatozoa for one hour (T1) presented a higher (P<0.01) rate of polyspermy than those in T2 and T3. Fertilization performance (%) did not differ (P>0.05) between oocytes exposed to spermatozoa for one (T1) an... Mostrar Tudo |
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Palavras-Chave: |
Coincubation time; Eficiência da fertilização; Fertilização in vitro; Fetilization performance; Ovócito; Pig; Tempo de coincubação. |
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Thesagro: |
Suíno. |
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Thesaurus NAL: |
In vitro fertilization; Oocytes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/145959/1/In-vitro-fertilization.pdf
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Marc: |
LEADER 03080naa a2200325 a 4500
001 2049975
005 2016-08-01
008 2016 bl uuuu u00u1 u #d
100 1 $aOBERLENDER, G.
245 $aIn vitro fertilization of porcine oocytes is affected by spermatic coincubation time.
260 $c2016
520 $aThe aim was to study the effects of different gamete coincubation times on porcine in vitro fertilization (IVF), and to verify whether efficiency could be improved by reducing oocyte exposure time to spermatozoa during IVF. In groups of 50, a total of 508 immature cumulus-oocyte complexes (COCs) were matured in NCSU-37 medium. The COCs were cultured for 44 hours and then inseminated with in natura semen (2,000 spermatozoa/ oocyte). The sperm and oocytes were coincubated according to the following treatments (T): T1 = oocytes exposed to spermatozoa for one hour (173 oocytes), T2 = oocytes exposed to spermatozoa for two hours (170 oocytes), and T3 = oocytes exposed to spermatozoa for three hours (165 oocytes). After these coincubation periods, the oocytes were washed in fertilization medium (TALP medium) to remove spermatozoa not bound to the zona pellucida and cultured in another similar medium (containing no sperm). Eighteen to twenty hours after fertilization, the putative zygotes were stained in Hoechst-33342 to evaluate the IVF results. The penetration rate was higher (P<0.05) after two hours of coincubation time than it was for one or three hours. Furthermore, 68.60% of the ova coincubated with the spermatozoa for two hours were monospermic. The oocytes exposed to spermatozoa for one hour (T1) presented a higher (P<0.01) rate of polyspermy than those in T2 and T3. Fertilization performance (%) did not differ (P>0.05) between oocytes exposed to spermatozoa for one (T1) and three hours (T3). However, optimum (P=0.048) results were obtained after two hours of coincubation, when the rate of fertilization performance was 50.16±8.52%. The number of penetrated sperm per oocyte, as well as male pronucleus formation, did not differ (P>0.05) between the treatments evaluated. Under these assay conditions, especially in relation to the sperm concentration used, gamete coincubation for a period of two hours appears to be optimal for monospermy and fertilization performance. Thus, it is the optimal time period for obtaining a large number of pig embryos capable of normal development.
650 $aIn vitro fertilization
650 $aOocytes
650 $aSuíno
653 $aCoincubation time
653 $aEficiência da fertilização
653 $aFertilização in vitro
653 $aFetilization performance
653 $aOvócito
653 $aPig
653 $aTempo de coincubação
700 1 $aRUIZ LÓPEZ, S.
700 1 $aDE ONDIZ SÁNCHEZ, A. D.
700 1 $aVIEIRA, L. A.
700 1 $aPEREIRA, M. B.
700 1 $aSILVA, L. de F.
700 1 $aZANGERONIMO, M. G.
700 1 $aMURGAS, L. D. S.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 36 (supl. 1), p. 89-93, jun. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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