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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/09/2012 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FEDERICI, M. T.; PICCHI, S. C.; STUCHI, E. S.; FADEL, A. L.; LAIA, M. L.; LEMOS, M. V. F.; LEMOS, E. G. M. |
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Afiliação: |
MARÍA TERESA FEDERICI, INIA; SIMONE CRISTINA PICCHI, UNESP; EDUARDO SANCHES STUCHI, CNPMF; ANDRÉ LUIZ FADEL, ESALQ; MARCELO LUIZ LAIA, UFVJM; MANOEL VICTOR FRANCO LEMOS, UNESP; ELIANA GERTRUDES MACEDO LEMOS, UNESP. |
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Título: |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Electronic Journal of Biotechnology, 2012. |
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ISSN: |
0717-3458 |
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DOI: |
10.2225/vol15-issue3-fulltext-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increas... Mostrar Tudo |
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Palavras-Chave: |
Microarray. |
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Thesagro: |
Xylella Fastidiosa. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $a10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M. T.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCitrus
650 $aXylella Fastidiosa
653 $aMicroarray
700 1 $aPICCHI, S. C.
700 1 $aSTUCHI, E. S.
700 1 $aFADEL, A. L.
700 1 $aLAIA, M. L.
700 1 $aLEMOS, M. V. F.
700 1 $aLEMOS, E. G. M.
773 $tElectronic Journal of Biotechnology, 2012.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
23/07/2012 |
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Data da última atualização: |
23/07/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ANDRADE, S. B. de; LIMA, C. S. M.; GAUTÉRIO, G. R.; RUFATO, A. de R.; RUFATO, L. |
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Afiliação: |
Suélen Braga de ANDRADE, UFPEL; Cláudia Simone Madruga Lima, UFPEL; Giulian Rubira Gautério, UFPEL; ANDREA DE ROSSI RUFATO, CNPUV; LEO RUFATO, UDESC. |
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Título: |
Polinizações interespecíficas para obtenção de seedlings de pereira. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 12.; MOSTRA CIENTÍFICA UFPEL, 3., 2011, Pelotas. [Anais...] [Pelotas: UFPEL, 2011]. |
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Páginas: |
4 p. |
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Idioma: |
Português |
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Palavras-Chave: |
Anais; CNPUV; IC; Iniciação cientifica. |
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Thesagro: |
Fruticultura; Frutificação; Pera; Polinização. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/62237/1/CA-00302.pdf
https://www.ufpel.edu.br/cic/2011/anais/pdf/CA/CA_00302.pdf
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Marc: |
LEADER 00768nam a2200253 a 4500
001 1928842
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008 2011 bl uuuu u00u1 u #d
100 1 $aANDRADE, S. B. de
245 $aPolinizações interespecíficas para obtenção de seedlings de pereira.$h[electronic resource]
260 $aIn: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 12.; MOSTRA CIENTÍFICA UFPEL, 3., 2011, Pelotas. [Anais...] [Pelotas: UFPEL$c2011
300 $a4 p.
650 $aFruticultura
650 $aFrutificação
650 $aPera
650 $aPolinização
653 $aAnais
653 $aCNPUV
653 $aIC
653 $aIniciação cientifica
700 1 $aLIMA, C. S. M.
700 1 $aGAUTÉRIO, G. R.
700 1 $aRUFATO, A. de R.
700 1 $aRUFATO, L.
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