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| Registros recuperados : 14 | |
| 1. |  | ZUMÁRRAGA, M. J.; CATALDI, A.; ARAUJO, F. R.; DIB, C. C.; ROXO, E. Caracterização genética de Mycobacterium bovis em animais de cinco estados brasileiros. O Biológico, São Paulo, SP, v. 74, n. 2, p 95 , jul./dez. 2012. Edição dos resumos da 25ª Reunião Anual do Instituto Biológico, São Paulo, 2012 e 10º Congresso de Iniciação Científica em Ciências Agrárias Biológicas e Ambientais - CICAM, agosto, 2012. Resumo 09.| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | BARBOSA, J. D.; VINHOTE, W. M. S.; PASSOS, M. B.; SOUZA, M. G.; ARAUJO, F. R.; DIB, C. C.; ROXO, E. Ocorrência de tuberculosa e brucelose em búfalos no etado do Pará, Brasil. O Biológico, São Paulo, SP, v. 74, n. 2, p. 96 , jul./dez. 2012. Edição dos resumos da 25ª Reunião Anual do Instituto Biológico, São Paulo, 2012 e 10º Congresso de Iniciação Científica em Ciências Agrárias Biológicas e Ambientais - CICAM, agosto, 2012. Resumo 011.| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | BENESI, F. J.; PINHEIRO, S. R.; MAIORKA, P. C.; SAKAMOTO, S. M.; ROXO, E.; BENITES, N. R.; BIRGEL JÚNIOR, E. H.; GREGORY, L. Relato de caso: tuberculose em caprino (Capra hircus). Arquivos do Instituto Biológico, v. 75, n. 2, p. 217-220, abr./jun. 2008. Comunicação científica.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 6. | | GONCALVES, C. A.; ROXO, E.; CALIL, E. M. B.; KOTAIT, I.; BALDASSI, L.; PORTUGAL, M. A. da S. C.; LAUAR, N. M.; CALIL, R. M.; GIORGI, W. Zoonoses. Campinas: CATI, 1995. 121 p. (CATI. Manual, 31).| Biblioteca(s): Embrapa Acre; Embrapa Agropecuária Oeste; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte; Embrapa Pantanal; Embrapa Semiárido; Embrapa Suínos e Aves; Embrapa Tabuleiros Costeiros. MenosEmbrapa Acre; Embrapa Agropecuária Oeste; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte; Embrapa Pantanal; Embrapa Semiárido; Embrapa Suínos e Aves... Mostrar Todas |
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| 7. | | GONCALVES, C. A.; ROXO, E.; CALIL, E. M. B.; KOTAIT, I.; BALDASSI, L.; PORTUGAL, M. A. da S. C.; LAUAR, N. M.; GIORGI, W. Zoonoses. Campinas: CATI, 1995. 121p. (CATI. Manual, 31)| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 8. | | GONCALVES, C. A.; ROXO, E.; CALIL, E. M. B.; KOTAIT, I.; BALDASSI, L.; PORTUGAL, M. A. da S. C.; LAVAR, N. M.; CALIL, R. M.; GIORGI, W. Zoonoses. Campinas: CATI, 1995. 121p. (CATI. Manual, 31).| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | ARAUJO, C. P.; OSORIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; FILHO, A. F. S.; VIDAL, C. E. S.; ROXO, E.; ROCHA, A.; FONSECA JR. A. A.; SILVA, M. R.; ARAUJO, F. R. Direct detection of Mycobacterium sp in bovine and bubaline tissues by PCR. In: Simpósio Embrapa LabEx EUA de Sanidade Animal, 2., 2012, Brasília. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012 p. 42| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | ABRAMOVAY, R.; FERREIRA, J. N.; COSTA, F. de A.; EHRLICH, M.; EULER, A. M. C.; YOUNG, C. E. F.; KAIMOWITZ, D.; MOUTINHO, P.; NOBRE, I.; ROGEZ, H.; ROXO, E.; SCHOR, T.; VILLANOVA, L. The new bioeconomy in the Amazon: opportunities and challenges for a healthy standing forest and flowing rivers. In: Amazon Assessment Report 2021.| Biblioteca(s): Embrapa Amapá; Embrapa Amazônia Oriental. |
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| 11. | | ARAÚJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; SOUZA FILHO, A. F.; VIDAL, C. E. S.; VARGAS, A. P. C.; ROXO, E; ROCHA, A. S.; SUFFYS, P. N.; FONSECA JÚNIOR, A. A.; SILVA, M. R.; BARBOSA NETO, J. D.; CERQUEIRA, V. D.; ARAUJO, F. R. Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Brazilian Journal of Microbiology 45, 2, 2014, P. 633-640., 2014.| Biblioteca(s): Embrapa Gado de Corte; Embrapa Gado de Leite. |
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| 12. | | ARAÚJO, C. P. de; ARAÚJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; SOUZA FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; DIB, C. C.; ROCHA, A; FONSECA JÚNIOR, A. A.; SILVA, M. R.; ARAUJO, F. R. Diagnóstico de tuberculose por PCR Tempo Real em tecidos de bovinos. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 7., 2011, CAMPO GRANDE, MS. [Anais da]... Campo Grande, MS: Embrapa Gado de Corte, 2011.| Biblioteca(s): Embrapa Gado de Corte. |
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| 13. | | ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. Plos One, v. 9, n. 3, p. 1-6, 2014| Biblioteca(s): Embrapa Gado de Corte; Embrapa Gado de Leite. |
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| 14. | | PATANE, J. S. L.; MARTINS JUNIOR, J.; CASTELÃO, A. B.; NISHIBE, C.; MONTERA, L.; BIGI, F.; ZUMÁRRAGA, M. J.; CATALDI, A. A.; FONSECA JUNIOR, A.; ROXO, E.; OSÓRIO, A. L. A. R.; JORGE, K. S.; THACKER, T. C.; ALMEIDA, N. F.; ARAUJO, F. R.; SETUBAL, J. C. Patterns and Processes of Mycobacterium bovis Evolution Revealed by Phylogenomic Analyses. Genome Biology and Evolution, v. 9, n. 3, p. 521-535, March 2017.| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 14 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
17/03/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. |
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Afiliação: |
MARCIO ROBERTO SILVA, CNPGL. |
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Título: |
Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 3, p. 1-6, 2014 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. MenosIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with ... Mostrar Tudo |
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Palavras-Chave: |
Bovine and bubaline tuberculosis; Nested-PCR system. |
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Thesaurus NAL: |
Mycobacterium abscessus. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119848/1/ARAUJO-C.-P..pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99300/1/pone.0091023-1..6-2014-Nested-PCR.pdf
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Marc: |
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245 $aDetection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1.$h[electronic resource]
260 $c2014
520 $aIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.
650 $aMycobacterium abscessus
653 $aBovine and bubaline tuberculosis
653 $aNested-PCR system
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aRAMOS, C. A. N.
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700 1 $aROXO, E.
700 1 $aNISHIBE, c.
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700 1 $aARAUJO, F. R.
773 $tPlos One$gv. 9, n. 3, p. 1-6, 2014
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Embrapa Gado de Leite (CNPGL) |
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