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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/01/2017 |
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Data da última atualização: |
23/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CALVI, G. P.; AUD, F. F.; FERRAZ, I. D. K.; PRITCHARD, H. W.; KRANNER, I. |
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Afiliação: |
G. P. CALVI, INPA; FABIANA FERRAZ AUD, CNPMF; I. D. K. FERRAZ, INPA; H. W. PRITCHARD, Royal Botanic Gardens; I. KRANNER, Royal Botanic Gardens. |
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Título: |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Biology, n. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017. |
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ISSN: |
1435-8603 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis. MenosThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towar... Mostrar Tudo |
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Thesagro: |
Semente. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02347naa a2200193 a 4500
001 2061470
005 2017-01-23
008 2017 bl uuuu u00u1 u #d
022 $a1435-8603
100 1 $aCALVI, G. P.
245 $aAnalyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination.$h[electronic resource]
260 $c2017
520 $aThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis.
650 $aSemente
700 1 $aAUD, F. F.
700 1 $aFERRAZ, I. D. K.
700 1 $aPRITCHARD, H. W.
700 1 $aKRANNER, I.
773 $tPlant Biology$gn. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
05/03/2024 |
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Data da última atualização: |
05/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TELES, E.; XAVIER, J. F.; ARCENIO, F. S.; AMAYA, R. L.; GONÇALVES, J. V. S.; ROUWS, L. F. M.; ZONTA, E.; COELHO, I. S. |
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Afiliação: |
ERICO TELES, UFRRJ; FRANCIS J. XAVIER, UFRRJ; UFRRJ; UFRRJ; UFRRJ; LUC FELICIANUS MARIE ROUWS, CNPAB; UFRRJ; UFRRJ. |
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Título: |
Characterization and evaluation of potential halotolerant phosphate solubilizing bacteria from Salicornia fruticosa rhizosphere. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 14, article 1324056, 2024. |
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ISSN: |
1664-462X |
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DOI: |
10.3389/fpls.2023.1324056 |
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Idioma: |
Inglês |
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Conteúdo: |
Soil salinization is a significant abiotic factor threatening agricultural production, while the low availability of phosphorus (P) in plants is another worldwide limitation. Approximately 95-99% of the P in soil is unavailable to plants. Phosphate-solubilizing bacteria (PSB) transform insoluble phosphates into soluble forms that plants can utilize. The application of PSB can replace or partially reduce the use of P fertilizers. Therefore, selecting bacteria with high solubilization capacity from extreme environments, such as saline soils, becomes crucial. This study aimed to identify twenty-nine bacterial strains from the rhizosphere of Salicornia fruticosa by sequencing the 16S rDNA gene, evaluate their development in increasing concentrations of NaCl, classify them according to their salinity response, and determine their P solubilization capability. The bacteria were cultivated in nutrient agar medium with NaCl concentrations ranging from 0.5% to 30%. The phosphate solubilization capacity of the bacteria was evaluated in angar and broth National Botanical Research Institute (NBRIP) media supplemented with calcium phosphate (CaHPO4) and aluminum phosphate (AlPO4), and increased with 3% NaCl. All bacterial strains were classified as halotolerant and identified to the genera Bacillus, Enterobacter, Halomonas, Kushneria, Oceanobacillus, Pantoea, Pseudomonas, and Staphylococcus, with only one isolate was not identified. The isolates with the highest ability to solubilize phosphorus from CaHPO4 in the liquid medium were Kushneria sp. (SS102) and Enterobacter sp. (SS186), with 989.53 and 956.37 mg center dot Kg(-1) P content and final pH of 4.1 and 3.9, respectively. For the solubilization of AlPO4, the most effective isolates were Bacillus sp. (SS89) and Oceanobacillus sp. (SS94), which raised soluble P by 61.10 and 45.82 mg center dot Kg(-1) and final pH of 2.9 and 3.6, respectively. These bacteria demonstrated promising results in in vitro P solubilization and can present potential for the development of bioinput. Further analyses, involving different phosphate sources and the composition of produced organic acids, will be conducted to contribute to a comprehensive understanding of their applications in sustainable agriculture MenosSoil salinization is a significant abiotic factor threatening agricultural production, while the low availability of phosphorus (P) in plants is another worldwide limitation. Approximately 95-99% of the P in soil is unavailable to plants. Phosphate-solubilizing bacteria (PSB) transform insoluble phosphates into soluble forms that plants can utilize. The application of PSB can replace or partially reduce the use of P fertilizers. Therefore, selecting bacteria with high solubilization capacity from extreme environments, such as saline soils, becomes crucial. This study aimed to identify twenty-nine bacterial strains from the rhizosphere of Salicornia fruticosa by sequencing the 16S rDNA gene, evaluate their development in increasing concentrations of NaCl, classify them according to their salinity response, and determine their P solubilization capability. The bacteria were cultivated in nutrient agar medium with NaCl concentrations ranging from 0.5% to 30%. The phosphate solubilization capacity of the bacteria was evaluated in angar and broth National Botanical Research Institute (NBRIP) media supplemented with calcium phosphate (CaHPO4) and aluminum phosphate (AlPO4), and increased with 3% NaCl. All bacterial strains were classified as halotolerant and identified to the genera Bacillus, Enterobacter, Halomonas, Kushneria, Oceanobacillus, Pantoea, Pseudomonas, and Staphylococcus, with only one isolate was not identified. The isolates with the highest ability to solubilize phos... Mostrar Tudo |
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Palavras-Chave: |
Rhizobacteria. |
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Thesaurus NAL: |
Phosphorus; Plant growth. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 03068naa a2200265 a 4500
001 2162586
005 2024-03-05
008 2024 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2023.1324056$2DOI
100 1 $aTELES, E.
245 $aCharacterization and evaluation of potential halotolerant phosphate solubilizing bacteria from Salicornia fruticosa rhizosphere.$h[electronic resource]
260 $c2024
520 $aSoil salinization is a significant abiotic factor threatening agricultural production, while the low availability of phosphorus (P) in plants is another worldwide limitation. Approximately 95-99% of the P in soil is unavailable to plants. Phosphate-solubilizing bacteria (PSB) transform insoluble phosphates into soluble forms that plants can utilize. The application of PSB can replace or partially reduce the use of P fertilizers. Therefore, selecting bacteria with high solubilization capacity from extreme environments, such as saline soils, becomes crucial. This study aimed to identify twenty-nine bacterial strains from the rhizosphere of Salicornia fruticosa by sequencing the 16S rDNA gene, evaluate their development in increasing concentrations of NaCl, classify them according to their salinity response, and determine their P solubilization capability. The bacteria were cultivated in nutrient agar medium with NaCl concentrations ranging from 0.5% to 30%. The phosphate solubilization capacity of the bacteria was evaluated in angar and broth National Botanical Research Institute (NBRIP) media supplemented with calcium phosphate (CaHPO4) and aluminum phosphate (AlPO4), and increased with 3% NaCl. All bacterial strains were classified as halotolerant and identified to the genera Bacillus, Enterobacter, Halomonas, Kushneria, Oceanobacillus, Pantoea, Pseudomonas, and Staphylococcus, with only one isolate was not identified. The isolates with the highest ability to solubilize phosphorus from CaHPO4 in the liquid medium were Kushneria sp. (SS102) and Enterobacter sp. (SS186), with 989.53 and 956.37 mg center dot Kg(-1) P content and final pH of 4.1 and 3.9, respectively. For the solubilization of AlPO4, the most effective isolates were Bacillus sp. (SS89) and Oceanobacillus sp. (SS94), which raised soluble P by 61.10 and 45.82 mg center dot Kg(-1) and final pH of 2.9 and 3.6, respectively. These bacteria demonstrated promising results in in vitro P solubilization and can present potential for the development of bioinput. Further analyses, involving different phosphate sources and the composition of produced organic acids, will be conducted to contribute to a comprehensive understanding of their applications in sustainable agriculture
650 $aPhosphorus
650 $aPlant growth
653 $aRhizobacteria
700 1 $aXAVIER, J. F.
700 1 $aARCENIO, F. S.
700 1 $aAMAYA, R. L.
700 1 $aGONÇALVES, J. V. S.
700 1 $aROUWS, L. F. M.
700 1 $aZONTA, E.
700 1 $aCOELHO, I. S.
773 $tFrontiers in Plant Science$gv. 14, article 1324056, 2024.
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