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| Registros recuperados : 128 | |
| 9. |  | ANDRADE, A. C.; ROUWS, L. F. M.; DE WAARD, M. A. Functional characterization of AzoA, a novel molecular determinant of sensitivity to azoles in Aspergillus nidulans. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | ROUWS, L. F. M.; MENESES, C. H. S. G.; VIDAL, M. S.; BALDANI, J. I. Evidence for an important role of the Gluconacetobacter diazotrophics Flp pilus during rice root surface colonization. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SVI.08| Biblioteca(s): Embrapa Agrobiologia. |
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| 16. | | ROUWS, L. F. M.; MENESES, C. H. G.; GUEDES. H. V.; VIDAL, M. S.; SCHWAB, S. Use of reporter genes to monitor interactions between the diazotrophic bacterium Gluconacetobacter diazotrophicus and its host plants sugarcane and rice. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR SBBq, 39.; LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010.| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | MENEZES JÚNIOR, I. de A.; OSEI, O.; MATOS, G. F. de; ROUWS, L. F. M. (Brady)rizóbios associadas à cana-de-açúcar: abundância, diversidade e o efeito do genótipo da planta In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., FERTBIO 2016. Goiânia. Rumo aos novos desafios: anais. Goiânia: UFG, 2016.| Biblioteca(s): Embrapa Agrobiologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
07/03/2023 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SCHWAB, S.; HIRATA, E. S.; AMARAL, J. C. A.; SILVA, C. G. N.; FERREIRA, J. P.; SILVA, L. V. da; ROUWS, J. R. C.; ROUWS, L. F. M.; BALDANI, J. I.; REIS, V. M. |
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Afiliação: |
STEFAN SCHWAB, CNPAB; BASF S.A; J. C. A. AMARAL, UFRRJ; C. G. N. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; J. P. FERREIRA, SOLUBIO; L. V. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; JANAINA RIBEIRO COSTA ROUWS, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB; JOSE IVO BALDANI, CNPAB; VERONICA MASSENA REIS, CNPAB. |
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Título: |
Quantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods, |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant and Soil , Published: 22 February 2023. |
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ISSN: |
1573-5036 |
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Idioma: |
Inglês |
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Conteúdo: |
Nitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion of the mini-setts in bacterial suspension proved to be more efficient than the inoculation methods of applying the suspension onto the post-sprouting substrate or to the lowest lea MenosNitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion o... Mostrar Tudo |
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Palavras-Chave: |
Nitrospirillum amazonense; Promoting plant growth. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02478naa a2200265 a 4500
001 2152151
005 2023-03-07
008 2023 bl uuuu u00u1 u #d
022 $a1573-5036
100 1 $aSCHWAB, S.
245 $aQuantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods,$h[electronic resource]
260 $c2023
520 $aNitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion of the mini-setts in bacterial suspension proved to be more efficient than the inoculation methods of applying the suspension onto the post-sprouting substrate or to the lowest lea
653 $aNitrospirillum amazonense
653 $aPromoting plant growth
700 1 $aHIRATA, E. S.
700 1 $aAMARAL, J. C. A.
700 1 $aSILVA, C. G. N.
700 1 $aFERREIRA, J. P.
700 1 $aSILVA, L. V. da
700 1 $aROUWS, J. R. C.
700 1 $aROUWS, L. F. M.
700 1 $aBALDANI, J. I.
700 1 $aREIS, V. M.
773 $tPlant and Soil , Published: 22 February 2023.
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