| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
08/06/2018 |
|
Data da última atualização: |
29/04/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BOSCO, D. D.; SINSKI, I.; RITSCHEL, P. S.; CAMARGO, U. A.; FAJARDO, T. V. M.; HARAKAVA, R.; QUECINI, V. |
|
Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; Umberto A. Camargo; THOR VINICIUS MARTINS FAJARDO, CNPUV; Ricardo Harakava; VERA MARIA QUECINI, CNPUV. |
|
Título: |
Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Transgenic Research, p. 1-18, 2018. [Online] |
|
DOI: |
https://doi.org/10.1007/s11248-018-0082-1 |
|
Idioma: |
Português |
|
Conteúdo: |
Increased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis MenosIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and... Mostrar Tudo |
|
Palavras-Chave: |
Fungus; Pathogenesis related protein 5. |
|
Thesaurus Nal: |
Chitinase; Epigenetics; Grapevine leafroll-associated virus 3; Vitis. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178409/1/s11248-018-0082-1-Artigo-v-publicada-Metilacao-DNA-Transgenic-Research-06-06-18.pdf
|
|
Marc: |
LEADER 02824naa a2200277 a 4500
001 2092380
005 2019-04-29
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11248-018-0082-1$2DOI
100 1 $aBOSCO, D. D.
245 $aExpression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.$h[electronic resource]
260 $c2018
520 $aIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis
650 $aChitinase
650 $aEpigenetics
650 $aGrapevine leafroll-associated virus 3
650 $aVitis
653 $aFungus
653 $aPathogenesis related protein 5
700 1 $aSINSKI, I.
700 1 $aRITSCHEL, P. S.
700 1 $aCAMARGO, U. A.
700 1 $aFAJARDO, T. V. M.
700 1 $aHARAKAVA, R.
700 1 $aQUECINI, V.
773 $tTransgenic Research, p. 1-18, 2018. [Online]
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Florestas. |
|
Data corrente: |
02/09/2015 |
|
Data da última atualização: |
16/02/2016 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
OLIVEIRA, K. A. de; CARDOSO, D. J.; ROSOT, N. C. |
|
Afiliação: |
Karen Aline de Oliveira, Acadêmica do curso de Engenharia Florestal, UFPR; DENISE JETON CARDOSO, CNPF; Nelson Carlos Rosot, UFPR. |
|
Título: |
Simulações de regimes de desbaste para produção de madeira em plantações de Araucaria angustifolia. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 14., 2015, Colombo. Anais... Colombo: Embrapa Florestas, 2015. |
|
Páginas: |
p. 92-93. |
|
Série: |
(Embrapa Florestas. Documentos, 278). |
|
Idioma: |
Português |
|
Notas: |
Resumo. |
|
Thesagro: |
Crescimento; Pinheiro do Paraná; Volume. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/128984/1/Doc.-278-Cardoso-2.pdf
|
|
Marc: |
LEADER 00691nam a2200193 a 4500
001 2023192
005 2016-02-16
008 2015 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, K. A. de
245 $aSimulações de regimes de desbaste para produção de madeira em plantações de Araucaria angustifolia.$h[electronic resource]
260 $aIn: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 14., 2015, Colombo. Anais... Colombo: Embrapa Florestas$c2015
300 $ap. 92-93.
490 $a(Embrapa Florestas. Documentos, 278).
500 $aResumo.
650 $aCrescimento
650 $aPinheiro do Paraná
650 $aVolume
700 1 $aCARDOSO, D. J.
700 1 $aROSOT, N. C.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Florestas (CNPF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
