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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/12/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, R. L. da; SOUZA, E. H. de; VIEIRA, L. de J.; PELACANI, C. R.; SOUZA, F. V. D. |
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Afiliação: |
RONILZE LEITE DA SILVA, EFS; EVERTON HILO DE SOUZA, CAPES; LÍVIA DE JESUS VIEIRA, CAPES; CLAUDINÉIA REGINA PELACANI, UEFS; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Cryopreservation of pollen of wild pineapple accessions. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Scientia Horticulturae, v.219, p.326-334, May 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Cryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in vitro germination percentages and production of viable seeds. MenosCryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in... Mostrar Tudo |
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Thesagro: |
Abacaxi. |
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Categoria do assunto: |
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Marc: |
LEADER 02070naa a2200181 a 4500
001 2081351
005 2017-12-04
008 2017 bl uuuu u00u1 u #d
100 1 $aSILVA, R. L. da
245 $aCryopreservation of pollen of wild pineapple accessions.$h[electronic resource]
260 $c2017
520 $aCryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in vitro germination percentages and production of viable seeds.
650 $aAbacaxi
700 1 $aSOUZA, E. H. de
700 1 $aVIEIRA, L. de J.
700 1 $aPELACANI, C. R.
700 1 $aSOUZA, F. V. D.
773 $tScientia Horticulturae$gv.219, p.326-334, May 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
13/08/2009 |
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Data da última atualização: |
13/08/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
THOMAZ, D. T.; SANTANA, D. L. de Q.; ROSADO-NETO, G. H.; MEDEIROS, A. C. de S.; ABREU, D. C. A. de; WINK, C.; ROVEDO, S. F. |
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Afiliação: |
Daniel Tonial Thomaz, PUC-PR; Dalva Luiz de Queiroz Santana, Embrapa Florestas; Germano Henrique Rosato-Neto, UFPR; Antonio Carlos de Souza Medeiros, Embrapa Florestas; Daniela Cleide Azavedo de Abreu, UNESP; Charlote Wink, Universidade Federal de Santa. |
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Título: |
Herbivoria de insetos (Coleoptera: Curculionidae) em frutos, sementes e plântulas de guaçatonga (Casearia lasiophylla Eichl), na Região Metropolitana de Curitiba, Paraná, Brasil. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 21., 2006, Recife. Entomologia: da academia à transferência de tecnologia: resumos. Recife: Sociedade Entomológica do Brasil, 2006. |
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Descrição Física: |
CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Mata Atlântica; Praga florestal. |
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Thesagro: |
Guacatonga; Praga; Semente. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00925naa a2200253 a 4500
001 1316206
005 2009-08-13
008 2006 bl --- 0-- u #d
100 1 $aTHOMAZ, D. T.
245 $aHerbivoria de insetos (Coleoptera$bCurculionidae) em frutos, sementes e plântulas de guaçatonga (Casearia lasiophylla Eichl), na Região Metropolitana de Curitiba, Paraná, Brasil.
260 $c2006
300 $cCD-ROM.
650 $aGuacatonga
650 $aPraga
650 $aSemente
653 $aMata Atlântica
653 $aPraga florestal
700 1 $aSANTANA, D. L. de Q.
700 1 $aROSADO-NETO, G. H.
700 1 $aMEDEIROS, A. C. de S.
700 1 $aABREU, D. C. A. de
700 1 $aWINK, C.
700 1 $aROVEDO, S. F.
773 $tIn: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 21., 2006, Recife. Entomologia: da academia à transferência de tecnologia: resumos. Recife: Sociedade Entomológica do Brasil, 2006.
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