Data/hora: 08/06/2024 14:46:18

Biblioteca(s): | Embrapa Café.
Tipo da produção científica: | Resumo em Anais de Congresso
Data corrente: | 18/11/2020
Data da última atualização: | 18/11/2020
Autoria: | SANTOS, F. C.; ROSA, S. D. V. F. da; GROTEWOLD, E.; PADILHA, L.
Afiliação: | The Ohio State University; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; The Ohio State University; LILIAN PADILHA, CNPCa.
Título: | Efficiency of coffee seeds rna extraction protoco using rna integrity number analysis.
Ano de publicação: | 2014
Fonte/Imprenta: | In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 273-274.
Idioma: | Português
Conteúdo: | Coffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation.
Thesagro: | Café; Extração; RNA; Semente.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/217961/1/PB240.pdf
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (1472-1) - UPC - RA - DD -  -  -  - DIS