| |
|
|
| Registros recuperados : 12 | |
| 2. |  | LOPES, S. M.; OLIVEIRA, E. H. de; ARAÚJO, M. C. de. Novos registyros de Phoetalia pallida (Brunner, 1865) para o Brasil e considerações sobre a variação cromática da espécie (Blattaria, Blaberidae, Blaberinae). Acta Amazonica, Manaus, v. 34, n. 4, p. 665-667, out./dez. 2004.| Biblioteca(s): Embrapa Florestas. |
|   |
| 7. | | ARAÚJO, M. C. de; BISCEGLI, C. I.; JORGE, L. A. de C.; FERRAZ, A. C. de O.; HONÓRIO, S. L. Diagnóstico dos efeitos de tratamento térmico na castanha de caju através de tomografia de ressonância magnética. SEMANA INTERNACIONAL DA FRUTICULTURA, FLORICULTURA E AGROINDÚSTRIA-FRUTAL=INTERNATIONAL WEEK OF FRUIT CROP, FLORICULTURE AND AGROINDUSTRY, 9.; SIMPÓSIO DE INOVAÇÕES TECNOLÓGICAS E GERENCIAIS=SYMPOSIUM OF TECHNOLOGICAL AND MANAGERIAL INNOVATIONS, 2., 2002, Fortaleza. FRUTAL 2002. Agribusiness: desafios deste século=Agribusiness: challenges from this century.:. Fortaleza: Embrapa Agroindústria Tropical: SEBRAE, 2002. Não paginado. 1 CD-ROM.| Biblioteca(s): Embrapa Instrumentação. |
| |
| 8. | | GUERRA, A. F.; ROCHA, O. C.; RODRIGUES, G. C.; SANZONOWICZ, C.; SAMPAIO, J. B. R.; SILVA, H. C.; ARAÚJO, M. C. de. Irrigação do cafeeiro no cerrado: estratégia de manejo de água para uniformização de florada. Planaltina, DF: Embrapa Cerrados, 2005. 4 p. (Embrapa Cerrados. Comunicado Técnico, 122).| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Cerrados; Embrapa Unidades Centrais. |
|  |
| 10. | | GUERRA, A. F.; ROCHA, O. C.; RODRIGUES, G. C.; SANZONOWICZ, C.; SAMPAIO, J. B. R.; SILVA, H. C.; ARAÚJO, M. C. de. Manejo da irrigação do cafeeiro, com uso do estresse hídrico controlado, para uniformização de florada. In: SIMPÓSIO BRASILEIRO DE PESQUISA EM CAFEICULTURA IRRIGADA, 8., 2006, Araguari. Anais... Araguari: [Associação dos Cafeicultores de Araguari], 2006. p. 65-68.| Biblioteca(s): Embrapa Cerrados. |
| |
| 12. | | ROCHA, O. C.; GUERRA, A. F.; SILVA, F. A. M.; MACHADO JÚNIOR, J. R. R.; ARAÚJO, M. C. de; SILVA, H. C. Programa para monitoramento de irrigação do caffeiro no cerrado. In: SIMPÓSIO BRASILEIRO DE PESQUISA EM CAFEICULTURA IRRIGADA, 8., 2006, Araguari. Anais... Araguari: [Associação dos Cafeicultores de Araguari], 2006. p. 61-69.| Biblioteca(s): Embrapa Cerrados. |
| |
| Registros recuperados : 12 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
02/03/2016 |
|
Data da última atualização: |
09/05/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
SANTOS, F. C.; CLEMENTE, A. da C. S.; CAIXETA, F.; ROSA, S. D. V. F. da. |
|
Afiliação: |
UDI Pesquisa & Desenvolvimento; UFLA; General Mills Brasil Alimentos Ltda.; STTELA DELLYZETE VEIGA F DA ROSA, SAPC. |
|
Título: |
Endo-ß-mannanase and ß-tubulin gene expression during the final phases of coffee seed maturation. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n. 4, p. 11719-11728, 2015. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee seed maturation. MenosCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee se... Mostrar Tudo |
|
Palavras-Chave: |
QRT-PCR. |
|
Thesagro: |
Coffea Arábica. |
|
Thesaurus NAL: |
Developmental stages. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140475/1/Endo-B-mannanase.pdf
|
|
Marc: |
LEADER 02122naa a2200193 a 4500
001 2039275
005 2019-05-09
008 2015 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. C.
245 $aEndo-ß-mannanase and ß-tubulin gene expression during the final phases of coffee seed maturation.$h[electronic resource]
260 $c2015
520 $aCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee seed maturation.
650 $aDevelopmental stages
650 $aCoffea Arábica
653 $aQRT-PCR
700 1 $aCLEMENTE, A. da C. S.
700 1 $aCAIXETA, F.
700 1 $aROSA, S. D. V. F. da
773 $tGenetics and Molecular Research$gv. 14, n. 4, p. 11719-11728, 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
